Ngwa nyocha makaprotein C-reactive hypersensitive

(immunochromatographic nyocha nke fluorescence)

N'ihi na in vitro diagnostic ojiji naanị

Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji

Kit diagnostic maka hypersensitive C-reactive protein (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka nyocha quantitative nke C-reactive protein (CRP) na ọbara mmadụ /plasma/ dum ọbara. Ọ bụ ihe na-egosi mbufụt nke na-abụghị nke akọwapụtara. A ga-akwadorịrị nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta

Protein C-reactive bụ nnukwu protein nke na-emepe emepe site na mkpali lymphokine nke imeju na sel epithelial. Ọ dị na ọbara mmadụ, mmiri ụbụrụ cerebrospinal, pleural na abdominal fluid, wdg, ma bụrụ akụkụ nke usoro nchebe na-abụghị nke a kapịrị ọnụ. 6-8h mgbe ihe omume nke nje nje, CRP malitere ịba ụba, 24-48h ruru elu, na ọnụ ọgụgụ kasị elu nwere ike iru ọtụtụ narị ugboro nkịtị. Mgbe e kpochapụrụ ọrịa ahụ, CRP dara nke ọma wee laghachi na nkịtị n'ime otu izu. Otú ọ dị, CRP adịghị amụba nke ọma n'ihe banyere ọrịa nje, nke na-enye ihe ndabere maka njirimara nke ụdị ọrịa nrịanrịa n'oge, ma bụrụ ngwá ọrụ iji chọpụta ọrịa nje ma ọ bụ nje.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ nnwale ahụ na mgbochi CRP na mpaghara ule yana mgbochi ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi CRP antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, antigen CRP dị na sample na-ejikọta ya na fluorescence akpọrọ mgbochi CRP antibody, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara na mgbochi CRP mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. A na-ejikọta ọkwa CRP nke ọma na mgbaàmà fluorescence, na ntinye nke CRP na sample nwere ike chọpụta site na fluorescence immunoassay assay.

Reagent na ihe ndị e nyere

Ngwa ngwugwu 25T:

Kaadị ule n'otu n'otu ejiri akpa 25T desiccant tinye ya

Ihe atụ diluents 25T

Ntinye ngwugwu 1

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ

Akpa mkpokọta ihe nlele, ngụ oge

NKWUKWU NA NKWUKWU

1. Ihe nlele a nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2. Dị ka ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6. Enwere ike idowe ihe nlele ọbara niile na friji na 2-8 ℃ maka ụbọchị 3
3. Nlereanya niile na-ezere okirikiri ịsa oyi.