Ụkpụrụ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na FOB antibody na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi FOB antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, FOB antigen dị na sample na-ejikọta ya na fluorescence akpọrọ mgbochi FOB, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ. Mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara ya na mgbochi FOB mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. Ọ bụrụ na ọ dị njọ, ihe nlele ahụ enweghị haemoglobin mmadụ ma ọ bụ ọdịnaya dị ala, enweghị ike ịmepụta ogige mgbochi zuru oke. Agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). Ahịrị uhie bụ ọkọlọtọ na-egosi na mpaghara njikwa mma (C) maka ikpebi ma enwere ihe nlele zuru oke yana ma usoro chromatography ọ bụ nkịtị. A na-ejikwa ya dị ka ụkpụrụ njikwa ime maka reagents.

Usoro nnwale:

Biko gụọ ihe ntinye ngwugwu tupu ịnwale.

1.Wepụ kaadị ule site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.

2.Wepụ okpu si sample tube na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample kwụ ọtọ na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette. Mgbe ahụ malite ngụ oge.

3.N'ihi ga-agụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.