Ngwa nyocha maka Calprotectin (Fluorescence Immunochromatographic assay)
Ngwa nyocha makaCalprotectin(Fluorescence Immunochromatographic assay)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.
Ezubere iji
Ngwa nyocha makaCalprotectin(Cal) kwesịrị ekwesị maka ikpebi ọnụọgụgụ nke nsị mmadụ Cal site na fluorescence immunochromatographic assay, nke nwere uru nchọpụta ngwa ngwa dị mkpa maka ọrịa eriri afọ na-egbuke egbuke. A ghaghị ịkwado ihe nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.
Nchịkọta
Cal bụ heterodimer, nke mejupụtara MRP 8 na MRP 14[1]. Ọ dị na cytoplasm neutrophils ma gosipụta ya na membranes cell mononuclear. Cal bụ protein na-agba agba, ọ nwere usoro kwụsiri ike nke ọma ihe dị ka otu izu n'ime afọ mmadụ, a na-ekpebisi ike ịbụ akara ọrịa afọ ọzịza.[2-3]. Ngwa ahụ bụ ule dị mfe, nke na-ahụ anya nke na-achọpụta Cal na nsị mmadụ, ọ nwere nghọta dị elu na nkọwa siri ike. Nnwale ahụ dabere na nkọwapụta dị elu nke ụkpụrụ mmeghachi omume Sanwichi mgbochi abụọ yana fluorescence immunochromatographic assay analysis technics, nke nwere ike inye nsonaazụ n'ime nkeji iri na ise.
Ụkpụrụ nke usoro
Iberibe ahụ nwere mgbochi mkpuchi Cal na mpaghara nnwale, nke etinyere na chromatography akpụkpọ ahụ tupu oge eruo. A na-ekpuchi pad lable site na fluorescence akpọrọ anti-Cal antibody tupu oge eruo. Mgbe a na-anwale nlele dị mma, Cal dị na nlele nwere ike ịgwakọta ya na fluorescence akpọrọ anti-Cal antibody, wee mepụta ngwakọta mgbochi. Ka a na-ekwe ka ngwakọta ahụ kwaga n'akụkụ ebe nnwale ahụ, ihe mgbochi Cal conjugate na-ejide ihe mgbochi Cal conjugate na akpụkpọ ahụ wee mepụta mgbagwoju anya. Ike fluorescence na-ejikọta nke ọma na ọdịnaya Cal. Enwere ike ịchọpụta Cal dị na nlele site na onye nyocha fluorescence immunoassay.
Reagent na ihe ndị e nyere
Ngwa ngwugwu 25T:
Kaadị ule n'otu n'otu ejiri akpa 25T desiccant tinye ya
Ihe atụ diluents 25T
Ntinye ngwugwu 1
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge
NKWUKWU NA NKWUKWU
1.Jiri akpa dị ọcha nke a na-atụfu n'efu na-anakọta ụdị nsị ọhụrụ, wee nwalee ozugbo. Ọ bụrụ na enweghị ike ịnwale ozugbo, biko chekwaa na 2-8 Celsius maka ụbọchị 3 ma ọ bụ n'okpuru -15 Celsius maka ọnwa 6.
2.Wepụ osisi sampling, tinye n'ime ihe nlele ahụ, megharịa ihe ahụ ugboro 3, were akụkụ dị iche iche nke nsị ahụ n'oge ọ bụla, tinyezie osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, ihe dị ka 50mg feces sample, ma tinye n'ime a feces sample tube nwere sample dilution, na ịghasa tightly.
3.Jiri ihe atụfu pipette sampling were ihe nlele nsị si n'aka onye ọrịa afọ ọsịsa, tinyezie mmiri 3 (ihe dịka 100µL) na tube sampling fecal wee maa jijiji nke ọma.
ndetu:
1.Avoid ifriizi-thaw cycles.
2.Thaw samples ka ụlọ okpomọkụ tupu ojiji.
Usoro ASsay
Biko gụọ akwụkwọ ntuziaka ọrụ akụrụngwa na ntinye ngwugwu tupu ịnwale.
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
4.Wepụ kaadị ule site na akpa foil.
5.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6.Wepụ okpu si sample tube na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette.
7.Click bọtịnụ "ọkọlọtọ ule", mgbe nkeji iri na ise gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike ịgụ ihe si na ihuenyo ngosi nke ngwá ọrụ ahụ, ma dekọọ / bipụta nsonaazụ ule.
8.Rụrụ aka na ntuziaka nke Portable Immune Analyzer(WIZ-A101).
Ụkpụrụ echere
Cal <60μg/g
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya na-anọchite anya ọnụ ọgụgụ ndị ọrịa ya.
Nsonaazụ ule na ntụgharị asụsụ
1.The Cal na sample bụ karịa na 60μg / g, na kwesịrị ịchịkwa mgbanwe nke physiological ala. Nsonaazụ bụ n'ezie ihe na-adịghị mma ma ekwesịrị ịchọpụta ya na mgbaàmà ụlọ ọgwụ.
2.Nsonaazụ nke usoro a na-emetụta naanị n'ahịrị ntụaka nke e guzobere na usoro a, ọ dịghịkwa ihe jikọrọ ya na ụzọ ndị ọzọ.
3.Other ihe nwekwara ike ime ka njehie na nchọpụta nchọpụta, gụnyere teknuzu ihe, arụmọrụ njehie na ndị ọzọ sample ihe.
Nchekwa na Nkwụsi ike
1.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
2.Do not emepe mechiri emechi obere akpa ruo mgbe ị dị njikere ịrụ a ule, na otu-eji ule na-atụ aro na-eji n'okpuru chọrọ gburugburu ebe obibi (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. dị ka o kwere mee.
3.Sample diluent na-eji ozugbo emeghere ya.
Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
.AKWỤKWỌ reagents n'etiti ngwa dị iche iche nza No.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.
MAKA
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké eme ihe, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ụdị. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka ule fecal. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.
ÀJỤMỤ ỌRỤ
Linearity | 10μg/g ruo 2400μg/g | Ngbanwe nke ikwu: -15% ruo +15%. |
Ọnụọgụ njikọ linear:(r)≥0.9900 | ||
Izi ezi | Ọnụego mgbake ga-adị n'ime 85% - 115%. | |
Nkwagharị ugboro ugboro | CV≤15% | |
Nkọwapụta (Ọ nweghị ihe ọ bụla n'ime ihe ndị nnyonye anya nwalere etinyere na nyocha ahụ) | Ntinye aka | Ntinye uche na-etinye aka |
Hemoglobin | 200μg / ml | |
transferrin | 100μg/ml | |
Ịnyịnya radish peroxidase | 2000μg/ml |
Ntụaka
1.Li, G.&Y.L.Li. Mmekọrịta dị n'etiti calcium na ọrịa ụlọ ọgwụ [J] Journal of Practical Medicine, 2007,23 (15)
2.Han,W.,Xu,JM,et al.The Clinical ọmụmụ nke calamine na lactoferrin na ọrịa na ulcerative colitis[J].Chinese Journal of Gastroenterology,2010,15(4)
3.Wang,ZH,Guo,HB,et al.Ọmụmụ ihe gbasara mmekọrịta dị n'etiti fecal calamine na ọrịa na-egbu egbu [J]. Teknụzụ sayensị na injinia, 2010-03,10 (8)
Igodo akara akara ejiri:
Na Vitro Diagnostic Medical Device | |
Onye nrụpụta | |
Na-echekwa na 2-30 ℃ | |
Ụbọchị mmebi | |
Ejighachila ya | |
kpachara anya | |
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