Nchọpụta Kit maka ngụkọta nke ngụkọta?(fluorescence immunoatographic)
Maka na vitro divostic na-eji naanị
Biko gụọ ngwugwu a tinye nke ọma tupu oge iji ma sorosie ntuziaka a. Enweghị ntụkwasị obi nke nsonaazụ arụpụtara enweghị ike ikwenye ma ọ bụrụ na enwere nrụpụta ọ bụla sitere na ntuziaka na ngwugwu a.

Eji eji
Nchọpụta Kit maka ngụkọta ihe mgbochi nke ihe omume (nke na-emegharị ihe omume nke ihe omume nke otu) Ekwesịrị ikwenye site na usoro ndị ọzọ. Ezubere ule a maka iji ọrụ ọkachamara ahụike.

Nchikota
Trioodothyronine (T3) molecular arọ 651d. Ọ bụ ụdị arụ ọrụ nke homonụ thyroid. Ngụkọta T3 (ngụkọta T3, tt3) na serm kewapụrụ iche na ụdị free. 99.5% nke TT3 na-eme ka ihe owuwu na-ejikọ protein (TBP), na f3 (na-akwụghị ụgwọ T3) maka 0.2 ruo 0.4%. T4 na T3 sonyere na idozi ma na-ahazi usoro ọrụ metabolic ahụ.Tt3 iji nyochaa ọnọdụ ọrụ thyroid arụmọrụ na nchoputa nke ọrịa. Clinical TTT3 bụ ihe ngosi a pụrụ ịdabere na ya maka nchọpụta na arụmọrụ arụmọrụ nke hyperthyroidism na hypothyroidism.The mkpebi nke T3 dị mkpa maka nchọpụta nke hypethyroids karịa T4.

Ụkpụrụ nke usoro

Thembrane nke ngwaọrụ nnwale a bụ ihe mkpuchi nke BSA na T3 na Mpaghara nnwale na ewu oke bekee na-egbu egbu na mpaghara njikwa. A na-eji akara ngosi na-ekpo ọkụ na-achị achị Market T3 Antinody na oke bekee na-aga n'ihu. Mgbe ịnwale nlele, TT3 na nlele jikọtara ya na fluorescence akara mgbochi mgbochi, na-arụ ọrụ na ngwakọta. N'okpuru ime amumumomigraphromatographyromatoma, mgbagwoju anya na ntuziaka na-agakọ, ọ ga-ejikọ akara ule ahụ na T3 na TT3 bụ ihe na-adịghị mma maka ihe mgbaàmà na-adịghị mma A na-ahụta nke TT3 na nlele nwere ike ịchọpụta ihe mgbochi immuniassay.

Nta na ihe enyere

Ihe ngwungwu nke 25t:
.Test kaadị n'otu n'otu na-akpachapụrụ ya na 25t
.A ngwọta 25t
.B ngwọta 1
.Cage tinye 1

Ihe achọrọ mana enyeghị ya
Nzacha nchịkọta, oge

Nchịkọta nlele na nchekwa
1. Eserese na-enyocha nwere ike ịbụ n'ọbara, Hepatin anticoulantulant plasma ma ọ bụ Edta anticoulant plasma.
2.Ccording na usoro ọkọlọtọ na-anakọta nlele. Enwere ike idowe ọnya ma ọ bụ plasma nlegharị na 2-8 ℃ maka 7days na pricreroves dị n'okpuru -15 Celsius C ruo ọnwa isii.
3. Samall Sale na-ezere crycles na-akwụ ụgwọ.

