Kete tātari moHormone Luteinizingte whakamatautau immunochromatographic fluorescence)
Mo te whakamahi tātaritanga in vitro anake
Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu. Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI
Ko te Kete Tohu mo te Hormone Luteinizing (fluorescence immunochromatographic assay) he whakamatautau immunochromatographic fluorescence mo te kitenga ine o Luteinizing Hormone (LH) i roto i te serum tangata, plasma ranei, e whakamahia ana i te nuinga o te waa ki te arotake i te mahi endocrine pituitary. etahi atu tikanga. Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.

KAUPAPA

Ko te hormone Luteinizing (LH) he glycoprotein me te taumaha ngota mo te 30,000 Dalton, he mea hanga e te pituitary o mua. Ko te kukū o te LH he hononga tata ki te ovulation o ovaries, a ko te tihi o te LH e tohuhia ana ko te 24 ki te 36 haora o te kohungahunga. Na reira, ka taea te aro turuki i te uara teitei o te LH i te wa o te paheketanga ki te whakatau i te wa tino pai o te haputanga. Ko te mahi endocrine rereke i roto i te repe pituitary ka taea te whakapohehe i te hunanga LH. Ka taea te whakamahi i te kukū o te LH ki te arotake i te mahi endocrine pituitary. Ko te Kete Tatautanga kei runga i te immunochromatography ka taea te whakaputa hua i roto i te 15 meneti.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti LH i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te rewharewha kua tapaina he anti-LH antibody me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen LH i roto i te tauira me te fluorescence kua tapaina he anti-LH antibody, ka hangaia he ranunga aukati. I raro i te mahi a te immunochromatography, ko te rerenga matatini ki te huarahi o te pepa absorbent, ka pahemo te uaua ki te rohe whakamatautau, ka honoa ki te antibody paninga anti LH, ka hanga he matatini hou. He pai te hono o te taumata LH ki te tohu rewharewha, a ka kitea te kukū o te LH i roto i te tauira ma te whakamatautau i te immunoassay fluorescence.

NGA REAGENTS ME NGA MAUTANGA I TUKUA

25T nga waahanga kete：

.Kari whakamatautau takitahi he konumohe kua mau ki te whakamaroke 25T
.Tauira waimeha
.Package whakauru

NGĀ KAUPAPA KAUPAPA ENGARI KAORE I TE WHAKAMAHI
ipu kohinga tauira, taima

KOHI TAUIRA ME TE PUTANGA
1.Ko nga tauira whakamatautau ka taea te serum, heparin anticoagulant plasma ranei EDTA anticoagulant plasma.
2.E ai ki nga tikanga paerewa kohikohi tauira. Ka taea te pupuri i te serum, te tauira plasma ranei i roto i te pouaka whakamahana i te 2-8 ℃ mo te 7 ra me te cryopreservation i raro -15°C mo te 6 marama.
3.Katoa nga tauira ka karo i nga huringa whakatio-rewa.

TE WHAKAMAHI WHAKAMAHI

Ko te tikanga whakamatautau o te taputapu tirohia te pukapuka immunoanalyzer. Ko te tukanga whakamatautau reagent e whai ake nei
1.Whakatakotohia nga reagents me nga tauira katoa ki te pāmahana rūma.
2.Whakatuwherahia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga mahi o te taputapu, ka uru ki te atanga kitenga.
3.Matawaia te tohu tohu hei whakau i te mea whakamatautau.
4.Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia he 20μL serum, he tauira plasma ranei hei tauira i te waimeha, ka uru pai.
7. Tāpirihia he otinga tauira 80μL hei tauira pai o te kaari.
8.Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 15, ka kitea e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
9. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).

WHAKAMAHI ME TE WHAKAMAHI

.Ko nga raraunga i runga ake nei ko te waahi tohutoro kua whakaritea mo nga raraunga rapunga o tenei kete, me te whakaaro me whakatu e ia taiwhanga he waahi tohutoro mo te hiranga haumanu e tika ana mo te taupori o tenei rohe.
.Ko te kukū o te LH he teitei ake i te awhe tohutoro, a me whakakorehia nga huringa whaiaroaro, te whakautu ahotea ranei.Ko te mea he rereke, me whakakotahi te tohu tohu haumanu.
.Ko nga hua o tenei tikanga e pa ana ki te awhe tohutoro kua whakaritea e tenei tikanga, a, ko nga hua kaore e tino rite ki etahi atu tikanga.
.Ka taea hoki e etahi atu take te hapa i nga hua kitenga, tae atu ki nga take hangarau, nga hapa whakahaere me etahi atu tauira tauira.

TE WHAKAPUTANGA ME TE TATAU
1.Ko te kete he 18 marama te roa o te oranga mai i te ra i hangaia ai. Whakaorangia nga kete kaore i whakamahia ki te 2-30°C. KAUA E WHAKATAHI. Kaua e whakamahi i tua atu i te ra paunga.
2.Kaua e whakatuwheratia te putea hiri kia rite ra ano koe ki te mahi i tetahi whakamatautau, a ko te whakamatautau kotahi-whakamahi ka whakaarohia kia whakamahia i raro i te taiao e hiahiatia ana (te mahana 2-35 ℃, te haumākū 40-90%) i roto i te 60 mins tere. ka taea.
3.Ka whakamahia te diluent tauira i muri tonu i te whakatuwheratanga.

WHAKATOKANGA ME TE WHAKATAKI
.Me hiri te kete me te tiaki i te makuku.
.Ko nga tauira pai katoa ka whakamanahia e etahi atu tikanga.
.Ko nga tauira katoa ka kiia he poke pea.
KAUA e whakamahi reagent kua pau.
. KAUA e whakawhiti i nga reagents i waenga i nga kete me nga rota rereke No..
.KAUA e whakamahi ano i nga kaari whakamatautau me nga taputapu tuku.
.Ko te mahi pohehe, te taikaha, te iti ranei o te tauira ka puta he rerekee.

LWHAKATAHI
.Ka rite ki nga whakamatautau e whakamahi ana i nga paturopi kiore, tera pea ka pokanoa e nga paturopi anti-kiore tangata (HAMA) i roto i te tauira. Ko nga tauira mai i nga turoro kua whiwhi i nga whakaritenga o nga paturopi monoclonal mo te taatai ​​​​me te rongoa kei roto pea te HAMA. Ko enei tauira ka puta he hua pai, he hua kino ranei.
.Ko tenei hua whakamatautau mo te tohutoro haumanu anake, kaua e waiho hei kaupapa anake mo te taatai ​​haumanu me te maimoatanga, ko te whakahaere haumanu o nga turoro me whai whakaaro matawhānui me ona tohu, hitori hitori, etahi atu whakamatautau taiwhanga, whakautu maimoatanga, mate urutomo me etahi atu korero .
.Ka whakamahia noa tenei reagent mo nga whakamatautau serum me te plasma. Kaore pea e puta he hua tika ina whakamahia mo etahi atu tauira penei i te huware me te mimi me etahi atu.

NGA KAUPAPA KAUPAPA

WHAKAMAHI
1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ko te Nature of Heterophilic Antibodies and the Role in Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.

Te matua mo nga tohu e whakamahia ana:

	In Vitro Diagnostic Medical Device
	Kaihanga
	Rokiroki ki te 2-30 ℃
	Te Ra Whakamutunga
	Kaua e Whakamahi Anō
	WHAKATAHI
	Tirohia nga Tohutohu mo te Whakamahi

Xiamen Wiz Biotech CO.,LTD
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