Ngwa nyocha maka 25-hydroxy Vitamin D (nyocha nyocha nke immunochromatographic fluorescence)
Ngwa nyocha maka 25-hydroxy Vitamin D(immunochromatographic nyocha nke fluorescence)
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.
Ezubere iji
Nchọpụta nchọpụta maka 25-hydroxy Vitamin D (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka nchọpụta quantitative nke 25-hydroxy Vitamin D (25- (OH) VD) na mmadụ serum ma ọ bụ plasma, nke a na-ejikarị iji nyochaa ọkwa. nke vitamin D.Ọ bụ ihe inyeaka nchoputa reagent.All nti sample ga-akwado ndị ọzọ methodologies. Ezubere ule a maka naanị ndị ọkachamara ahụike.
Nchịkọta
Vitamin D bụ vitamin ma bụrụkwa homonụ steroid, nke gụnyere VD2 na VD3, nke usoro ha yiri nnọọ. Vitamin D3 na D2 na-atụgharị gaa na 25 hydroxyl vitamin D (gụnyere 25-dihydroxyl vitamin D3 na D2). 25- (OH) VD n'ime ahụ mmadụ, nkwụsi ike struction, elu itinye uche. 25- (OH) VD na-egosipụta ọnụọgụ vitamin D zuru oke, na ikike ngbanwe nke vitamin D, ya mere 25- (OH) VD na-ewere dị ka ihe ngosi kachasị mma maka ịlele ọkwa vitamin D. Ihe nchọpụta nchọpụta dabeere na ya. Immunochromatography ma nwee ike inye nsonaazụ n'ime nkeji iri na ise.
Ụkpụrụ nke usoro
A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na conjugate nke BSA na 25- (OH) VD na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence anti 25-(OH) VD mgbochi na oke bekee IgG tupu oge eruo. Mgbe a na-anwale sample, 25- (OH) VD na sample jikọtara na fluorescence akara mgbochi 25- (OH) VD mgbochi, na-etolite dịghịzi ngwakọta. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent akara ga-ejikọta na 25- (OH) VD na akpụkpọ ahụ. The ịta nke 25- (OH). VD bụ ihe na-adịghị mma maka mgbaàmà fluorescence, yana ntinye nke 25- (OH) VD na nlele nwere ike chọpụta site na fluorescence immunoassay assay.
Reagent na ihe ndị e nyere
Ngwa ngwugwu 25T:
.Kaadị ule n'otu n'otu n'otu n'otu foil nke e ji 25T desiccant tinye ya
.A ngwọta 25T
.B ngwọta 1
Ihe ngwungwu 1
AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ
Akpa mkpokọta ihe nlele, ngụ oge
NKWUKWU NA NKWUKWU
1.The samples nwalere nwere ike ịbụ serum, heparin anticoagulant plasma ma ọ bụ EDTA anticoagulant plasma.
2.According ọkọlọtọ usoro na-anakọta sample. Enwere ike idowe ihe nlele ọbara ma ọ bụ plasma refrigerated na 2-8 ℃ maka ụbọchị 7 na cryopreservation n'okpuru -15 Celsius maka ọnwa 6.
3.All sample zere ifriizi-thaw cycles.
Usoro ASsay
Usoro ule nke ngwa ahụ hụ akwụkwọ ntuziaka immunoanalyzer. Usoro ule reagent bụ nke a
1.Dịna n'akụkụ niile reagents na sample na ụlọ okpomọkụ.
2.Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nlele ahụ.
4.Wepụ kaadị ule site na akpa foil.
5.Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6.Add 30μL serum ma ọ bụ plasma sample na A ngwọta, na mix ọma.
7.Tinye 50μL B ngwọta na ngwakọta dị n'elu, ma jikọta nke ọma.
8.Hapụ ngwakọta maka nkeji iri na ise.
9.Tinye ngwakọta 80μL ka ọ bụrụ nlele nke ọma nke kaadị ahụ.
10.Pịa bọtịnụ "ọkọlọtọ ule", mgbe nkeji 10 gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike ịgụ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, wee dekọọ / bipụta nsonaazụ ule.
11. Rụtụ aka na ntuziaka nke Portable Immune Analyzer (WIZ-A101).
Ụkpụrụ echere
25- (OH) VD nkịtị nso: 30-100ng / ml
A na-atụ aro ka ụlọ nyocha ọ bụla guzobe oke nke ya na-anọchite anya ọnụ ọgụgụ ndị ọrịa ya.
