MĀTĀPONO

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti fPSA i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te fluorescence kua tapaina he anti PSA antibody me te rapeti IgG i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen fPSA i roto i te tauira me te fluorescence kua tapaina he anti PSA antibody, ka hanga i te ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te huarahi o te pepa absorbent, ka pahemo te uaua ki te rohe whakamatautau, ka honoa ki te antibody paninga anti fPSA, ka hanga he matatini hou. He pai te hono o te taumata fPSA ki te tohu rewharewha, a ka kitea te kukū o te fPSA i roto i te tauira ma te whakamatautau i te immunoassay fluorescence.

Tikanga Whakamatau:

Tena koa panuihia te pukapuka mahi taputapu me te whakauru mokihi i mua i te whakamatautau.

1. Whakatakotoria nga reagents me nga tauira katoa ki te taha mahana.
2. Whakatuwheratia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga whakahaere o te taputapu, ka uru ki te atanga kitenga.
3. Matawaihia te tohu tohu hei whakau i te mea whakamatautau.
4. Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia te 40μL serum, te tauira plasma ranei ki roto i te tauira diluent, ka uru pai.
7. Tāpirihia he otinga tauira 80μL hei tauira pai o te kaari.
8. Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 15, ka kitea aunoa e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
9. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).