MĀTĀPONO

Ko te membrane o te taputapu whakamatautau ka pania ki te Tf antibody i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga ki te rewharewha kua tapaina he anti Tf antibody me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen Tf i roto i te tauira me te fluorescence kua tapaina he anti Tf antibody, ka hanga he ranunga aukati. I raro i te mahi a te immunochromatography, ka rere te matatini ki te ahunga o te pepa absorbent. I te paahitanga o te matatini ki te rohe whakamatautau, ka honoa me te antibody paninga anti Tf, ka hanga he matatini hou.

Mena he kino, kaore he whakawhitinga i roto i te tauira, he iti ranei te ihirangi, kia kore ai e taea te hanga i te matatini aukati. Karekau he raina whero i te waahi kitea (T). Ahakoa kei roto te Tf antibody i roto i te tauira, kaore ranei, ko te toenga o te kiore colloidal-koura kua tapaina ki te kiore anti-tangata IgM monoclonal antibody me te koati anti-kiore IgG antibody kua pania ki te raina rohe whakahaere kounga (C). Na ka whakawhanake nga agglutinates i te tae i roto i te waahanga whakahaere kounga, ka puta te raina whero ki roto (C). Ko te raina whero te paerewa ka puta i te waahi whakahaere kounga (C) mo te whakawa mena he nui nga tauira me te mea he rite tonu te mahi chromatography. Ka whakamahia ano hei paerewa mana o roto mo nga reagents.

Te Tikanga Whakamatau

Tena panuihia te kete whakauru i mua i te whakamatautau.

1.Tangohia te kaari whakamatautau mai i te putea pepa, tuu ki runga i te teepu taumata ka tohu.

2.Tangohia te potae mai i te ngongo tauira ka peia nga pata tuatahi e rua kua waimeha tauira, taapirihia kia 3 nga pata (mo te 100uL) kaore he mirumiru waimeha tauira poutū me te ata ki roto i te puna tauira o te kaari me te dispette kua whakaratohia. Na ka timata te matawā.