Usoro nkwenye
Usoro nnwale nke ngwa ahụ na-ahụ akwụkwọ ntuziaka immunonalyher. Usoro nyocha ahụ dị ka ndị a
1.Lay wezuga onwe-ya na ihe nlere rue ulo okpomọkụ.
2.pen onye nyocha a na-etinyeghị ike (Wiz-A101), tinye paswọọdụ ndekọ akaụntụ dị ka usoro arụmọrụ nke ngwa ahụ, wee tinye ihe nchọpụta ahụ.
3.scan koodu ezé iji gosipụta ihe nnwale.
4.Nata kaadị nnwale ahụ site na akpa foil.
Kechie kaadị nnwale ahụ n'ime oghere kaadị ahụ, nyochaa koodu QR, ma kpebie ihe nnwale ahụ.
6.Add 30μL Serm ma ọ bụ ihe nlele plasma ka ọ bụrụ ihe ngwọta, ma gwakọta nke ọma.
7.DD 20μL B Ngwọta na ngwakọta a dị n'elu, ma gwakọta nke ọma.
8.Lapachaa ngwakọta ahụ ruo minit 20.
9.0cad 80μL ngwakọta na grale nke ọma nke kaadị a.
3. Pịa bọtịnụ "Elekere ọkọlọtọ", mgbe minit 10 gachara, ngwa ahụ achọpụtala kaadị nnwale ahụ, ọ nwere ike gụọ nsonaazụ ya site na ihuenyo ngosipụta ya, ma detuo / Binye nsonaazụ ule.
11.aref na ntuziaka nke onye nyocha nke onye nyocha nke onye nyocha nke onye nyocha nke a na - ebuso ya (Wiz-A101).

Ụkpụrụ a tụrụ anya ya

Tt3 nkịtị: 0,5-2.5ng / ml
A na-atụ aro ka ụlọ nyocha ọ bụla guzobere ụdị ndị ọ na-anọchi anya ya.

Nsonaazụ nnwale na ịkọwa
.Ndị data dị n'elu bụ ntinye akwụkwọ nyocha nke ngwa ahịa a, a na-atụkwa aro na laabu na laabu nke ndi bi na mpaghara a di nkpa.
.Ndị na TT3 dị elu karịa oke ntinye aka, yana mmeghachi omume physiological ma ọ bụ mmeghachi omume zuru oke, ekwesịrị ijikọta ọgwụ mgbochi ọrịa.
.Ndị nsonaazụ a bụ naanị ntinye aka nke usoro a, nsonaazụ ya adịghị atụnyere usoro ndị ọzọ.
.

Nchekwa na nkwụsi ike
1.The Kit bụ ọnwa 18 na ndụ site na ụbọchị imepụta. Chekwaa Kits a na-ejighị na 2-30 Celsius C. Emela ihe. Ejila ụbọchị mmebi.
2.De emepeghị obere akpa ahụ ruo mgbe ị dịla njikere ịme ule, a na-atụ aro ka a na-ahụ maka otu ihe n'okpuru gburugburu chọrọ (okpomọkụ 40-90%) n'ime nkeji 60 ka enwere ike.
A na-eji dilute 3.sample dị ngwa mgbe a mepere ya.

Warningsdọ aka na ntị na akpachapụ anya
.The Kit ga-akara ma chekwaa ya pụọ ​​na mmiri.
.
.
.Da anaghị eji reserd resergent.
.Dlie abụghị ngwa ahịa n'etiti Kits na adịghị iche iche ..
.Na na-ejikwa kaadị ule na ngwa ọ bụla.
.Miche, oke nlele ma ọ bụ obere ihe nlele nwere ike ibute nsonaazụ.

LNnomi
.As na ihe ndị ọ bụla na-eme ihe na-eme ihe na-eme ihe na-arụ ọrụ, o nwere ike ime ihe mgbochi site na mgbochi mgbochi nke ụmụ mmadụ (HAMA) na ụdị. Specisens sitere n'aka ndị ọrịa natara nkwadebe nke nje mgbochi monoconosis ma ọ bụ ọgwụgwọ nwere ike ịnwe Hama. Ụdị ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ ụgha.
.Ndị nnwale ahụ bụ naanị maka ntụle ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe kpatara ya, nyocha ahụike, nzaghachi, mmeghachi omume, EpideMology na ozi ndị ọzọ .
.HIGHIGHIRSASIRSA BITERRURU EGO NA-EGO NA MARA EGO. Ọ nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dị ka mmiri na mmamịrị na wdg.

Njirimara arụmọrụ

REnerise
1.Hassan JH, et Ntinye na ọgwụ ọjọọ nke sitere na immunody nke sitere na immunody [j].
2.levinson ss.The ọdịdị nke antibodic na intermomilic na intermomiasy miner (j] .j nke immunoasy, 1992: 108-114.

Igodo iji gosiputa ihe:

	Na vitronic Victostic ngwaọrụ
	Mmewe
	Chekwaa na 2-30 ℃
	Ụbọchị mmebi
	Etula ya
	Ikpachara anya
	Pụọ ntuziaka maka iji

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