Nchịkọta

Vitamin D bụ vitamin ma bụrụkwa homonụ steroid, nke gụnyere VD2 na VD3, nke usoro ha yiri nnọọ. Vitamin D3 na D2 na-atụgharị gaa na 25 hydroxyl vitamin D (gụnyere 25-dihydroxyl vitamin D3 na D2). 25- (OH) VD n'ime ahụ mmadụ, nkwụsi ike struction, elu itinye uche. 25- (OH) VD na-egosipụta ọnụọgụ vitamin D zuru oke, na ikike ngbanwe nke vitamin D, ya mere 25- (OH) VD na-ewere dị ka ihe ngosi kachasị mma maka ịlele ọkwa vitamin D. Ihe nchọpụta nchọpụta dabeere na ya. Immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na conjugate nke BSA na 25- (OH) VD na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence anti 25-(OH) VD mgbochi na oke bekee IgG tupu oge eruo. Mgbe a na-anwale sample, 25- (OH) VD na sample jikọtara na fluorescence akara mgbochi 25- (OH) VD mgbochi, na-etolite dịghịzi ngwakọta. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent akara ga-ejikọta na 25- (OH) VD na akpụkpọ ahụ. The ịta nke 25- (OH). VD bụ ihe na-adịghị mma maka mgbaàmà fluorescence, yana ntinye nke 25- (OH) VD na nlele nwere ike chọpụta site na fluorescence immunoassay assay.