Ụkpụrụ

The warara nwere mgbochi cal mkpuchi McAb na ule mpaghara na ewu mgbochi oke bekee IgG mgbochi na mpaghara njikwa, nke na-agbakwunyere na akpụkpọ ahụ chromatography tupu oge eruo. Ejiri ọla edo colloidal nke akpọrọ anti cal McAb na kolloidal ọla edo akpọrọ oke bekee IgG mgbochi tupu oge eruo. Mgbe a na-anwale nlele dị mma, cal dị na sample na-abịa na ọla edo colloidal nke akpọrọ anti cal McAb, wee mepụta mgbagwoju anya na-alụso ọrịa ọgụ, ebe a na-ahapụ ya ka ọ kwaga n'akụkụ ebe nnwale ahụ, ihe mgbochi cal conjugate na-ejide McAb na akpụkpọ ahụ na ụdị. Mgbakwụnye "mgbochi cal McAb-cal-colloidal gold nke akpọrọ anti cal McAb" mgbagwoju, eriri ule nwere agba pụtara na mpaghara ule. Agba agba na-ejikọta nke ọma na ọdịnaya cal. Ihe nlele na-adịghị mma anaghị ewepụta eriri ule n'ihi enweghị mgbagwoju anya colloidal gold conjugate cal. N'agbanyeghị cal dị na sample ma ọ bụ na ọ bụghị, enwere eriri uhie na-apụta na mpaghara ntụaka na mpaghara njikwa mma, nke a na-ahụta dị ka ụkpụrụ ụlọ ọrụ dị mma.

Usoro nnwale:

1.Wepụ osisi sampling, tinye n'ime ihe nlele nke nsị, wee tinye osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, megharịa ihe ahụ ugboro 3. Ma ọ bụ na-eji osisi sampling were ihe dị ka 50mg faeces sample, na-etinye n'ime a feces sample tube nwere sample dilution, na ịghasa tightly.

2. Jiri sampling pipette nke a na-atụfu ihe were were ihe nlele ahụ dị gịrịgịrị site n'aka onye ọrịa afọ ọsịsa, tinyezie mmiri 3 (ihe dị ka 100uL) na tube sampling fecal wee maa jijiji nke ọma, wepụ ya.

3.Wepụ kaadị ule ahụ site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.

4.Wepụ okpu site na tube sample na-atụfu akpa abụọ tụlee diluted sample, tinye 4.drops (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.

5.N'ihi kwesịrị ịgụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.