Nchọpụta Kit maka alfa-fetproptein (fluorescence immunochiographic)
Nchọpụta Kit maka alfa-fetproptuin(fluorescence immunoatographic)
Maka na vitro divostic na-eji naanị
Biko gụọ ngwugwu a tinye nke ọma tupu oge iji ma sorosie ntuziaka a. Enweghị ntụkwasị obi nke nsonaazụ arụpụtara enweghị ike ikwenye ma ọ bụrụ na enwere nrụpụta ọ bụla sitere na ntuziaka na ngwugwu a.
Eji eji
Nchọpụta Kit maka Alfa-FetPoprotein (Fluoooocenence Immunography Asinhoadographic maka nchọpụta nke Alfa-Fetoprotein (AFP) nke AUXAION, mmetụta ịgwọ ọrịa na precosinomilar Carcinoma. Ekwesịrị ijite usoro ihe atụ ndị ọzọ dị mma. Ezubere ule a maka iji ọrụ ọkachamara ahụike.
Nchikota
Afa-fetproptuin (afp) bụ otu n'ime ihe ndị na-eme ihe na-ejikarị 70,000 na shuga na-ejikarị ya. Izu isii 90 ruo 500 ng / ml n'etiti; ọdịnaya Ọdịnaya AFP dị n'agbata 2 na 8 ng / ml, mana ọtụtụ ọrịa, na-emetụta uru AFP.
Ụkpụrụ nke usoro
Thembrane nke ngwaọrụ nnwale a na-ejikọ na mpaghara nnwale ma wuo mgbochi oke bekee IGG Angumpy na mpaghara njikwa. A na-eji akwa igwe na-ebugharị site na fluoorcence na-akpọ Antirod Antiyody na oke bekee IGG tupu oge eruo. Mgbe ị na-anwale ezigbo nlele, afp antigen na nlele na fluored na-ejikọ mgbochi Antirod, yana ngwakọta mgbochi ọrịa. N'okpuru ime ihe nke immunochromatomatomatigraphromatomatiographromatomatiograph, usoro mgbagwoju anya na ntuziaka na-atọ ụtọ, ya na acpasing na AFPPED dị iche iche. Na nlele ka enwere ike ịchọpụta ihe mgbochi immuniassay.
Nta na ihe enyere
Ihe ngwungwu nke 25t:
.Test kaadị n'otu n'otu na-akpachapụrụ ya na 25t
.Sample nwere 25t
.Cage tinye 1
Ihe achọrọ mana enyeghị ya
Nzacha nchịkọta, oge
Nchịkọta nlele na nchekwa
1. Eserese na-enyocha nwere ike ịbụ n'ọbara, Hepatin anticoulantulant plasma ma ọ bụ Edta anticoulant plasma.
2.Ccording na usoro ọkọlọtọ na-anakọta nlele. Enwere ike idowe ọnya ma ọ bụ plasma nlegharị na 2-8 ℃ maka 7days na pricreroves dị n'okpuru -15 Celsius C ruo ọnwa isii.
3. Samall Sale na-ezere crycles na-akwụ ụgwọ.
Usoro nkwenye
Biko gụọ akwụkwọ ntuziaka na ngwugwu ngwa ọrụ tupu nnwale.
1.Lay wezuga onwe-ya na ihe nlere rue ulo okpomọkụ.
2.pen onye nyocha a na-etinyeghị ike (Wiz-A101), tinye paswọọdụ ndekọ akaụntụ dị ka usoro arụmọrụ nke ngwa ahụ, wee tinye ihe nchọpụta ahụ.
3.scan koodu ezé iji gosipụta ihe nnwale.
4.Nata kaadị nnwale ahụ site na akpa foil.
Kechie kaadị nnwale ahụ n'ime oghere kaadị ahụ, nyochaa koodu QR, ma kpebie ihe nnwale ahụ.
6.Add 20μL Serm ma ọ bụ ihe nlele plasma iji mee ihe atụ, ma gwakọta nke ọma ..
7.00μL nlele nlele maka eserese nke kaadị a.
8. Pịa bọtịnụ "Elekere ọkọlọtọ", mgbe minit 15 gasịrị, ngo ahụ chọpụta kaadị nnwale ahụ, ọ nwere ike gụọ nsonaazụ ya site na ihuenyo ngosipụta, na ndekọ / dekọọ ya / Binye nsonaazụ ule.
9.Refer na ntuziaka nke onye nyocha nke onye nyocha nke onye nyocha nke a na-etinye (Wiz-A101).
