Kete Tātari mo D-Dimer (whakamātautau immunochromatographic fluorescence)
Kete tātari mo D-Dimer(whakamatautau immunochromatographic fluorescence)
Mo te whakamahi tātaritanga in vitro anake
Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu. Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.
WHAKAMAHI WHAKAMAHI
Ko te kete taatai mo te D-Dimer (te whakamaarama immunochromatographic fluorescence) he whakamatautau immunochromatographic fluorescence mo te kitenga inenga o D-Dimer (DD) i roto i te plasma tangata, ka whakamahia mo te taatai o te thrombosis venous, te tohatoha intravascular coagulation, me te aro turuki i te thrombolytic. therapy .Me whakau nga tauira pai katoa e etahi atu tikanga. Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.
KAUPAPA
Ko te DD e whakaatu ana i te mahi fibrinolytic.Ko nga take mo te piki ake o te DD: 1. Ko te hyperfibrinolysis tuarua, penei i te hypercoagulation, te tohatoha intravascular coagulation, te mate renal, te whakakore i te okana, te thrombolytic therapy, me etahi atu. ; 3. Ko te mate o te ngakau, te mate o te roro, te embolism pulmonary, te thrombosis venous, te pokanga, te puku, te tohatoha intravascular coagulation, te mate me te necrosis kiko, etc
MĀTĀPONO O TE TIKANGA
Ko te kiriuhi o te taputapu whakamatautau ka pania ki te anti DD antibody i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te rewharewha kua tapaina he anti DD antibody me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen DD i roto i te tauira me te fluorescence kua tapaina he anti DD antibody, ka hangaia he ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te huarahi o te pepa absorbent, ka pahemo te uaua ki te rohe whakamatautau, ka honoa ki te antibody paninga anti DD, ka hanga he matatini hou. He pai te hono o te taumata DD ki te tohu rewharewha, a ka kitea te kukū o te DD i roto i te tauira ma te whakamatautau i te immunoassay fluorescence.
NGA REAGENTS ME NGA MAUTANGA I TUKUA
25T nga waahanga kete:
Kari whakamatautau takitahi he konumohe kua mau ki te whakamaroke 25T
Tauira waimeha 25T
Kōkuhu mōkihi 1
NGĀ KAUPAPA KAUPAPA ENGARI KAORE I TE WHAKAMAHI
ipu kohinga tauira, taima
KOHI TAUIRA ME TE PUTANGA
.Ko nga tauira i whakamatauria ka taea te heparin anticoagulant plasma ranei EDTA anticoagulant plasma.
.E ai ki nga tikanga paerewa kohikohi tauira. Ka taea te pupuri i te serum, te tauira plasma ranei i roto i te pouaka whakamahana i te 2-8 ℃ mo te 7 ra me te cryopreservation i raro -15°C mo te 6 marama.
.Katoa nga tauira ka karo i nga huringa whakatio-rewa.
TE WHAKAMAHI WHAKAMAHI
Tena koa panuihia te pukapuka mahi taputapu me te whakauru mokihi i mua i te whakamatautau.
1.Whakatakotohia nga reagents me nga tauira katoa ki te pāmahana rūma.
2.Whakatuwherahia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga mahi o te taputapu, ka uru ki te atanga kitenga.
3.Matawaia te tohu tohu hei whakau i te mea whakamatautau.
4.Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia he 40μL serum, he tauira plasma ranei hei tauira i te waimeha, ka uru pai.
7. Tāpirihia he otinga tauira 80μL hei tauira pai o te kaari.
8.Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 15, ka kitea e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
9. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).
NGA UARA E TIKAIA
DD <0.5mg/L
E taunaki ana kia whakaritea e ia taiwhanga tana ake awhe noa hei tohu mo te taupori turoro.
WHAKAMAHI ME TE WHAKAMAHI
.Ko nga raraunga i runga ake nei ko te hua o te whakamatautau DD reagent, a ko te whakaaro me whakatu e ia taiwhanga te whānuitanga o nga uara rapunga DD e tika ana mo te taupori o tenei rohe. Ko nga hua i runga ake nei hei tohutoro anake.
