MĀTĀPONO

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te FOB antibody i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te rewharewha kua tapaina ki te anti FOB antibody me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen FOB i roto i te tauira me te fluorescence kua tapaina he antibody anti FOB, ka hanga he ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te ahunga o te pepa absorbent. I te paahitanga o te uaua ki te rohe whakamatautau, ka honoa me te antibody paninga anti FOB, ka hanga he matatini hou. Mena he kino, kaore he hemoglobin tangata i roto i te tauira, he iti ranei te ihirangi, kaore e taea te hanga i nga matatini aukati. Karekau he raina whero i te waahi kitea (T). Ko te raina whero te paerewa ka puta i te waahi whakahaere kounga (C) mo te whakawa mena he nui nga tauira me te mea he rite tonu te mahi chromatography. Ka whakamahia ano hei paerewa mana o roto mo nga reagents.

Tikanga Whakamatau:

Tena panuihia te kete whakauru i mua i te whakamatautau.

1.Tangohia te kaari whakamatautau mai i te putea pepa, tuu ki runga i te teepu taumata ka tohu.

2.Tangohia te potae mai i te ngongo tauira ka peia nga pata tuatahi e rua kua waimeha tauira, taapirihia kia 3 nga pata (mo te 100uL) kaore he mirumiru waimeha tauira poutū me te ata ki roto i te puna tauira o te kaari me te dispette kua whakaratohia. Na ka timata te matawā.

3.Me panui te hua i roto i nga meneti 10-15, a ka muhu i muri i nga meneti 15.