MĀTĀPONO

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te Hp-Ab antibody i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga ki te rewharewha kua tapaina he anti Hp-Ag me te rapeti IgG i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te Hp-Ab i roto i te tauira me te rewharewha kua tapaina he anti Hp-Ag, ka hangaia he ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te ahunga o te pepa absorbent. I te paahitanga o te matatini ki te rohe whakamatautau, ka honoa me te antibody paninga anti Hp-Ag, ka hanga he matatini hou. Mena he kino, kaore he HP antibody i roto i te tauira, kia kore ai e taea te hanga i nga matatini aukati, karekau he raina whero ki te waahi kitea (T). Ko te raina whero te paerewa ka puta i te waahi whakahaere kounga (C) mo te whakawa mena he nui nga tauira me te mea he rite tonu te mahi chromatography. Ka whakamahia ano hei paerewa mana o roto mo nga reagents.

Tikanga Whakamatau:

Tena panuihia te kete whakauru i mua i te whakamatautau.

1. Tangohia te kaari whakamatautau mai i te putea konumohe, tuu ki runga i te teepu taumata ka tohua.

2. Taapirihia kia 2 nga pata o te serum, te tauira plasma ranei (e 3 nga pata ranei o te toto katoa/te maihao tauira toto) ki te whakatau i te pai o te kaari me te dispette kua whakaratohia, katahi ka tapirihia kia 1 topata tauira waimeha, timata te wa.

3. Tatari mo te iti rawa 10-15 meneti ka panui i te hua i roto i te 10-15 meneti. He muhu te hua i muri i te 15 meneti.