Ngwa nyocha maka estradiol (nyocha immunochromatographic fluorescence)
Ngwa nyocha maka estradiol(immunochromatographic nyocha nke fluorescence)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.
Ezubere iji
Nchọpụta nchọpụta maka Estradiol (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka quantitative nchọpụta Estradiol (E2) na mmadụ serum ma ọ bụ plasma, nke a na-ejikarị enyocha ọkwa nke Estradiol.Ọ bụ ihe inyeaka nchoputa reagent.All nti sample sample. a ga-akwadorịrị site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.
Nchịkọta
Estradiol (E2) bụ hormone kachasị mkpa na nke kachasị arụ ọrụ na estrogen. Ya molecular arọ bụ 272.3 D. N'ozuzu, maka ndị inyom na-adịghị ime, E2 bụ tumadi secreted site n'ọbọ na granular cell na luteal cell n'oge follicular development.N'oge ime ime, E2 na-ezobe ya nke ọma site na placenta, ebe ụmụ nwoke na-emepụta tumadi site na testicles. Mgbe E2 batara n'ọbara, 1% ruo 3% adịghị ejikọta na protein, 40% na-ejikọta na homonụ mmekọahụ na-ejikọta globulin (SHBG) na ndị ọzọ. albumin, metabolize site na imeju banye sulfates mmiri na-agbaze ma ọ bụ gluconaldehyde esters, wee pụta na mmamịrị.E2 bụ ihe ngosipụta homonụ dị mkpa maka nyocha ọrụ ovarian.
Ụkpụrụ nke usoro
A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na conjugate nke BSA na Estradiol na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence antibody E2 na oke bekee IgG tupu oge eruo. Mgbe a na-anwale sample, E2 na sample jikọtara na fluorescence akara mgbochi E2 mgbochi, na-etolite dịghịzi ngwakọta. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent marker ga-ejikọta ya na Estradiol na akpụkpọ ahụ. The ịta nke Estradiol bụ ọjọọ mmekọrịta maka fluorescence mgbaàmà, na Enwere ike ịchọpụta mkpokọta estradiol na nlele site na nyocha nke fluorescence immunoassay.
Reagent na ihe ndị e nyere
Ngwa ngwugwu 25T:
.Kaadị ule n'otu n'otu n'otu n'otu foil nke e ji 25T desiccant tinye ya
.A ngwọta 25T
.B ngwọta 1
Ihe ngwungwu 1
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge
NKWUKWU NA NKWUKWU
1.The samples nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2.According ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.
All sample zere ifriizi-thaw cycles.
Usoro ASsay
Usoro ule nke ngwa ahụ hụ akwụkwọ ntuziaka immunoanalyzer. Usoro ule reagent bụ nke a
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
3.Wepụ kaadị ule site na akpa foil.
4.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
5.Tinye 30μL serum ma ọ bụ plasma sample na ngwọta, ma gwakọta nke ọma.
6.Tinye 20μL B ngwọta na ngwakọta n'elu, ma jikọta nke ọma.
Hapụ ngwakọta maka20nkeji.
Tinye ngwakọta 80μL ka ọ bụrụ nlele nke ọma nke kaadị ahụ.
Pịa bọtịnụ “ọkọlọtọ ule”, mgbe nkeji 10 gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike gụọ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, wee dekọọ / bipụta nsonaazụ ule.
Rụtụ aka na ntuziaka Portable Immune Analyzer(WIZ-A101).
Nsonaazụ ule na ntụgharị asụsụ
| Ogbo | Oke (pg/ml) | |
| Nwoke | 12.5-54.5 | |
| Nwanyị | usoro follicular | 28.5-185 |
| oge ovulatory | 81.5-408 | |
| Usoro luteal | 40.5-272 | |
| Menopause | 13.6-42.5 | |
.Ihe data dị n'elu bụ oge ntinye aka na-eguzobe maka nchọpụta nchọpụta nke kit a, a na-atụkwa aro na ụlọ nyocha ọ bụla kwesịrị ịmepụta oge ntinye aka maka mkpa ụlọ ọgwụ dị mkpa nke ndị bi na mpaghara a.
.Ntụkwasị obi nke Estradiol dị elu karịa ebe a na-ekwu okwu, na mgbanwe mgbanwe physiological ma ọ bụ nzaghachi nrụgide kwesịrị ịhapụ.
.Nsonaazụ nke usoro a na-emetụta naanị na nsonye ntụaka nke usoro a guzobere, na nsonaazụ adịghị atụnyere ya na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ime ka njehie na nsonaazụ nchọpụta, gụnyere ihe kpatara teknụzụ, njehie arụmọrụ na ihe nlele ndị ọzọ.
Nchekwa na Nkwụsi ike
1.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
2.Do not emepe mechiri emechi obere akpa ruo mgbe ị dị njikere ịrụ a ule, na otu-eji ule na-atụ aro na-eji n'okpuru chọrọ gburugburu ebe obibi (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. dị ka o kwere mee.
3.Sample diluent na-eji ozugbo emeghere ya.
Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
.AKWỤKWỌ reagents n'etiti ngwa na dị iche iche nza mba.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.
LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ime ihe atụ a. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nleba ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ahụike, ndị na-ahụ maka ụlọ ọgwụ kwesịrị ịdị na-atụgharị uche zuru oke yana ihe mgbaàmà ya,
.akụkọ ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ.
A na-eji reagent naanị maka nyocha ọbara na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.
ÀJỤMỤ ỌRỤ
| Linearity | 30 pg/mL ka 2000 pg/mL | Ngbanwe nke ikwu: -15% ruo +15%. |
| Ọnụọgụ njikọ linear:(r)≥0.9900 | ||
| Izi ezi | Ọnụego mgbake ga-adị n'ime 85% - 115%. | |
| Nkwagharị ugboro ugboro | CV≤15% | |
| Nkọwapụta(Ọnweghị nke ọ bụla n'ime ihe ndị a nwalere nnyonye anya etinyere na nyocha ahụ) | Ntinye aka | Ntinye uche na-etinye aka |
| T | 500ng/mL | |
| PROG | 500ng/mL | |
| Cor | 500ng/mL | |
| E3 | 100ng/mL | |
| 17β-E2 | 100ng/mL | |
REFERENCES
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.
Igodo akara akara ejiri:
 | Na Vitro Diagnostic Medical Device |
 | Onye nrụpụta |
 | Na-echekwa na 2-30 ℃ |
 | Ụbọchị mmebi |
 | Ejighachila ya |
 | kpachara anya |
 | Kpọtụrụ ntuziaka maka ojiji |
Ụgwọ nke ụlọ ọrụ Xiamen Wiz Biotech Co.,Ltd
okwu:3-4 Floor,NO.16 Building, Bio-medical Workshop,2030 Wengjiao West Road,Haicang District,361026,Xiamen,China
Tel:+86-592-6808278
Faksị: + 86-592-6808279
