Nchọpụta Kit makaÌgwè hormonefluorescence immunorogratogratographic)
Maka na vitro divostic na-eji naanị
Biko gụọ ngwugwu a tinye nke ọma tupu oge iji ma sorosie ntuziaka a. Enweghị ntụkwasị obi nke nsonaazụ arụpụtara enweghị ike ikwenye ma ọ bụrụ na enwere nrụpụta ọ bụla sitere na ntuziaka na ngwugwu a.

Eji eji
Nchọpụta na-achọta ihe mkpuchi na-eme ka ọ bụrụ nke a na-eme ihe nkiri na-enweghị atụ (lh) na-ahụ maka ihe nlere anya nke na-arụ ọrụ. A ga-egosirịrị ihe atụ Uzo ndi ozo. Ezubere ule a maka iji ọrụ ọkachamara ahụike.

Nchikota

Lylone na-acha odo odo (lh) bụ glycoprotein dị arọ nke ihe dị ka 30,000 Dalton, nke na-emepụta pioutio. Thementzụ nke LH nwere njikọ chiri anya na mkpuchi nke ovaries, na ọnụ ọgụgụ kasị elu nke LH awa 24 ruo 36 nke mkpuchi. Ya mere, enwere ike inyocha oke ọnụọgụ LH n'oge usoro nsọ nwanyị iji chọpụta oge ncheta afọ ojuju kachasị. A na-eji ọrụ ndị a na-ahụkarị na Gland Gland nwere ike ime ka ihe nzuzo LH nwere ike ibute ọrụ LH. Kit diagn diagn diagn diagnያ na immunocromatigraphy ma nwee ike ịnye ya n'ime nkeji iri na ise.

Ụkpụrụ nke usoro

Thembrane nke ngwaọrụ nnwale a na-eji Antibdody na mpaghara nnwale ma wuo mgbochi oke bekee IGG Angumpy na mpaghara njikwa. A na-eji akwa igwe na-ebugharị site na fluoorcence depụtara mgbochi na oke bekee iigg tupu oge eruo. Mgbe ị na-anwale ezigbo nlele, antigen na nlele jikọtara na fluorectonce akpọrọ anti LH Ancorod, ma na-arịa ngwa ngwa ngwakọta. N'okpuru ime ihe omume immunochromatoma, mgbagwoju anya na ntuziaka na-atọ ụtọ, mgbe ọ ga-agafe mpaghara ule, ya na Anti Lh Conish Assod, na-etolite ogige ọhụrụ. Lh larịị doro anya na mgbaama nke fluoriocesce, na ịta ahụhụ nwere ike ịchọpụta na ịmịcha ọgwụ mgbochi mmiri.

Nta na ihe enyere

Ihe ngwungwu nke 25t:

.Test kaadị n'otu n'otu na-akpachapụrụ ya na 25t
.
.Ckage Tinye

Ihe achọrọ mana enyeghị ya
Nzacha nchịkọta, oge

Nchịkọta nlele na nchekwa
1. Eserese na-enyocha nwere ike ịbụ n'ọbara, Hepatin anticoulantulant plasma ma ọ bụ Edta anticoulant plasma.
2.Ccording na usoro ọkọlọtọ na-anakọta nlele. Enwere ike idowe ọnya ma ọ bụ plasma nlegharị na 2-8 ℃ maka 7days na pricreroves dị n'okpuru -15 Celsius C ruo ọnwa isii.
3. Samall Sale na-ezere crycles na-akwụ ụgwọ.

