Kete Tātari mo Myoglobin (whakamātautau immunochromatographic fluorescence)

Mo te whakamahi tātaritanga in vitro anake

Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu. Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI

Ko te kete tātari mo te myoglobin (fluorescence immunochromatographic assay) he whakamaarama immunochromatographic fluorescence mo te kitenga ine te kukū o te myoglobin (MYO) i roto i te serum tangata, plasma ranei, e whakamahia ana hei awhina i te mate o te mate o te mate myocardial infarction. Ko tenei whakamatautau mo te whakamahi ngaio hauora me te whakamahi ngaio ki te kainga anake.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te anti-MYO antibody i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te fluorescence kua tapaina he anti MYO antibody me te rapeti IgG i mua. I te wa e whakamatautau ana i te tauira, ka hono te antigen MYO i roto i te tauira me te fluorescence kua tapaina he antibody anti MYO, ka hangaia he ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te ahunga o te pepa absorbent. I te paahitanga o te matatini ki te rohe whakamatautau, ka honoa me te anti-MYO paninga antibody, ka hanga he matatini hou. He pai te hono o te taumata MYO ki te tohu rewharewha, a ka kitea te kukū o te MYO i roto i te tauira ma te whakamatautau i te immunoassay fluorescence.