Ngwa nyocha makaHormone luteinizingfluorescence immunochromatographic nyocha)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji
Ihe nchọpụta diagnostic maka Luteinizing Hormone (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka quantitative nchọpụta nke Luteinizing Hormone (LH) na mmadụ serum ma ọ bụ plasma, nke a na-ejikarị na nyocha nke pituitary endocrine ọrụ.All nti sample ga-akwado ya site na. usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta

Luteinizing hormone (LH) bụ glycoprotein nwere ihe dị ka 30,000 Dalton dị arọ, nke pituitary na-emepụta na-emepụta. Ntinye uche nke LH nwere njikọ chiri anya na ovulation nke ovaries, na ọnụ ọgụgụ kasị elu nke LH ga-abụ awa 24 ruo 36 nke ovulation. Ya mere, enwere ike nyochaa ọnụ ahịa kachasị elu nke LH n'oge oge ịhụ nsọ iji chọpụta oge kachasị mma. Ọrụ endocrine na-adịghị mma na gland pituitary nwere ike ime ka nzuzo nzuzo LH ghara ịdị irè. Enwere ike iji ntinye uche nke LH nyochaa ọrụ endocrine pituitary. Ngwa nyocha ahụ dabere na immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi LH na mpaghara ule yana mgbochi ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence nke akpọrọ mgbochi LH antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, antigen LH dị na nlele na-ejikọta ya na fluorescence akpọrọ mgbochi LH, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara ya na mgbochi LH mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. A na-ejikọta ọkwa LH nke ọma na mgbama fluorescence, na ntinye nke LH na nlele nwere ike chọpụta site na fluorescence immunoassay assay.

Reagent na ihe ndị e nyere

Ngwa ngwugwu 25T:

.Kaadị ule n'otu n'otu n'otu n'otu foil nke e ji 25T desiccant tinye ya
.Sample diluents
.Ngwunye ntinye

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge

NKWUKWU NA NKWUKWU
1.The samples nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2.According ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.
3.All sample zere ifriizi-thaw cycles.

Usoro ASsay

Usoro ule nke ngwa ahụ hụ akwụkwọ ntuziaka immunoanalyzer. Usoro ule reagent bụ nke a
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
4.Wepụ kaadị ule site na akpa foil.
5.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6.Add 20μL serum ma ọ bụ plasma sample ka sample diluent, na mix ọma ..
7.Add 80μL sample ngwọta ka ịlele nke ọma nke kaadị.
8.Click bọtịnụ "ọkọlọtọ ule", mgbe nkeji iri na ise gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike ịgụ ihe si na ihuenyo ngosi nke ngwá ọrụ ahụ, ma dekọọ / bipụta nsonaazụ ule.
9. Rụtụ aka na ntuziaka nke Portable Immune Analyzer(WIZ-A101).

Nsonaazụ ule na ntụgharị asụsụ

.Ihe data dị n'elu bụ oge ntinye aka na-eguzobe maka nchọpụta nchọpụta nke kit a, a na-atụkwa aro na ụlọ nyocha ọ bụla kwesịrị ịmepụta oge ntinye aka maka mkpa ụlọ ọgwụ dị mkpa nke ndị bi na mpaghara a.
.Ntụkwasị uche nke LH dị elu karịa ebe a na-ekwu okwu, na mgbanwe physiological ma ọ bụ nzaghachi nrụgide kwesịrị iwepụ. N'ezie ihe na-adịghị mma, kwesịrị ijikọta nchọpụta mgbaàmà ahụike.
.Nsonaazụ nke usoro a na-emetụta naanị na nsonye ntụaka nke usoro a guzobere, na nsonaazụ adịghị atụnyere ya na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ime ka njehie na nsonaazụ nchọpụta, gụnyere ihe kpatara teknụzụ, njehie arụmọrụ na ihe nlele ndị ọzọ.

Nchekwa na Nkwụsi ike
1.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
2.Do not emepe mechiri emechi obere akpa ruo mgbe ị dị njikere ịrụ a ule, na otu-eji ule na-atụ aro na-eji n'okpuru chọrọ gburugburu ebe obibi (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. dị ka o kwere mee.
3.Sample diluent na-eji ozugbo emeghere ya.

Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​​​na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
. Ọ bụghị mgbanwe reagents n'etiti ngwa nwere dị iche iche nza No.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.

LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké eme ihe, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ụdị. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka nyocha ọbara na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.

ÀJỤMỤ ỌRỤ

Ntụaka
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.

Igodo akara akara ejiri:

	Na Vitro Diagnostic Medical Device
	Onye nrụpụta
	Na-echekwa na 2-30 ℃
	Ụbọchị mmebi
	Ejighachila ya
	kpachara anya
	Kpọtụrụ ntuziaka maka ojiji

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