Nchọpụta Kit maka Estradiol(fluorescence immunoatographic)
Maka na vitro divostic na-eji naanị
Biko gụọ ngwugwu a tinye nke ọma tupu oge iji ma sorosie ntuziaka a. Enweghị ntụkwasị obi nke nsonaazụ arụpụtara enweghị ike ikwenye ma ọ bụrụ na enwere nrụpụta ọ bụla sitere na ntuziaka na ngwugwu a.

Eji eji
Nchọpụta Astradiol (Fluoorescence Immunographic nke Astanic Assadiographic maka nyocha nke Estradiol.it bụ ihe nlele nke Estradiol.it Ekwesịrị ikwenye site na usoro ndị ọzọ. Ezubere ule a maka iji ọrụ ọkachamara ahụike.

Nchikota
Estradiol (e2) bụ ihe kachasị mkpa na nke kachasị mkpa na nke estrogen.its dị arọ bụ 272,3 E2 a na-eji Plasenta, ebe ụmụ nwoke na-emepụta ihe site na Testicles.After E2 abanye na protein, 1% na-etinye ihe na-eme ka ọ bụrụ ihe na-acha uhie uhie (shbg), na ndị ọzọ na-ekekọta Albumin, metụta ya na imeju na sọlfọ ma ọ bụ n'ebe a na-eme ka mmamịrị, ma ọpụpụ site na mmamịrị.

Ụkpụrụ nke usoro
Thembrane nke ngwaọrụ nnwale a bụ ihe mkpuchi nke BSA na Estradiol na mpaghara nnwale na ewu oke oyibo oke bekee na mpaghara njikwa. A na-eji akara ngosi na-ekpo ọkụ na-achị achị A2 Antitiody na oke bekee na-aga n'ihu. Mgbe ịnwale nlele, E2 na Sample chịkọtara na fluorescence akara mgbochi e2 antimod, ma na-ejikọta ngwakọta mgbochi. N'okpuru ime amumumomatomatiographromatomatiographromatomatiograph, mgbagwoju anya na mpempe akwụkwọ na-agakọ, a ga-ejikọ akara ule ahụ, ịta ego nke Estradiol na-adịghị mma maka ihe mgbaàmà nke Estradio Ilebara Estradiol na nlele nwere ike ịchọpụta ihe mgbochi immuniassay.

Nta na ihe enyere

Ihe ngwungwu nke 25t:
.Test kaadị n'otu n'otu na-akpachapụrụ ya na 25t
.A ngwọta 25t
.B ngwọta 1
.Cage tinye 1

Ihe achọrọ mana enyeghị ya
Nzacha nchịkọta, oge

Nchịkọta nlele na nchekwa
1. Eserese na-enyocha nwere ike ịbụ n'ọbara, Hepatin anticoulantulant plasma ma ọ bụ Edta anticoulant plasma.
2.Ccording na usoro ọkọlọtọ na-anakọta nlele. Enwere ike idowe ọnya ma ọ bụ plasma nlegharị na 2-8 ℃ maka 7days na pricreroves dị n'okpuru -15 Celsius C ruo ọnwa isii.
.

Usoro nkwenye
Usoro nnwale nke ngwa ahụ na-ahụ akwụkwọ ntuziaka immunonalyher. Usoro nyocha ahụ dị ka ndị a
1.Lay wezuga onwe-ya na ihe nlere rue ulo okpomọkụ.
2.pen onye nyocha a na-etinyeghị ike (Wiz-A101), tinye paswọọdụ ndekọ akaụntụ dị ka usoro arụmọrụ nke ngwa ahụ, wee tinye ihe nchọpụta ahụ.
3.scan koodu ezé iji gosipụta ihe nnwale.
3.'tụpụ kaadị nnwale ahụ site na akpa foil.
Chee kaadi nnwale n'ime oghere kaadi, nyocha koodu QR, ma kpebie ihe nnwale.
5.AD 30μμL Serm ma ọ bụ ihe nlele plasma ka ọ bụrụ ihe ngwọta, ma gwakọta nke ọma.
6.add 20μL B Ngwọta na ngwakọta a dị n'elu, ma gwakọta nke ọma.
Hapụ ngwakọta maka20keotiti.
Tinye ngwakọta 80μL na grale nke ọma nke kaadị ahụ.
Pịa bọtịnụ "Elekere".
Rụtụ aka na ntụziaka nke onye nyocha nke onye nyocha nke onye nyocha a na-etinye (Wiz-A101).

