Ngwa nyocha maka D-Dimer (nyocha immunochromatographic fluorescence)
Ngwa nyocha maka D-Dimer(immunochromatographic nyocha nke fluorescence)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.
Ezubere iji
Nchọpụta nchọpụta maka D-Dimer (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka nchọpụta quantitative nke D-Dimer (DD) na plasma mmadụ, a na-eji ya maka nchọpụta nke thrombosis venous, gbasaa coagulation intravascular, na nlekota nke thrombolytic. ọgwụgwọ .A ga-akwadorịrị ihe nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.
Nchịkọta
DD na-egosipụta ọrụ fibrinolytic. Ihe kpatara mmụba nke DD: 1. Hyperfibrinolysis nke abụọ, dị ka hypercoagulation, gbasaa coagulation intravascular, ọrịa akụrụ, nkwụsị nke akụkụ ahụ, ọgwụgwọ thrombolytic, wdg. ; 3.Myocardial infarction, cerebral infarction, pulmonary embolism, venous thrombosis, ịwa ahụ, akpụ, agbasa intravascular coagulation, ọrịa na anụ ahụ necrosis, wdg
Ụkpụrụ nke usoro
A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale na mgbochi DD mgbochi na mpaghara ule yana ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi DD mgbochi na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, DD antigen dị na sample na-ejikọta ya na fluorescence akpọrọ anti DD antibody, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara ya na mgbochi DD mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. A na-ejikọta ọkwa DD nke ọma na mgbama fluorescence, na ntinye nke DD na nlele nwere ike chọpụta site na fluorescence immunoassay assay.
Reagent na ihe ndị e nyere
Ngwa ngwugwu 25T:
Kaadị ule n'otu n'otu ejiri akpa 25T desiccant tinye ya
Ihe atụ diluents 25T
Ntinye ngwugwu 1
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge
NKWUKWU NA NKWUKWU
.Nlele a nwalere nwere ike ịbụ heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
.Dịka ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.
All sample zere ifriizi-thaw cycles.
Usoro ASsay
Biko gụọ akwụkwọ ntuziaka ọrụ akụrụngwa na ntinye ngwugwu tupu ịnwale.
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
4.Wepụ kaadị ule site na akpa foil.
5.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6.Add 40μL serum ma ọ bụ plasma sample ka sample diluent, na mix ọma ..
7.Add 80μL sample ngwọta ka ịlele nke ọma nke kaadị.
8.Click bọtịnụ "ọkọlọtọ ule", mgbe nkeji iri na ise gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike ịgụ ihe si na ihuenyo ngosi nke ngwá ọrụ ahụ, ma dekọọ / bipụta nsonaazụ ule.
9. Rụtụ aka na ntuziaka nke Portable Immune Analyzer(WIZ-A101).
Ụkpụrụ echere
DD <0.5mg/L
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya na-anọchite anya ọnụ ọgụgụ ndị ọrịa ya.
Nsonaazụ ule na ntụgharị asụsụ
.The n'elu data bụ n'ihi nke DD reagent ule, na ọ na-atụ aro na onye ọ bụla laabu kwesịrị guzobe a nso nke DD nchọpụta ụkpụrụ adabara ndị bi na mpaghara a. Nsonaazụ dị n'elu bụ naanị maka ntụnye aka.
.Nsonaazụ nke usoro a na-emetụta naanị na nsonye ntụaka nke e guzobere na usoro a, ọ dịghịkwa ihe jikọrọ ya na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ime ka njehie na nsonaazụ nchọpụta, gụnyere ihe kpatara teknụzụ, njehie arụmọrụ na ihe nlele ndị ọzọ.
Nchekwa na Nkwụsi ike
1.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
2.Do not emepe mechiri emechi obere akpa ruo mgbe ị dị njikere ịrụ a ule, na otu-eji ule na-atụ aro na-eji n'okpuru chọrọ gburugburu ebe obibi (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. dị ka o kwere mee.
3.Sample diluent na-eji ozugbo emeghere ya.
Ịdọ aka ná ntị na ịkpachara anya
1.The kit kwesịrị akara na-echebe megide mmiri.
2.A ga-eji usoro ndị ọzọ kwadoro ihe nlele niile dị mma.
3. A ga-ewere ụdịdị niile dị ka ndị nwere ike imetọ.
4.Ịghara iji reagent kubie ume.
5.DO adịghị agbanwe reagents n'etiti ngwa na dị iche iche nza No.
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7.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi ọdịiche.
LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ime ihe atụ a. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka nyocha ọbara na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.
ÀJỤMỤ ỌRỤ
| Linearity | 0.2mg/L ruo 10mg/l | Ngbanwe nke ikwu: -15% ruo +15%. |
| Ọnụọgụ njikọ linear:(r)≥0.9900 | ||
| Izi ezi | Ọnụego mgbake ga-adị n'ime 85% - 115%. | |
| Nkwagharị ugboro ugboro | CV≤15% | |
| Nkọwapụta(Ọnweghị nke ọ bụla n'ime ihe ndị a nwalere nnyonye anya etinyere na nyocha ahụ) | Ntinye aka | Ntinye uche na-etinye aka |
| FDP | 120mg/L | |
| VC | 2000mg/L | |
| Barbituric acid | 100mg/L | |
REFERENCES
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.
Igodo akara akara ejiri:
 | Na Vitro Diagnostic Medical Device |
 | Onye nrụpụta |
 | Na-echekwa na 2-30 ℃ |
 | Ụbọchị mmebi |
 | Ejighachila ya |
 | kpachara anya |
 | Kpọtụrụ ntuziaka maka ojiji |
Ụgwọ nke ụlọ ọrụ Xiamen Wiz Biotech Co.,Ltd
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