Kete Tohu mo te Procalcitonin

(whakamatautau immunochromatographic fluorescence)

Mo te whakamahi tātaritanga in vitro anake

Tena koa panuihia tenei kete whakauru i mua i te whakamahi me te whai i nga tohutohu. Kare e taea te whakapumau i te pono o nga hua whakamatautau mena he rereke nga tohutohu kei roto i tenei kete whakauru.

WHAKAMAHI WHAKAMAHI

Ko te Kete Tohu mo te Procalcitonin (te whakamaarama immunochromatographic fluorescence) he whakamaarama immunochromatographic fluorescence mo te kitenga ine o te Procalcitonin (PCT) i roto i te serum tangata, plasma ranei, ka whakamahia mo te taatai ​​awhina mo te mate huakita me te sepsis. Ko nga tauira pai katoa me whakamana e etahi atu tikanga. Ko tenei whakamatautau he tikanga mo te whakamahi ngaio hauora anake.

KAUPAPA

Ko te Procalcitonin he 116 nga waikawa amino me tona taumaha ngota he 12.7KD. Ko te PCT e whakaatuhia ana e nga pūtau neuroendocrine, ka pakaruhia e nga enzymes ki roto i te calcitonin (kaore i maoa), te peptide whakamutu carboxy, me te peptide whakamutu amino. He iti noa te PCT o te hunga hauora i roto i o ratou toto, ka tino piki ake i muri i te mate huakita. I te wa e pa ana te sepsis i roto i te tinana, ka taea e te nuinga o nga kopa te whakaatu i te PCT, na reira ka taea te whakamahi i te PCT hei tohu tohu mo te sepsis. Mo etahi o nga turoro he mate mumura, ka taea te whakamahi PCT hei tohu mo te kowhiringa paturopi me te whakatau whaihua.

MĀTĀPONO O TE TIKANGA

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti PCT i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te rewharewha kua tapaina he anti-PCT antibody me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen PCT i roto i te tauira me te fluorescence kua tapaina he anti-PCT antibody, ka hangaia he ranunga aukati. I raro i te mahi o te immunochromatography, te rere matatini i roto i te ahunga o te pepa absorbent, ka pahemo matatini te rohe whakamātautau, ngā reira ki anti PCT paninga antibody, hanga matatini hou. He pai te hono o te taumata PCT ki te tohu rewharewha, a ka kitea te kukū o te PCT i roto i te tauira ma te whakamatautau i te immunoassay fluorescence.