Ngwa nyocha maka microalbumin mmamịrị

(Fluorescence Immunochromatographic assay)

N'ihi na in vitro diagnostic ojiji naanị

Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji

Ngwa nyocha maka mmamịrị microalbumin (Fluorescence Immunochromatographic Assay) dabara maka nchọpụta quantitative nke microalbumin na mmamịrị mmadụ site na fluorescence immunochromatographic assay, bụ nke a na-ejikarị maka nyocha inyeaka nke ọrịa akụrụ. A ga-enwerịrị ihe nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta

Microalbumin bụ protein nkịtị a na-achọta n'ọbara ma ọ dị ụkọ na mmamịrị mgbe metabolized na-adịkarị. Ọ bụrụ na enwere ọnụọgụ n'ime mmamịrị Albumin karịa 20 micron / ml, bụ nke microalbumin urinary, ma ọ bụrụ na enwere ike ịgwọ ya n'oge, ọ nwere ike rụkwaa glomeruli kpamkpam, wepụ proteinuria, ma ọ bụrụ na ọ bụghị ọgwụgwọ n'oge, nwere ike ịbanye na usoro uremia. A na-ahụkarị microalbumin urinary na nephropathy na-arịa ọrịa shuga, ọbara mgbali elu na preeclampsia n'afọ ime. Enwere ike ịchọpụta ọnọdụ ahụ n'ụzọ ziri ezi site na uru nke microalbumin urinary, jikọtara ya na omume, mgbaàmà na akụkọ ahụike. Nchọpụta mbụ nke mmamịrị microalbumin dị ezigbo mkpa iji gbochie na igbu oge mmepe nke nephropathy mamịrị.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na ALB antigen na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence antibody ALB na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele, ALB na sample jikọtara ya na fluorescence akara mgbochi mgbochi ALB, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent akara ga-ejikọta na ALB na akpụkpọ ahụ. The ịta nke ALB bụ ọjọọ mmekọrịta maka fluorescence mgbaàmà, na na Enwere ike ịchọpụta mkpokọta ALB na nlele site na nyocha immunoassay fluorescence.

Reagent na ihe ndị e nyere

Ngwa ngwugwu 25T:

Kaadị ule n'otu n'otu ejiri akpa 25T desiccant tinye ya

Ntinye ngwugwu 1

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ

Akpa mkpokọta ihe nlele, ngụ oge

NKWUKWU NA NKWUKWU

1. Ihe nlele a nwalere nwere ike ịbụ mmamịrị.
2. Enwere ike ịnakọta ihe nlele mmamịrị ọhụrụ n'ime akpa dị ọcha enwere ike iwepụ. A na-atụ aro ka ịnwale ụdị mmamịrị ozugbo anakọtara. Ọ bụrụ na enweghị ike ịnwale ụdị mmamịrị ahụ ozugbo, biko chekwaa ya na 2-8℃, ma a na-atụ aro ka ị ghara ịchekwae ha maka ihe karịrị awa 12. Ejila akpa ahụ maa jijiji. Ọ bụrụ na enwere sedimenti na ala nke akpa ahụ, were supernatant maka nnwale.
3. Nlereanya niile na-ezere okirikiri ịsa oyi.
4. Gwaa ihe nlele na ọnụ ụlọ tupu ejiri ya.