MĀTĀPONO

He antigen paninga MP-Ag kei runga i te rohe whakamatautau me te koati anti kiore IgG antibody i runga i te rohe whakahaere, ka mau ki te chromatography membrane i mua. Ko te papa tapanga he mea pani ki te koura colloidal kua tapaina kiore-anti tangata IgM McAb i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te MP-IgM i roto i te tauira ki te koura colloidal tapanga kiore-anti tangata IgM McAb, ka hanga i te matatini aukati. I raro i te mahi a te immunochromatography, ka rere te matatini me te tauira o roto o te membrane nitrocellulose ki te huarahi o te pepa absorbent, i te wa i paahitia e te matatini te rohe whakamatautau, ka honoa ki te MP-Ag paninga antigen, ka hanga "MP-Ag paninga antigen-MP. -IgM-colloidal koura kua tapaina kiore-anti tangata IgM McAb” matatini, i puta he roopu whakamatautau tae ki te rohe whakamatautau. Ko te tauira kino karekau e whakaputa i te roopu whakamatautau na te ngoikore o te uaua mate. Ahakoa kei roto te MP-IgM i te tauira, kaore ranei, ka kitea he karaehe whero ki runga i te rohe whakahaere kounga, e kiia ana he paerewa hinonga o roto.

Tikanga Whakamatau:

Ko te tukanga whakamatautau WIZ-A101 ka kite i nga tohutohu a te kaitirotiro mate mate kawe. Ko te tukanga whakamatautau ataata e whai ake nei:

1. Whakatakotoria nga reagents me nga tauira katoa ki te taha mahana.
2. Tangohia te kaari whakamatautau mai i te putea konumohe, tuu ki runga i te teepu taumata ka tohua.
3. Tāpirihia he 10μL serum, tauira plasma ranei, 20μL tauira toto katoa ranei hei tauira pai o te kaari me te dispette kua whakaratohia, katahi ka tapirihia te 100μL (mo te 2-3 maturuturu) tauira waimeha, timata te wa.
4. Taria mo te iti rawa 10-15 meneti ka panui i te hua, ka muhu te hua i muri i nga meneti 15.