Ngwa nyocha maka Ọbara mgbaasị(Fluorescence Immunochromatographic assay
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji
Kit diagnostic maka fecal mgbaasị ọbara (Fluorescence Immunochromatographic Assay) kwesịrị ekwesị maka quantitative nchọpụta hemoglobin na ụmụ mmadụ faecs site fluorescence immunochromatographic assay, ọ na-eme dị ka eriri afọ ọbara ọgbụgba auxiliary nchoputa reagent clinical nchoputa. All nti sample ga-akwadoro site na ndị ọzọ methodologies. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta
Obere ọbara ọgbụgba nke ọrịa tract digestive na-ebute FOB, yabụ nchọta FOB nwere uru dị mkpa maka nchọpụta inyeaka nke ọrịa ọbara ọgbụgba nke eriri afọ, enwere ụzọ maka nyocha ọrịa tract digestive.

Ụkpụrụ nke usoro
Iberibe ahụ nwere mgbochi mgbochi FOB mkpuchi na mpaghara nnwale, nke etinyere na chromatography akpụkpọ ahụ tupu oge eruo. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi FOB mgbochi tupu oge eruo. Mgbe a na-anwale nlele dị mma, FOB dị na nlele nwere ike ịgwakọta ya na fluorescence akpọrọ mgbochi FOB, wee mepụta ngwakọta mgbochi. Ka a na-ekwe ka ngwakọta ahụ kwaga n'akụkụ eriri ule ahụ, FOB conjugate complex na-ejide ihe mgbochi mkpuchi FOB na akpụkpọ ahụ wee mepụta mgbagwoju anya. A na-ejikọta ike nke fluorescence nke ọma na ọdịnaya FOB. Enwere ike ịchọpụta FOB dị na nlele site na onye nyocha fluorescence immunoassay.

Reagent na ihe ndị e nyere
Ngwa ngwugwu 25T:
Kaadị ule n'otu n'otu ejiri akpa 25T desiccant tinye ya
Ihe atụ diluents 25T
Ntinye ngwugwu 1
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge

NKWUKWU NA NKWUKWU
1.Jiri akpa dị ọcha nke a na-atụfu n'efu na-anakọta ụdị nsị ọhụrụ, wee nwalee ozugbo. Ọ bụrụ na enweghị ike ịnwale ozugbo, biko chekwaa na 2-8 Celsius maka ụbọchị 3 ma ọ bụ n'okpuru -15 Celsius maka ọnwa 6.
2.Wepụ osisi sampling, tinye n'ime ihe nlele ahụ, megharịa ihe ahụ ugboro 3, were akụkụ dị iche iche nke nsị ahụ n'oge ọ bụla, tinyezie osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, ihe dị ka 50mg feces sample, ma tinye n'ime a feces sample tube nwere sample dilution, na ịghasa tightly.
3.Jiri ihe atụfu pipette sampling were ihe nlele nsị si n'aka onye ọrịa afọ ọsịsa, tinyezie mmiri 3 (ihe dịka 100µL) na tube sampling fecal wee maa jijiji nke ọma.
ndetu:
1.Avoid ifriizi-thaw cycles.
2.Thaw samples ka ụlọ okpomọkụ tupu ojiji.
Usoro ASsay
Biko gụọ akwụkwọ ntuziaka ọrụ akụrụngwa na ntinye ngwugwu tupu ịnwale.
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
4.Wepụ kaadị ule site na akpa foil.
5.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6.Wepụ okpu si sample tube na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette.
7.Click bọtịnụ "ọkọlọtọ ule", mgbe nkeji iri na ise gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike ịgụ ihe si na ihuenyo ngosi nke ngwá ọrụ ahụ, ma dekọọ / bipụta nsonaazụ ule.
8.Rụrụ aka na ntuziaka nke Portable Immune Analyzer(WIZ-A101).
Ụkpụrụ echere
FOB <0.2μg/ml
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya na-anọchite anya ọnụ ọgụgụ ndị ọrịa ya.
Nsonaazụ ule na ntụgharị asụsụ
1.The FOB na sample bụ karịa na 0.2μg / mL, na kwesịrị ịchịkwa mgbanwe nke physiological ala. Nsonaazụ bụ n'ezie ihe na-adịghị mma ma ekwesịrị ịchọpụta ya na mgbaàmà ụlọ ọgwụ.
2.Nsonaazụ nke usoro a na-emetụta naanị n'ahịrị ntụaka nke e guzobere na usoro a, ọ dịghịkwa ihe jikọrọ ya na ụzọ ndị ọzọ.
3.Other ihe nwekwara ike ime ka njehie na nchọpụta nchọpụta, gụnyere teknuzu ihe, arụmọrụ njehie na ndị ọzọ sample ihe.

Nchekwa na Nkwụsi ike
1.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
2.Do not emepe mechiri emechi obere akpa ruo mgbe ị dị njikere ịrụ a ule, na otu-eji ule na-atụ aro na-eji n'okpuru chọrọ gburugburu ebe obibi (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. dị ka o kwere mee.
3.Sample diluent na-eji ozugbo emeghere ya.

Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ ​​​​na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
.AKWỤKWỌ reagents n'etiti ngwa dị iche iche nza No.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.

LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ime ihe atụ a. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka ule fecal. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.

ÀJỤMỤ ỌRỤ

REFERENCES
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.

 Igodo akara akara ejiri:

	Na Vitro Diagnostic Medical Device
	Onye nrụpụta
	Na-echekwa na 2-30 ℃
	Ụbọchị mmebi
	Ejighachila ya
	kpachara anya
	Kpọtụrụ ntuziaka maka ojiji

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