Anyị ga-etinye onwe anyị n'inye ndị na-azụ ahịa anyị na-akwanyere ùgwù na-eji ọrụ ndị nwere ịnụ ọkụ n'obi na-eche echiche maka ọnụahịa QuotedNnwale China LhStrip Hot Sale Disposable Lh Ovulation Test Midstream, Anyị ji ezi obi na-eme ike anyị niile iji nye ọrụ kacha mma maka ndị ahịa na ndị ọchụnta ego niile.
Anyị ga-etinye onwe anyị n'inye ndị na-azụ ahịa anyị na-akwanyere ùgwù na-eji ọrụ ndị nwere ịnụ ọkụ n'obi na-eche echiche makaNnwale China Lh, Ule ule, Anyị ụlọ ọrụ ga-rube isi na "Quality mbụ, , izu okè ruo mgbe ebighị ebi, ndị mmadụ-gbakwasara , technology ọhụrụ"Business philosophy. Ọrụ siri ike iji nọgide na-enwe ọganihu, ihe ọhụrụ na ụlọ ọrụ ahụ, na-eme mgbalị ọ bụla na ụlọ ọrụ nke mbụ. Anyị na-agbalị ike anyị niile iji wuo ndị ọkà mmụta sayensị management nlereanya, ịmụta ukwuu ọkachamara ihe ọmụma, ịzụlite elu mmepụta ngwá na mmepụta usoro , na-emepụta mbụ-akpọ àgwà ngwaahịa na ngwọta, ezi uche price , elu àgwà ọrụ , ngwa ngwa nnyefe , inye ị na-emepụta uru ọhụrụ .
Ngwa nyocha maka Alpha-fetoprotein(immunochromatographic nyocha nke fluorescence)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji

Nchọpụta nchọpụta maka Alpha-fetoprotein (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka nchọpụta quantitative nke Alpha-fetoprotein (AFP) na ọbara mmadụ ma ọ bụ plasma, nke a na-ejikarị eme nchọpụta inyeaka, mmetụta ọgwụgwọ na prognosis nke ọrịa cancer hepatocellular bụ isi. A ga-akwadorịrị nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta

Alfa-fetoprotein (AFP) bụ otu n'ime ihe nrịbama akpụ a na-ejikarị eme ihe. Ọ bụ glycoprotein nwere ogo molekụla nke 70,000 na shuga nke 4%.Ọ bụ imeju nwa ebu n'afọ na-emepụta ya, na-esote akwa nkochi ime akwa. 6 izu, na-eru a ọnụ ọgụgụ kasị elu nke 12 ka 15 izu, serum ịta nke 1 ka 3 g/L, na ọbara eriri afọ mgbe a mụrụ 10 ka 100 mg / L; 1 ruo 2 afọ mgbe amuchara nwa ruo okenye; Ime nkịtị nwere ike iru. 90 ruo 500 ng / mL n'etiti; Ọdịnaya nke mmadụ nkịtị nke AFP dị n'etiti 2 na 8 ng / mL, mana ọtụtụ ọrịa, karịsịa ịba ọcha n'anya, na-emetụta uru AFP.

Ụkpụrụ nke usoro

Ekpuchikwa akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi AFP na mpaghara nnwale yana mgbochi ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ antibody AFP na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, antigen AFP dị na nlele na-ejikọta ya na fluorescence akpọrọ antibody AFP, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara ya na mgbochi AFP mkpuchi mgbochi, na-emepụta ọhụrụ complex.AFP larịị na-ọma correlated na fluorescence mgbaàmà, na ịta nke AFP. na sample nwere ike ịchọpụta site fluorescence immunoassay assay.

Reagent na ihe ndị e nyere

Ngwa ngwugwu 25T:

.Kaadị ule n'otu n'otu n'otu n'otu foil nke e ji 25T desiccant tinye ya
.Sample diluents 25T
Ihe ngwungwu 1

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge
NKWUKWU NA NKWUKWU
1.The samples nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2.According ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.
3.All sample zere ifriizi-thaw cycles.

Usoro ASsay
Biko gụọ akwụkwọ ntuziaka ọrụ akụrụngwa na ntinye ngwugwu tupu ịnwale.
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
4.Wepụ kaadị ule site na akpa foil.
5.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6.Add 20μL serum ma ọ bụ plasma sample ka sample diluent, na mix ọma ..
7.Add 80μL sample ngwọta ka ịlele nke ọma nke kaadị.
8.Click bọtịnụ "ọkọlọtọ ule", mgbe nkeji iri na ise gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike ịgụ ihe si na ihuenyo ngosi nke ngwá ọrụ ahụ, ma dekọọ / bipụta nsonaazụ ule.
9. Rụtụ aka na ntuziaka nke Portable Immune Analyzer(WIZ-A101).

Ụkpụrụ echere

AFP: 10ng/ml
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya na-anọchite anya ọnụ ọgụgụ ndị ọrịa ya.

Nsonaazụ ule na ntụgharị asụsụ
.The n'elu data bụ n'ihi nke AFP reagent ule, na ọ na-atụ aro na onye ọ bụla laabu kwesịrị guzobe a nso nke AFP nchọpụta ụkpụrụ kwesịrị ekwesị maka ndị bi na mpaghara a. Nsonaazụ dị n'elu bụ naanị maka ntụnye aka.
.Nsonaazụ nke usoro a na-emetụta naanị na nsonye ntụaka nke e guzobere na usoro a, ọ dịghịkwa ihe jikọrọ ya na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ime ka njehie na nsonaazụ nchọpụta, gụnyere ihe kpatara teknụzụ, njehie arụmọrụ na ihe nlele ndị ọzọ.

Nchekwa na Nkwụsi ike
1.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
2.Do not emepe mechiri emechi obere akpa ruo mgbe ị dị njikere ịrụ a ule, na otu-eji ule na-atụ aro na-eji n'okpuru chọrọ gburugburu ebe obibi (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. dị ka o kwere mee.
3.Sample diluent na-eji ozugbo emeghere ya.

Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​​​na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
. Ọ bụghị mgbanwe reagents n'etiti ngwa nwere dị iche iche nza No.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.

LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké eme ihe, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ụdị. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka nyocha ọbara na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.

ÀJỤMỤ ỌRỤ

REFERENCES
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.

Igodo akara akara ejiri:

	Na Vitro Diagnostic Medical Device
	Onye nrụpụta
	Na-echekwa na 2-30 ℃
	Ụbọchị mmebi
	Ejighachila ya
	kpachara anya
	Kpọtụrụ ntuziaka maka ojiji

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