Ụkpụrụ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na Group A na adenovirus antigen na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad lable site na fluorescence akpọrọ anti Group A na adenovirus na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma maka Group A na adenovirus, otu A na adenovirus dị na sample jikọtara ya na fluorescence akpọrọ anti Rotavirus Group A na adenovirus, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ. Mgbe mgbagwoju gafere mpaghara ule, ọ jikọtara ya na mgbochi Rotavirus Group A na adenovirus mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. Ọ bụrụ na ọ na-adịghị mma, ọ dịghị Rotavirus Group A na adenovirus antigen n'ime ihe nlele ahụ, nke mere na enweghi ike ịmepụta mgbagwoju anya, ọ gaghị enwe eriri uhie na mpaghara nchọpụta (T). N'agbanyeghị ma otu A rotavirus na adenovirus dị na ụdịdị ahụ, a na-edobe òké akara latex IgG na mpaghara njikwa mma (C) wee weghara ya site na mgbochi mgbochi òké IgG. Ahịrị uhie ga-apụta na mpaghara njikwa mma (C). Ahịrị uhie bụ ọkọlọtọ na-egosi na mpaghara njikwa mma (C) maka ikpebi ma enwere ihe nlele zuru oke yana ma usoro chromatography ọ bụ nkịtị. A na-ejikwa ya dị ka ụkpụrụ njikwa ime maka reagents.

Usoro nnwale:

1. Mepee okpu nke tube nchịkọta ihe atụ. Awụpụla ihe ngwọta na karama.
2. Wepụ osisi nlele ahụ, tinye n'ime ihe nlele ahụ (ma ọ bụ jiri osisi sampling were were ihe dị ka 50mg nke nsị), wee tinye osisi nlele ahụ azụ, kpochie ya ma maa jijiji nke ọma, megharịa ihe ahụ ugboro atọ. Were akụkụ dị iche iche nke nsị anụ ahụ oge ọ bụla. Mgbe emechara nlele, tinye mkpanaka nlele n'ime tube mkpokọ nsị nke nwere ihe nlere anya, ma kpochie dropper ahụ nke ọma. Ọ bụrụ na nsị nke onye ọrịa nwere afọ ọsịsa dị gịrịgịrị, enwere ike iji ahịhịa rọba nke a na-atụfu n'efu maka nlele. Iji sample pipette atụfuo, were ihe dị gịrịgịrị sampes si n'aka onye ọrịa afọ ọsịsa, tinyezie 3 tụlee (ihe dị ka 100uL) na fecal sampling tube.
3. Ghichaa ihe nlele ahụ nke ọma ma wepụ okpu n'ọnụ ọnụ dropper wee wepụ ya.
4. Mgbe echekwara na obere okpomọkụ, a ga-eweghachi ngwa ahụ n'ime ụlọ okpomọkụ tupu ejiri ya. Wepụ kaadị ule ahụ na akpa foil, tinye ya na tebụl dị larịị ma kaa ya akara.
5. Wepụ okpu si sample tube na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample kwụ ọtọ na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.
6. A ga-agụ nsonaazụ ya n'ime nkeji 10-15, ọ abaghị uru mgbe nkeji iri na ise gachara.