Ụkpụrụ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi Hp-Ab na mpaghara ule yana mgbochi ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi Hp-Ag na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, Hp-Ab dị na nlele na-ejikọta ya na fluorescence akpọrọ anti Hp-Ag, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ. Mgbe mgbagwoju gafere mpaghara ule, ọ jikọtara ya na mgbochi Hp-Ag mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. Ọ bụrụ na ọ dị njọ, ọ nweghị ihe mgbochi HP na nlele ahụ, nke mere na enweghị ike ịmepụta ogige mgbochi, ọ gaghị enwe ahịrị uhie na mpaghara nchọpụta (T). Ahịrị uhie bụ ọkọlọtọ na-egosi na mpaghara njikwa mma (C) maka ikpebi ma enwere ihe nlele zuru oke yana ma usoro chromatography ọ bụ nkịtị. A na-ejikwa ya dị ka ụkpụrụ njikwa ime maka reagents.

Usoro nnwale:

Biko gụọ ihe ntinye ngwugwu tupu ịnwale.

1. Wepụ kaadị ule ahụ na akpa foil, tinye ya na tebụl dị larịị ma kaa ya akara.

2. Tinye 2 tụlee nke serum ma ọ bụ plasma sample (ma ọ bụ 3 tụlee ọbara dum / mkpịsị aka) ka ịlele nke ọma nke kaadị na nyere dispette, wee tinye 1 drop of sample diluent, malite oge.

3. Chere opekempe 10-15 nkeji ma gụọ nsonaazụ na nkeji 10-15. Nsonaazụ abaghị uru ka nkeji iri na ise gachara.