Usoro nkwenye

Usoro nnwale nke ngwa ahụ na-ahụ akwụkwọ ntuziaka immunonalyher. Usoro nyocha ahụ dị ka ndị a
1.Lay wezuga onwe-ya na ihe nlere rue ulo okpomọkụ.
2.pen onye nyocha a na-etinyeghị ike (Wiz-A101), tinye paswọọdụ ndekọ akaụntụ dị ka usoro arụmọrụ nke ngwa ahụ, wee tinye ihe nchọpụta ahụ.
3.scan koodu ezé iji gosipụta ihe nnwale.
4.Nata kaadị nnwale ahụ site na akpa foil.
Kechie kaadị nnwale ahụ n'ime oghere kaadị ahụ, nyochaa koodu QR, ma kpebie ihe nnwale ahụ.
6.Add 20μL Serm ma ọ bụ ihe nlele plasma iji mee ihe atụ, ma gwakọta nke ọma ..
7.00μL nlele nlele maka eserese nke kaadị a.
8. Pịa bọtịnụ "Elekere ọkọlọtọ", mgbe minit 15 gasịrị, ngo ahụ chọpụta kaadị nnwale ahụ, ọ nwere ike gụọ nsonaazụ ya site na ihuenyo ngosipụta, na ndekọ / dekọọ ya / Binye nsonaazụ ule.
9.Refer na ntuziaka nke onye nyocha nke onye nyocha nke onye nyocha nke a na-etinye (Wiz-A101).

Nsonaazụ nnwale na ịkọwa

.Ndị data dị n'elu bụ ntinye akwụkwọ nyocha nke ngwa ahịa a, a na-atụkwa aro na laabu na laabu nke ndi bi na mpaghara a di nkpa.
.Ndị ngbanwe nke LH dị elu karịa oke ntinye aka, yana mmeghachi omume physiological ma ọ bụ mmeghachi omume na-efe efe, kwesịrị ijikọta nyocha nyocha.
.Ndị nsonaazụ a bụ naanị ntinye aka nke usoro a, nsonaazụ ya adịghị atụnyere usoro ndị ọzọ.
.

Nchekwa na nkwụsi ike
1.The Kit bụ ọnwa 18 na ndụ site na ụbọchị imepụta. Chekwaa Kits a na-ejighị na 2-30 Celsius C. Emela ihe. Ejila ụbọchị mmebi.
2.De emepeghị obere akpa ahụ ruo mgbe ị dịla njikere ịme ule, a na-atụ aro ka a na-ahụ maka otu ihe n'okpuru gburugburu chọrọ (okpomọkụ 40-90%) n'ime nkeji 60 ka enwere ike.
A na-eji dilute 3.sample dị ngwa mgbe a mepere ya.

Warningsdọ aka na ntị na akpachapụ anya
.The Kit ga-akara ma chekwaa ya pụọ ​​na mmiri.
.
.
.Da anaghị eji reserd resergent.
. Ọ bụghị ngwa ahịa n'etiti Kits na nza adịghị adị ..
.Na na-ejikwa kaadị ule na ngwa ọ bụla.
.Miche, oke nlele ma ọ bụ obere ihe nlele nwere ike ibute nsonaazụ.

LNnomi
.As na ihe ndị ọ bụla na-eme ihe na-eme ihe na-eme ihe na-arụ ọrụ, o nwere ike ime ihe mgbochi site na mgbochi mgbochi nke ụmụ mmadụ (HAMA) na ụdị. Specisens sitere n'aka ndị ọrịa natara nkwadebe nke nje mgbochi monoconosis ma ọ bụ ọgwụgwọ nwere ike ịnwe Hama. Ụdị ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ ụgha.
.Ndị nnwale ahụ bụ naanị maka ntụle ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe kpatara ya, nyocha ahụike, nzaghachi, mmeghachi omume, EpideMology na ozi ndị ọzọ .
.HIGHIGHIRSASIRSA BITERRURU EGO NA-EGO NA MARA EGO. Ọ nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dị ka mmiri na mmamịrị na wdg.

Njirimara arụmọrụ

Ebe zoro aka
1.Hassan JH, et Ntinye na ọgwụ ọjọọ nke sitere na immunody nke sitere na immunody [j].
2.levinson ss.The ọdịdị nke antibodic na intermomilic na intermomiasy miner (j] .j nke immunoasy, 1992: 108-114.

Igodo iji gosiputa ihe:

	Na vitronic Victostic ngwaọrụ
	Mmewe
	Chekwaa na 2-30 ℃
	Ụbọchị mmebi
	Etula ya
	Ikpachara anya
	Pụọ ntuziaka maka iji

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