Ụkpụrụ

The warara nwere MP-Ag mkpuchi antigen na ule mpaghara na ewu mgbochi òké IgG mgbochi na mpaghara akara, nke na-arapara n'ahụ chromatography n'ihu. A na-eji ọla edo colloidal akpọrọ òké-emegide mmadụ IgM McAb kpuchie ya n'ihu. Mgbe a na-anwale nlele dị mma, MP-IgM dị na nlele na-ejikọta ya na ọla edo colloidal akpọrọ òké-emegide mmadụ IgM McAb, wee mepụta mgbagwoju anya. N'okpuru ọrụ nke immunochromatography, mgbagwoju na sample n'ime nitrocellulose membrane na-eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara ya na MP-Ag mkpuchi antigen, na-akpụ "MP-Ag mkpuchi antigen-MP. -IgM-colloidal ọla edo akpọrọ òké-mgbochi mmadụ IgM McAb” mgbagwoju, otu agba ule nwere agba pụtara na mpaghara ule. Ihe nlere na-adịghị mma anaghị ewepụta eriri ule n'ihi ụkọ mgbochi ọrịa. N'agbanyeghị MP-IgM dị na sample ma ọ bụ na ọ bụghị, enwere eriri uhie na-apụta na mpaghara njikwa mma, nke a na-ahụta dị ka ụkpụrụ ụlọ ọrụ dị mma.

Usoro nnwale:

Usoro ule WIZ-A101 hụ ntuziaka nke nyocha ihe mgbochi mgbochi. Usoro nlele anya bụ nke a:

1. Debe ihe niile reagents na sample na ụlọ okpomọkụ.
2. Wepụ kaadị ule site na akpa foil, tinye ya na tebụl dị larịị ma kaa ya akara.
3. Tinye 10μL serum ma ọ bụ plasma sample ma ọ bụ 20μL dum ọbara sample ka sample nke ọma nke kaadị na nyere dispette, wee tinye 100μL (banyere 2-3 drop) sample diluent, malite oge.
4. Chere maka opekempe 10-15 nkeji ma gụọ nsonaazụ, nsonaazụ adịghị mma mgbe nkeji iri na ise gachara.