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Ngwa nyocha(Colloidal Gold)maka Hormone na-akpali follicle
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji

A na-eji ngwa ahụ maka nchọpụta qualitative nke ọkwa follicle-stimulating hormone (FSH) na nlele mmamịrị. Ọ dabara maka inye aka ikpebi ọdịdị nke nwanyị menopause.

Nkwakọ ngwaahịa

1 ngwa / igbe, 10 ngwa / igbe, 25 ngwa, / igbe, 50 ngwa / igbe.

Nchịkọta

FSH bụ hormone glycoprotein zoro ezo site na pituitary gland, ọ nwere ike ịbanye n'ọbara na mmamịrị site na mgbasa ọbara. Maka nwoke, FSH na-akwalite ntozu oke nke tubule seminiferous testicular na mmepụta nke spam, maka nwanyị, FSH na-akwalite mmepe follicular na ntozu oke, na-emekọkwata LH ka mkpụrụ ndụ tozuru etozu na-ezobe estrogen na ovulation, na-etinye aka na nhazi nke ịhụ nsọ [1]. FSH na-edobe ọkwa basal kwụsiri ike na isiokwu nkịtị, ihe dịka 5-20mIU/ml. Menopause nke nwanyị na-emekarị n'etiti afọ 49 na 54, na-adịru nkezi nke afọ anọ ruo ise. N'ime oge a, n'ihi atrophy ovarian, follicular atresia na degeneration, estrogen secretion belatara nke ukwuu, ọnụ ọgụgụ buru ibu nke pituitary gonadotropin secretion na-akpali akpali, karịsịa FSH ọkwa ga-abụ nke ukwuu mụbara, bụ n'ozuzu 40-200mIU / ml, na ịnọgide na-enwe larịị na a. ogologo oge^[2]. Ngwa a dabere na teknụzụ nyocha nyocha nke chromatography nke colloidal ọla edo maka nchọpụta qualitative nke FSH antigen n'ụdị mmamịrị mmadụ, nke nwere ike inye nsonaazụ n'ime nkeji iri na ise.

Usoro ASsay
1.Wepụ kaadị ule site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.
2.Discard akpa abụọ tụlee sample, tinye 3 tụlee (banyere 100μL) ọ dịghị afụ sample vertically na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.
3.N'ihi ga-agụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.