Nsonaazụ ule na ntụgharị asụsụ
.Ihe data dị n'elu bụ oge ntinye aka na-eguzobe maka nchọpụta nchọpụta nke kit a, a na-atụkwa aro na ụlọ nyocha ọ bụla kwesịrị ịmepụta oge ntinye aka maka mkpa ụlọ ọgwụ dị mkpa nke ndị bi na mpaghara a.
.Nkwadebe nke 25- (OH) VD dị elu karịa ebe a na-ekwu okwu, a ga-ewepụkwa mgbanwe physiological ma ọ bụ nzaghachi nrụgide. N'ezie ihe na-adịghị mma, kwesịrị ijikọta nchọpụta mgbaàmà ahụike.
.Nsonaazụ nke usoro a na-emetụta naanị na nsonye ntụaka nke usoro a guzobere, na nsonaazụ adịghị atụnyere ya na ụzọ ndị ọzọ.
.Ihe ndị ọzọ nwekwara ike ime ka njehie na nsonaazụ nchọpụta, gụnyere ihe kpatara teknụzụ, njehie arụmọrụ na ihe nlele ndị ọzọ.
Nchekwa na Nkwụsi ike
.The kit bụ 18 ọnwa shelf-ndụ site na ụbọchị mmepụta. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emela friji. Ejila ụbọchị ngafe gafere.
.Emepela obere akpa ahụ mechiri emechi ruo mgbe ị dị njikere ịme ule, a na-atụ aro ka a na-eji ule otu-eji n'okpuru ebe achọrọ (okpomọkụ 2-35 ℃, iru mmiri 40-90%) n'ime 60 mins ngwa ngwa. kwere omume.
.Sample diluent na-eji ozugbo emeghere.
Ịdọ aka ná ntị na ịkpachara anya
.A ga-emechi ngwa ahụ ma chebe ya pụọ na mmiri.
.A ga-eji usoro ndị ọzọ kwado ihe nlere niile dị mma.
.A ga-ewere ụdịdị niile dị ka ihe nwere ike imetọ.
.Ejila reagenti mebiela.
.AKWỤKWỌ reagents n'etiti ngwa na dị iche iche nza mba.
.Ejikwala kaadị ule na ngwa ọ bụla enwere ike ijikwa ọzọ.
.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi deviations.
LNṅomi
Dị ka ọ dị na nyocha ọ bụla na-eji ọgwụ mgbochi òké, ohere dị maka nnyonye anya site na mgbochi mgbochi òké mmadụ (HAMA) n'ime ihe atụ a. Ihe atụ sitere na ndị ọrịa natara ọgwụ mgbochi monoclonal maka nchoputa ma ọ bụ ọgwụgwọ nwere ike ịnwe HAMA. Ụdị ụdị ahụ nwere ike ịkpata nsonaazụ na-adịghị mma ma ọ bụ ụgha ụgha.
.Nnwale ule a bụ naanị maka ntụaka ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe ndabere maka nyocha na ọgwụgwọ ụlọ ọgwụ, ndị na-ahụ maka ụlọ ọgwụ na-ahụ maka ndị ọrịa kwesịrị ịdị na-atụgharị uche zuru oke na-ejikọta ya na mgbaàmà ya, akụkọ ahụike ahụike, nyocha ụlọ nyocha ndị ọzọ, nzaghachi ọgwụgwọ, ọrịa na-efe efe na ozi ndị ọzọ. .
A na-eji reagent naanị maka nyocha ọbara na plasma. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.
ÀJỤMỤ ỌRỤ
Linearity | 5ng/ml ka 120ng/mL | Ngbanwe nke ikwu: -15% ruo +15%. |
Ọnụọgụ njikọ linear:(r)≥0.9900 | ||
Izi ezi | Ọnụego mgbake ga-adị n'ime 85% - 115%. | |
Nkwagharị ugboro ugboro | CV≤15% | |
Nkọwapụta (Ọnweghị nke ọ bụla n'ime ihe ndị a nwalere nnyonye anya etinyere na nyocha ahụ) | Ntinye aka | Ntinye uche na-etinye aka |
Hemoglobin | 200μg / ml | |
transferrin | 100μg/ml | |
Ịnyịnya radish peroxidase | 2000μg/ml | |
Vitamin D3 | 50mg/mL | |
Vitamin D | 50mg/mL |
REFERENCES
1.Hansen JH, et al.HAMA Ntinye aka na Murine Monoclonal Antibody-based Immunoassays[J].J nke Clin Immunoassay,1993,16:294-299.
2.Levinson SS. Ọdịdị nke Antibodies Heterophilic na Ọrụ dị na Ntinye aka Immunoassay [J].J nke Clin Immunoassay,1992,15:108-114.
Igodo akara akara ejiri:
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Ejighachila ya | |
kpachara anya | |
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