Kete Tohu（Colloidal Gold）mo Calprotectin
Mo te whakamahi tātaritanga in vitro anake
Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu. Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI
Ko te Kete Tatautanga mo Calprotectin(cal) he karaehe koura immunochromatographic assay mo te whakatau semiquantitative o te cal mai i nga tai tangata, he uara taapiri nui mo te mate whekau. Ko tenei whakamatautau he reagent tirotiro. Ko nga tauira pai katoa me whakamana e etahi atu tikanga. Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake. I tenei wa, ka whakamahia tenei whakamatautau mo te IVD, kaore e hiahiatia he taputapu taapiri.

KAUPAPA
He heterodimer a Cal, he mea tito mai i te MRP 8 me te MRP 14. Kei roto i te neutrophils cytoplasm me te whakaatu ki nga kiriuhi pūtau mononuclear. Ko te Cal he pūmua o te wahanga whakapeka, he pai te wa e noho pumau ana mo te kotahi wiki i roto i nga tai tangata, ka whakatauhia he tohu mo te mate whekau. Ko te kete he whakamatautau whakakitenga ahua ngawari e kitea ana te cal i roto i nga tai tangata, he teitei te maaramatanga me te tino kaha. Ko te whakamatautau i runga i te maataapono tauhohenga o te hanawiti paturopi rua me nga hangarau tātaritanga immunochromatographic koura, ka puta he hua i roto i te 15 meneti.

MĀTĀPONO O TE TIKANGA
He paninga anti-cal McAb kei runga i te rohe whakamatautau me te koati anti-rabbit IgG antibody i runga i te rohe whakahaere, he mea mau ki te chromatography membrane i mua. Ko te papa tapanga he mea pani ki te koura colloidal kua tapaina ki te anti cal McAb me te colloidal koura kua tapaina ki te rapeti IgG antibody i mua. I te wa e whakamatautau ana i te tauira pai, ka uru mai te cal i roto i te tauira ki te koura colloidal kua tapaina he anti cal McAb, ka hanga i te matatini aukati, na te mea ka taea te heke i te taha o te tarai whakamatautau, ka mau te cal conjugate complex e te paninga anti cal McAb ki te kiriuhi me te ahua. Ko te “anti cal coating McAb-cal-colloidal gold labeled anti cal McAb” matatini, i puta he roopu whakamatautau tae ki te rohe whakamatautau. Ko te kaha o te tae he pai te hono ki te ihirangi cal. Ko te tauira toraro karekau e whakaputa i te roopu whakamatautau na te kore o te cal complex conjugate koura colloidal. Ahakoa he tauira kei roto i te tauira kaore ranei, ka kitea he karaehe whero ki te rohe tohutoro me te rohe whakahaere kounga, e kiia ana he paerewa hinonga o-roto kounga.

NGA REAGENTS ME NGA MAUTANGA I TUKUA
25T nga waahanga kete：
.Kari whakamatautau takitahi ko te konumohe i mau ki te whakamaroke
.Tauira waimeha: ko nga kai he 20mM pH7.4PBS
.Dispette
.Package whakauru

NGĀ KAUPAPA KAUPAPA ENGARI KAORE I TE WHAKAMAHI

ipu kohinga tauira, taima

KOHI TAUIRA ME TE PUTANGA
Whakamahia he ipu pokekore hei kohi tauira taima hou, ka whakamatau tonu. Ki te kore e taea te whakamatautau tonu, me rongoa ki te 2-8°C mo te 12 haora, kei raro ranei -15°C mo te 4 marama.

TE WHAKAMAHI WHAKAMAHI
1.Tangohia te rakau tauira, ka kuhu ki roto i te tauira tai, katahi ka whakahokia te rakau tauira, ka wiriwiri kia pai te wiri, ka tuaruatia te mahi kia toru nga wa. Ma te whakamahi ranei i te tauira ka kohia mo te 50mg tauira taima, ka maka ki roto i te ngongo tauira taima kei roto te waimeha tauira, ka wiriwiri.
2.Whakamahia te paipa paipa tango i te tauira taima angiangi mai i te turoro matere, katahi ka tapiri kia 3 nga pata (mo te 100uL) ki te ngongo tauira taie ka wiri pai, waiho ki te taha.
3.Tangohia te kaari whakamatautau mai i te putea pepa, tuu ki runga i te teepu taumata ka tohu.
4.Tangohia te potae mai i te ngongo tauira ka peia nga pata tuatahi e rua kua diluted tauira, tāpirihia kia 3 pata (mo te 100uL) kaore he mirumiru waimeha tauira poutū me te ata ki te tauira pai o te kaari me te dispette kua whakaratohia, timata te wa.
5.Me panui te hua i roto i nga meneti 10-15, a ka muhu i muri i nga meneti 15.

WHAKAMAHI ME TE WHAKAMAHI

TE WHAKAPUTANGA ME TE TATAU
He 24 marama te roa o te kete mai i te ra i hangaia ai. Whakaorangia nga kete kaore i whakamahia ki te 2-30°C. Kaua e whakatuwheratia te putea kua hiri kia rite ra ano koe ki te mahi whakamatautau.
WHAKATOKANGA ME TE WHAKATAKI
1.Me hiri te kete me te tiaki i te makuku¹.
2.Kaua e whakamahi tauira kua roa rawa atu, kua tukuruatia ranei te mīti me te rewa hei whakamatautau
3. He nui rawa nga tauira o te fecal, ka taea ranei e te matotoru te hanga i nga tauira kua waimeha ki te kaari whakamatautau kino, koa centrifuge te tauira waimeha ka tango i te supernatant hei whakamatautau.
4.Misoperation, tauira taikaha iti ranei e taea arahi ki ine hua.

WHAKATAHI
1.Ko tenei hua whakamatautau mo te tohutoro haumanu anake, kaua e waiho hei kaupapa anake mo te taatai ​​haumanu me te maimoatanga, ko te whakahaere haumanu o nga turoro me whai whakaaro matawhānui me ona tohu, hitori hitori, etahi atu whakamatautau taiwhanga, whakautu maimoatanga, epidemiology me etahi atu nga korero².
2.Ka whakamahia noa tenei reagent mo nga whakamatautau fecal. Kaore pea e puta he hua tika ina whakamahia mo etahi atu tauira penei i te huware me te mimi me etahi atu.