Nsonaazụ nnwale na ịkọwa

.Ndị data dị n'elu bụ ntinye akwụkwọ nyocha nke ngwa ahịa a, a na-atụkwa aro na laabu na laabu nke ndi bi na mpaghara a di nkpa.
.Ndị estradiol dị elu karịa oke ntinye aka, yana mmeghachi omume physiological ma ọ bụ nzaghachi na-enweghị atụ, kwesịrị ijikọta nchọpụta ọgwụgwụ, ga-ejikọta ọgwụ mgbochi ọrịa.
.Ndị nsonaazụ a bụ naanị ntinye aka nke usoro a, nsonaazụ ya adịghị atụnyere usoro ndị ọzọ.
.

Nchekwa na nkwụsi ike
1.The Kit bụ ọnwa 18 na ndụ site na ụbọchị imepụta. Chekwaa Kits a na-ejighị na 2-30 Celsius C. Emela ihe. Ejila ụbọchị mmebi.
2.De emepeghị obere akpa ahụ ruo mgbe ị dịla njikere ịme ule, a na-atụ aro ka a na-ahụ maka otu ihe n'okpuru gburugburu chọrọ (okpomọkụ 40-90%) n'ime nkeji 60 ka enwere ike.
A na-eji dilute 3.sample dị ngwa mgbe a mepere ya.

Warningsdọ aka na ntị na akpachapụ anya
.The Kit ga-akara ma chekwaa ya pụọ ​​na mmiri.
.
.
.Da anaghị eji reserd resergent.
.Dlie abụghị ngwa ahịa n'etiti Kits na adịghị iche iche ..
.Na na-ejikwa kaadị ule na ngwa ọ bụla.
.Miche, oke nlele ma ọ bụ obere ihe nlele nwere ike ibute nsonaazụ.

LNnomi
.As na ihe ndị ọ bụla na-eme ihe na-eme ihe na-eme ihe na-arụ ọrụ, o nwere ike ime ihe mgbochi site na mgbochi mgbochi nke ụmụ mmadụ (HAMA) na ụdị. Specisens sitere n'aka ndị ọrịa natara nkwadebe nke nje mgbochi monoconosis ma ọ bụ ọgwụgwọ nwere ike ịnwe Hama. Ụdị ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ ụgha.
.Ndị nnwale ahụ bụ naanị maka ntụle ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nchọpụta ụlọ ọgwụ na ọgwụgwọ, ndị ọrịa na-ahụ maka nlekọta zuru oke yana mgbaàmà ya,
Akwukwo agha.
.HIGHIGHIRSASIRSA BITERRURU EGO NA-EGO NA MARA EGO. Ọ nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dị ka mmiri na mmamịrị na wdg.
Njirimara arụmọrụ

REnerise
1.Hassan JH, et Ntinye na ọgwụ ọjọọ nke sitere na immunody nke sitere na immunody [j].
2.levinson ss.The ọdịdị nke antibodic na intermomilic na intermomiasy miner (j] .j nke immunoasy, 1992: 108-114.

Igodo iji gosiputa ihe:

	Na vitronic Victostic ngwaọrụ
	Mmewe
	Chekwaa na 2-30 ℃
	Ụbọchị mmebi
	Etula ya
	Ikpachara anya
	Pụọ ntuziaka maka iji

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