Prinsipul

Musinody nke ngwaọrụ nnwale na mpaghara nnwale na goat mgbochi oke bekee na-egbu egbu na mpaghara njikwa. A na-ejikọ ụgbọ mmiri site na fluoorcence na-ede akara na-ahazi mgbochi na oke bekee na-aga n'ihu. Mgbe a na-anwale ezigbo nlele, antin71 antigen na nlele na fluorelence akpọrọ anti ev71 Antody, na-emekọrịta ngwakọta. N'okpuru ihe nke Chromatography, mgbagwoju anya dị na ntuziaka na-atọ ụtọ, mgbe ọ na-agagharị na mpaghara ule, ya na Anvi71 Coanish Anlibod, na-etolite ogige ọhụrụ.

Ọ bụrụ na ọ bụ ihe na-adịghị mma, ihe nlele ahụ enweghị Tatavirus 71 Igm Antod, nke mere na enweghị ike ịkpụzi ya. Agaghị enwe akara uhie na mpaghara nchọpụta (t). N'agbanyeghi ma Stateovirus 71 IGM Antody dị na ụdị ma ọ bụ na - egbochi collonal ọla edo na - emegide ụmụ Mouse IGG Ancu-mose IGG Angubody Corated mpaghara (c). Mgbe ahụ, agglutinates na-etolite agba na mpaghara njikwa dị mma, yana akara uhie ga-apụta na (c). Line na-acha uhie uhie bụ ọkọlọtọ na-egosi na mpaghara njikwa dị mma (c) maka ikpe ma enwere ụfọdụ ihe ndị zuru ezu na usoro chromatom bụ ihe dị mma. A na-ejikwa ya dị ka usoro nchịkwa dị n'ime.

Usoro nnwale:

1.Ma nlele anwalere nwere ike ịbụ ọbara zuru oke, gụnyere ọbara dị ala ma ọ bụ ọbara. A gaghị echekwa ọbara mgbe anakọtara. Ekwesịrị m iji ya na-achịkọta.

A na-anakọta ihe nlere 2.Sum Adoptical nke ọma dịka usoro ọkọlọtọ. Ọkpụkpụ ọkụ na-enweghị isi enweghị ike iji. A naghị atụ aro iji lipe liperimi, turdid ma ọ bụ ọbara. Ngụkọta mkparị na ọbara. Na mbufe ga - emetụta nsonaazụ ule ahụ, ihe nlere ndị ahụ kwesịrị ịdị centrifugud ma ọ bụ ẹdụk tupu ojiji.

3.Ọ bụ sample nwalere nwere ike ịbụ Hepatin, sodium glutate ma ọ bụ Edta anticoulant plasma.

4. According na usoro ọkọlọtọ na-anakọta ihe nlele. Enwere ike idowe ọnya ma ọ bụ plasma nlegharị anya na 2-8 ℃ ruo ụbọchị 3 na prictoproventation dị n'okpuru -15 Celsius C ruo ọnwa 3.

5. Ilele Sale na-ezere crycles.