Kete tātaritanga mopūmua C-reactive hypersensitive

(whakamatautau immunochromatographic fluorescence)

Mo te whakamahi tātaritanga in vitro anake

Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu. Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI

Ko te Kete Tohu mo te pūmua C-reactive hypersensitive (fluorescence immunochromatographic assay) he whakamatautau immunochromatographic fluorescence mo te kitenga ine o te pūmua C-reactive (CRP) i roto i te serum tangata / plasma / Toto katoa. He tohu kore-motuhake mo te mumura. Ko nga tauira pai katoa me whakamana e etahi atu tikanga. Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.

KAUPAPA

Ko te pūmua C-reactive he pūmua wāhanga whakapeka i hangaia e te whakaihiihi lymphokine o te ate me nga pūtau epithelial. Kei roto i te serum tangata, te wai cerebrospinal, te pleural me te wai puku, me etahi atu, he waahanga o te tikanga aukati kore-motuhake. 6-8h i muri i te puta o te mate huakita, ka timata te CRP ki te piki ake, 24-48h ka tae ki te tihi, a ka taea e te uara teitei te eke ki nga rau o nga wa noa. I muri i te whakakorenga o te mate, ka tino heke te CRP, ka hoki ki te tikanga i roto i te wiki kotahi. Engari, karekau te CRP e tino piki ake i roto i te keehi o te mate huaketo, e whakarato ana i te kaupapa mo te tautuhi i nga momo mate moata o nga mate, a he taputapu mo te tautuhi i nga mate huaketo, huakita ranei.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti CRP i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te fluorescence kua tapaina he anti CRP antibody me te rapeti IgG i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen CRP i roto i te tauira me te fluorescence kua tapaina he antibody anti CRP, ka hanga i te ranunga aukati. I raro i te mahi a te immunochromatography, ko te rerenga matatini ki te huarahi o te pepa absorbent, ka pahemo te uaua ki te rohe whakamatautau, ka honoa me te antibody paninga anti CRP, ka hanga he matatini hou. He pai te hono o te taumata CRP ki te tohu rewharewha, a ka kitea te kukū o te CRP i roto i te tauira ma te tātari immunoassay fluorescence.