Ngwa nyocha maka mkpokọta Thyroxine (immunochromatographic nyocha nke fluorescence)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji
Kit diagnostic for Total Thyroxine (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka quantitative nchọpụta nke Total Thyroxine (TT4) na mmadụ serum ma ọ bụ plasma, nke a na-ejikarị enyocha ọrụ thyroid. Ọ bụ ihe inyeaka reagent.All nti sample sample. a ga-akwadorịrị site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta
A na-ezobe Thyroxine (T4) site na gland thyroid na ịdị arọ ya bụ 777D. Ngụkọta T4 (Total T4,TT4) n'ime ọbara bụ ugboro 50 karịa nke ọbara T3. N'ime ha, 99.9% nke TT4 na-ejikọta na serum Thyroxine Binding Proteins (TBP), na T4 (Free T4, FT4) bụ ihe na-erughị 0.05%. T4 na T3 na-ekere òkè n'ịhazi ọrụ metabolic nke anụ ahụ. A na-eji nha TT4 iji nyochaa ọnọdụ ọrụ thyroid na nyocha nke ọrịa. N'ụlọ ọgwụ, TT4 bụ ihe ngosi a pụrụ ịdabere na ya maka nyocha na nyocha arụmọrụ nke hyperthyroidism na hypothyroidism.

Ụkpụrụ nke usoro
A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na conjugate nke BSA na T4 na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence antibody T4 na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele, TT4 na sample jikọtara ya na fluorescence akara mgbochi mgbochi T4, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju mgbagwoju anya na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent marker ga-ejikọta T4 na akpụkpọ ahụ. The ịta nke TT4 bụ ọjọọ mmekọrịta maka fluorescence mgbaàmà, na na Enwere ike ịchọpụta mkpokọta TT4 na nlele site na nyocha immunoassay fluorescence.

Reagent na ihe ndị e nyere

Ngwa ngwugwu 25T:
.Kaadị ule n'otu n'otu n'otu n'otu foil nke e ji 25T desiccant tinye ya
.A ngwọta 25T
.B ngwọta 1
Ihe ngwungwu 1

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge

NKWUKWU NA NKWUKWU
1.The samples nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2.According ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.
3.All sample zere ifriizi-thaw cycles.

Usoro ASsay

Usoro ule nke ngwa ahụ hụ akwụkwọ ntuziaka immunoanalyzer. Usoro ule reagent bụ nke a
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
3.Wepụ kaadị ule site na akpa foil.
4.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
5.Tinye 20μL serum ma ọ bụ plasma sample na A ngwọta, na mix ọma.
6.Tinye 20μL B ngwọta na ngwakọta n'elu, ma jikọta nke ọma.

Hapụ ngwakọta maka20nkeji.
Tinye ngwakọta 80μL ka ọ bụrụ nlele nke ọma nke kaadị ahụ.
Pịa bọtịnụ “ọkọlọtọ ule”, mgbe nkeji 10 gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike gụọ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, wee dekọọ / bipụta nsonaazụ ule.
Rụtụ aka na ntuziaka Portable Immune Analyzer(WIZ-A101).

Ụkpụrụ echere
TT4 nkịtị nso: 55-140nmol/L
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya na-anọchite anya ọnụ ọgụgụ ndị ọrịa ya.

Nsonaazụ ule na ntụgharị asụsụ
.Ihe data dị n'elu bụ oge ntinye aka na-eguzobe maka nchọpụta nchọpụta nke kit a, a na-atụkwa aro na ụlọ nyocha ọ bụla kwesịrị ịmepụta oge ntinye aka maka mkpa ụlọ ọgwụ dị mkpa nke ndị bi na mpaghara a.
.Nkwadebe nke TT4 dị elu karịa ebe a na-ekwu okwu, na mgbanwe physiological ma ọ bụ nzaghachi nrụgide kwesịrị iwepụ. N'ezie ihe na-adịghị mma, kwesịrị ijikọta nchọpụta mgbaàmà ahụike.
.Nsonaazụ nke usoro a na-emetụta naanị na nsonye ntụaka nke usoro a guzobere, na nsonaazụ adịghị atụnyere ya na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ime ka njehie na nsonaazụ nchọpụta, gụnyere ihe kpatara teknụzụ, njehie arụmọrụ na ihe nlele ndị ọzọ.

Nchekwa na Nkwụsi ike
.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
.Emepela obere akpa ahụ mechiri emechi ruo mgbe ị dị njikere ịme ule, a na-atụ aro ka a na-eji ule otu-eji n'okpuru ebe achọrọ (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. kwere omume.
.Sample diluent na-eji ozugbo emeghere.

Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​​​na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
.AKWỤKWỌ reagents n'etiti ngwa na dị iche iche nza mba.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.

LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ime ihe atụ a. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka nyocha ọbara na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.

ÀJỤMỤ ỌRỤ

REFERENCES
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.

Igodo akara akara ejiri:

	Na Vitro Diagnostic Medical Device
	Onye nrụpụta
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	Ụbọchị mmebi
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	kpachara anya
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