MĀTĀPONO

Ko te membrane o te taputapu whakamatautau ka pania ki te Rōpū A me te antigen adenovirus i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te fluorescence kua tapaina he anti Rōpū A me te adenovirus me te rapeti IgG i mua. I te wa e whakamatautau ana i te tauira pai mo te Rōpū A me te adenovirus, ka hono te Rōpū A me te adenovirus i roto i te tauira ki te fluorescence kua tapaina he anti Rotavirus Group A me te adenovirus, ka hangaia he ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te ahunga o te pepa absorbent. I te paahitanga o te matatini ki te rohe whakamatautau, ka honoa ki te Rōpū Anti-Rotavirus A me te antibody paninga adenovirus, ka hanga he matatini hou. Mena he kino, kaore he Rotavirus Group A me te adenovirus antigen i roto i te tauira, kia kore ai e taea te hanga i nga matatini aukati, kaore he raina whero i te waahi rapu (T). Ahakoa ko te roopu A rotavirus me te adenovirus kei roto i te tauira, ka chromatographed te IgG kiore kua tapaina ki te waahi kounga (C) ka mauhia e te koati anti-kiore IgG antibody. Ka puta he raina whero ki te waahi whakahaere kounga (C). Ko te raina whero te paerewa ka puta i te waahi whakahaere kounga (C) mo te whakawa mena he nui nga tauira me te mea he rite tonu te mahi chromatography. Ka whakamahia ano hei paerewa mana o roto mo nga reagents.

Tikanga Whakamatau:

1. Whakatuwheratia te potae o te ngongo kohinga tauira. Kaua e ringihia te otinga ki roto i te pounamu.
2. Tangohia te rakau tauira, ka kuhu ki roto i te tauira tai (whakamahia ranei he rakau tauira hei kohi mo te 50mg o nga tai), katahi ka whakahokia te rakau tauira, ka wiriwiri, ka ruru pai, ka tuaruatia te mahi kia toru nga wa. Tangohia he waahanga rereke o te tauira taima ia wa. Whai muri i te whakatauira, kuhua te rakau tauira ki roto i te ngongo kohinga taie kei roto te waimeha tauira, ka wiriwirihia te topata. Mena he angiangi ake nga tai o te turoro e mate korere ana, ka taea te whakamahi i te kakau kakau kirihou tuku mo te whakatauira. Ma te whakamahi i te whakatauira paipa tuku, tangohia te tauira taima angiangi mai i te turoro matere, katahi ka tapiri kia 3 nga pata (pee ki te 100uL) ki te ngongo tauira fecal.
3. Kia pai te ruirui i te tauira ka tango i te potae kei runga i te pito whakatete ka waiho ki te taha.
4. I te wa e rongoa ana i te iti o te pāmahana, me whakahoki te kete ki te pāmahana rūma i mua i te whakamahi. Tangohia te kaari whakamatautau mai i te putea konumohe, tuu ki te teepu taumata ka tohua.
5. Tangohia te potae mai i te ngongo tauira ka peia nga pata tuatahi e rua kua waimeha tauira, taapirihia kia 3 nga pata (mo te 100uL) kaua he mirumiru waimeha tauira poutū me te ata ki roto i te puna tauira o te kaari me te dispette kua whakaratohia, timata te wa.
6. Me panui te hua i roto i nga meneti 10-15, a ka muhu i muri i nga meneti 15.