MĀTĀPONO

Ko te kiriuhi o te taputapu whakamatautau ka pania ki te antibody anti AFP i runga i te rohe whakamatautau me te koati anti rabbit IgG antibody i runga i te rohe whakahaere. Ka whakakikoruatia te papa tapanga e te rewharewha kua tapaina ki te antibody anti AFP me te IgG rapeti i mua. I te wa e whakamatautau ana i te tauira pai, ka hono te antigen AFP i roto i te tauira me te fluorescence kua tapaina he antibody anti AFP, ka hanga he ranunga aukati. I raro i te mahi o te immunochromatography, te rere matatini i roto i te ahunga o te pepa absorbent, ka pahemo matatini te rohe whakamātautau, ngā reira ki anti AFP paninga antibody, hanga hou complex.AFP taumata he pai taurite ki te tohu fluorescence, me te kukū o AFP i roto i te tauira ka kitea e te fluorescence immunoassay assay.

Te Tikanga Whakamatau

Tena koa panuihia te pukapuka mahi taputapu me te whakauru mokihi i mua i te whakamatautau.

1. Whakatakotoria nga reagents me nga tauira katoa ki te taha mahana.
2. Whakatuwheratia te Portable Immune Analyzer(WIZ-A101), tomo i te takiuru kupuhipa pūkete kia rite ki te tikanga whakahaere o te taputapu, ka uru ki te atanga kitenga.
3. Matawaihia te tohu tohu hei whakau i te mea whakamatautau.
4. Tangohia te kaari whakamatautau mai i te putea pepa.
5. Whakauruhia te kaari whakamatautau ki roto i te mokamoka kaari, matawai i te waehere QR, ka whakatau i te mea whakamatautau.
6. Tāpirihia he 20μL serum, tauira plasma ranei ki roto i te tauira waimeha, ka uru pai.
7. Tāpirihia he otinga tauira 80μL hei tauira pai o te kaari.
8. Patohia te paatene "whakamatautau paerewa", i muri i nga meneti 15, ka kitea aunoa e te taputapu te kaari whakamatautau, ka taea e ia te panui i nga hua mai i te mata whakaatu o te taputapu, me te tuhi / tuhi i nga hua whakamatautau.
9. Tirohia te tohutohu a Portable Immune Analyzer(WIZ-A101).