.Ko nga hua o tenei tikanga e pa ana ki nga awhe tohutoro kua whakaritea i roto i tenei tikanga, a kaore he whakataurite tika ki etahi atu tikanga.
.Ka taea hoki e etahi atu take te hapa i nga hua kitenga, tae atu ki nga take hangarau, nga hapa whakahaere me etahi atu tauira tauira.
TE WHAKAPUTANGA ME TE TATAU
1.Ko te kete he 18 marama te roa o te oranga mai i te ra i hangaia ai. Whakaorangia nga kete kaore i whakamahia ki te 2-30°C. KAUA E WHAKATAHI. Kaua e whakamahi i tua atu i te ra paunga.
2.Kaua e whakatuwheratia te putea hiri kia rite ra ano koe ki te mahi i tetahi whakamatautau, a ko te whakamatautau kotahi-whakamahi ka whakaarohia kia whakamahia i raro i te taiao e hiahiatia ana (te mahana 2-35 ℃, te haumākū 40-90%) i roto i te 60 mins tere. ka taea.
3.Ka whakamahia te diluent tauira i muri tonu i te whakatuwheratanga.
WHAKATOKANGA ME TE WHAKATAKI
1.Me hiri te kete me te tiaki i te makuku.
2.Ko nga tauira pai katoa ka whakamanahia e etahi atu tikanga.
3. Ko nga tauira katoa ka waiho hei poke.
4. KAUA e whakamahi reagent kua pau.
5. KAUA e whakawhiti reagents i waenga i nga kete me te rota rereke No.
6. KAUA e whakamahi ano i nga kaari whakamatautau me nga taputapu tuku.
7.Ko te mahi he, he nui, he iti noa ranei te tauira ka arahi ki nga rereketanga hua.
LWHAKATAHI
.Ka rite ki nga whakamatautau e whakamahi ana i nga paturopi kiore, tera pea ka pokanoa e nga paturopi anti-kiore tangata (HAMA) i roto i te tauira. Ko nga tauira mai i nga turoro kua whiwhi i nga whakaritenga o nga paturopi monoclonal mo te taatai me te rongoa kei roto pea te HAMA. Ko enei tauira ka puta he hua pai, he hua kino ranei.
.Ko tenei hua whakamatautau mo te tohutoro haumanu anake, kaua e waiho hei kaupapa anake mo te taatai haumanu me te maimoatanga, ko te whakahaere haumanu o nga turoro me whai whakaaro matawhānui me ona tohu, hitori hitori, etahi atu whakamatautau taiwhanga, whakautu maimoatanga, mate urutomo me etahi atu korero .
.Ka whakamahia noa tenei reagent mo nga whakamatautau serum me te plasma. Kaore pea e puta he hua tika ina whakamahia mo etahi atu tauira penei i te huware me te mimi me etahi atu.
NGA KAUPAPA KAUPAPA
Rarangirangi | 0.2mg/L ki te10mg/L | inenga whanaunga:-15% ki te +15%. |
Ko te whakarea hononga raina:(r)≥0.9900 | ||
Tika | Ko te tere whakaora i roto i te 85% - 115%. | |
Te tukurua | CV≤15% | |
Te motuhake(Kaore tetahi o nga matū o te pokanoa i whakamatauria i pokanoa ki te whakamatautau) | Whakararuraru | Te kukū pokanoa |
FDP | 120mg/L | |
VC | 2000mg/L | |
Waikawa barbituric | 100mg/L |
RWHAKAMAHI
1.Hansen JH,et al.HAMA Interference with Murine Monoclonal Antibody-Based Immunoassays[J].J of Clin Immunoassay,1993,16:294-299.
2.Levinson SS.Ko te Nature of Heterophilic Antibodies and the Role in Immunoassay Interference[J].J of Clin Immunoassay,1992,15:108-114.
Te matua mo nga tohu e whakamahia ana:
In Vitro Diagnostic Medical Device | |
Kaihanga | |
Rokiroki ki te 2-30 ℃ | |
Te Ra Whakamutunga | |
Kaua e Whakamahi Anō | |
WHAKATAHI | |
Tirohia nga Tohutohu mo te Whakamahi |
Xiamen Wiz Biotech CO.,LTD
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