Ụkpụrụ a tụrụ anya ya
AFP: <10ng / ml
A na-atụ aro ka ụlọ nyocha ọ bụla guzobere ụdị ndị ọ na-anọchi anya ya.
Nsonaazụ nnwale na ịkọwa
.Ndị data dị elu bụ nsonaazụ AFP resergent, a na-atụ aro na laabu na laabu nke a na-achọpụta ihe dị mma maka ndị bi na mpaghara a. Nsonaazụ a dị n'elu bụ maka naanị.
.Na usoro a bụ naanị ihe arụ ọrụ dị na akwụkwọ ntụaka guzobere n'ụzọ a, ọ nweghịkwa ọnụ ọgụgụ dị iche iche na ụzọ ndị ọzọ.
.
Nchekwa na nkwụsi ike
1.The Kit bụ ọnwa 18 na ndụ site na ụbọchị imepụta. Chekwaa Kits a na-ejighị na 2-30 Celsius C. Emela ihe. Ejila ụbọchị mmebi.
2.De emepeghị obere akpa ahụ ruo mgbe ị dịla njikere ịme ule, a na-atụ aro ka a na-ahụ maka otu ihe n'okpuru gburugburu chọrọ (okpomọkụ 40-90%) n'ime nkeji 60 ka enwere ike.
A na-eji dilute 3.sample dị ngwa mgbe a mepere ya.
Warningsdọ aka na ntị na akpachapụ anya
.The Kit ga-akara ma chekwaa ya pụọ na mmiri.
.
.
.Da anaghị eji reserd resergent.
. Ọ bụghị ngwa ahịa n'etiti Kits na nza adịghị adị ..
.Na na-ejikwa kaadị ule na ngwa ọ bụla.
.Miche, oke nlele ma ọ bụ obere ihe nlele nwere ike ibute nsonaazụ.
LNnomi
.As na ihe ndị ọ bụla na-eme ihe na-eme ihe na-eme ihe na-arụ ọrụ, o nwere ike ime ihe mgbochi site na mgbochi mgbochi nke ụmụ mmadụ (HAMA) na ụdị. Specisens sitere n'aka ndị ọrịa natara nkwadebe nke nje mgbochi monoconosis ma ọ bụ ọgwụgwọ nwere ike ịnwe Hama. Ụdị ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ ụgha.
.Ndị nnwale ahụ bụ naanị maka ntụle ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe kpatara ya, nyocha ahụike, nzaghachi, mmeghachi omume, EpideMology na ozi ndị ọzọ .
.HIGHIGHIRSASIRSA BITERRURU EGO NA-EGO NA MARA EGO. Ọ nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dị ka mmiri na mmamịrị na wdg.
Njirimara arụmọrụ
| Mmegide | 1ng / ml ruo 1000ng / ml | Ntinye uche: -15% ka 15%. |
| CoghteFerition na-akwụ ụgwọ: (R) ≥0.9900 | ||
| Kpomkwem | Ọnụego mgbake ahụ ga-abụ n'ime 85% - 115%. | |
| Ikwughari | Cv≤15% | |
| Nkọwapụta (ọ nweghị nke na-eme ihe na-agbanwe agbanwe | Kenngba ume | Ntinye uche |
| Acetaminophen | 1500μG / ml | |
| Acetylssaylic acid | 10mg / ml | |
| Eri | 500μ / ml | |
| Emoglobin | 200μG / ml | |
| mbufe | 100μG / ml | |
| Horse Radish peroxidase | 2000μg / ml | |
| LH | 200miu / ml | |
| Fesh | 200miu / ml | |
| Hcg | 20000miu / ml | |
| Tosh | 200μμUU / ml | |
| Bsa | 5mg / ml | |
| Vinastine | 500μ / ml | |
| Cisplatin | 1000μg / ml | |
| Azathipline | 30mg / l | |
| BloMycin | 100μU / ml | |
REnerise
1.Hassan JH, et Ntinye na ọgwụ ọjọọ nke sitere na immunody nke sitere na immunody [j].
2.levinson ss.The ọdịdị nke antibodic na intermomilic na intermomiasy miner (j] .j nke immunoasy, 1992: 108-114.
Igodo iji gosiputa ihe:
 | Na vitronic Victostic ngwaọrụ |
 | Mmewe |
 | Chekwaa na 2-30 ℃ |
 | Ụbọchị mmebi |
 | Etula ya |
 | Ikpachara anya |
 | Pụọ ntuziaka maka iji |
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