Nchọpụta Kit maka vitamin 25-Hydroxy D(fluorescence immunoatographic)
Maka na vitro divostic na-eji naanị
Biko gụọ ngwugwu a tinye nke ọma tupu oge iji ma sorosie ntuziaka a. Enweghị ntụkwasị obi nke nsonaazụ arụpụtara enweghị ike ikwenye ma ọ bụrụ na enwere nrụpụta ọ bụla sitere na ntuziaka na ngwugwu a.

Eji eji
Nchọpụta Nchọpụta 25-Hydrooxy Vitamin D (Flu fluosoro immunochigraphic nke na-eme ihe mgbochi nke 25-hydroxy vitamin ma ọ bụ plasma, nke a na-ejikarị nyochaa ọkwa Nke vitamin D.it bụ nchọpụta inyeaka aurent.All dị mma maka usoro ndị ọzọ mebere. Ezubere ule a maka iji ọrụ ọkachamara ahụike.

Nchikota
Vitamin D bụ vitamin ma bụkwa homonụ sterono, nke gụnyere VD2 na VD3, nke a na-akụda ya yiri nke a. A na-agbanwe vitamin D3 na D2 vitamin 25 Hydroxyl vitamin D (gụnyere vitamin 25 na D2). 25- (Oh) VD n'ime ahụ mmadụ, azọda siri ike, dị elu itinye uche. 25- (Oh) VD na-egosipụta mkpokọta vitamin D, yana ike ntụgharị nke vitamin D, yabụ 25- (oh) na-egosi ọkwa nke vitamin D.TE dabere na Immunocromatography ma nwee ike ịnye nsonaazụ n'ime nkeji iri na ise.

Ụkpụrụ nke usoro
Thembrane nke ngwaọrụ nnwale ahụ bụ ihe mkpuchi nke BSA na 25- (oh) VD na mpaghara nnwale na ewu oke oyibo IGG Antody na mpaghara njikwa. A na-eji akara ngosi na-enweghị nsị na-achị achị na-alụ ọgụ na-emegide 25- (oh) VD Antody na oke bekee Igwe tupu oge eruo. Mgbe ịnwale nlele, 25- (oh) vd na sample chịkọtara na fluorescence akara mgbochi, (oh) mọstad, ma ngwakọta mgbochi ọrịa. N'okpuru ime amumumomigraphromatomatiographromimatomatiographromimatomatiograph, usoro mgbagwoju anya na ntuziaka na-atọ ụtọ, mgbe ọ ga-ejikọ akara ule ahụ, na-akwụghị ụgwọ VD bụ njikọ na-adịghị mma maka mgbaàmà nke flioorcence, na ịta nke 25- (oh) na-achọpụta VD nwere enwere ike ịchọpụta na a nwere ike ịchọpụta na a ga-achọpụta ọgwụ mgbochi mmiri.

Nta na ihe enyere

Ihe ngwungwu nke 25t:
.Test kaadị n'otu n'otu na-akpachapụrụ ya na 25t
.A ngwọta 25t
.B ngwọta 1
.Cage tinye 1

Ihe achọrọ mana enyeghị ya
Nzacha nchịkọta, oge

Nchịkọta nlele na nchekwa
1. Eserese na-enyocha nwere ike ịbụ n'ọbara, Hepatin anticoulantulant plasma ma ọ bụ Edta anticoulant plasma.
2.Ccording na usoro ọkọlọtọ na-anakọta nlele. Enwere ike idowe ọnya ma ọ bụ plasma nlegharị na 2-8 ℃ maka 7days na pricreroves dị n'okpuru -15 Celsius C ruo ọnwa isii.
3. Samall Sale na-ezere crycles na-akwụ ụgwọ.

Usoro nkwenye
Usoro nnwale nke ngwa ahụ na-ahụ akwụkwọ ntuziaka immunonalyher. Usoro nyocha ahụ dị ka ndị a
1.Lay wezuga onwe-ya na ihe nlere rue ulo okpomọkụ.
2.pen onye nyocha a na-etinyeghị ike (Wiz-A101), tinye paswọọdụ ndekọ akaụntụ dị ka usoro arụmọrụ nke ngwa ahụ, wee tinye ihe nchọpụta ahụ.
3.scan koodu ezé iji gosipụta ihe nnwale.
4.Nata kaadị nnwale ahụ site na akpa foil.
Kechie kaadị nnwale ahụ n'ime oghere kaadị ahụ, nyochaa koodu QR, ma kpebie ihe nnwale ahụ.
6.Add 30μL Serm ma ọ bụ ihe nlele plasma ka ọ bụrụ ihe ngwọta, ma gwakọta nke ọma.
7.DD 50μL B Ngwọta na ngwakọta a dị n'elu, ma gwakọta nke ọma.
8.Hapụ ngwakọta ahụ ruo minit 15.
9.0cad 80μL ngwakọta na grale nke ọma nke kaadị a.
3. Pịa bọtịnụ "Elekere ọkọlọtọ", mgbe minit 10 gachara, ngwa ahụ achọpụtala kaadị nnwale ahụ, ọ nwere ike gụọ nsonaazụ ya site na ihuenyo ngosipụta ya, ma detuo / Binye nsonaazụ ule.
11.aref na ntuziaka nke onye nyocha nke onye nyocha nke onye nyocha nke onye nyocha nke a na - ebuso ya (Wiz-A101).

Ụkpụrụ a tụrụ anya ya
25- (oh) VD dị mma: 30-100ng / ml
A na-atụ aro ka ụlọ nyocha ọ bụla guzobere ụdị ndị ọ na-anọchi anya ya.

Nsonaazụ nnwale na ịkọwa
.Ndị data dị n'elu bụ ntinye akwụkwọ nyocha nke ngwa ahịa a, a na-atụkwa aro na laabu na laabu nke ndi bi na mpaghara a di nkpa.
.Ndị na-atọ ụtọ nke 25- (oh) VD dị elu karịa oke ntinye aka, yana mmeghachi omume physiological ma ọ bụ mmeghachi omume na-enweghị atụ, kwesịrị ijikọta nyocha nke ọrịa.
.Ndị nsonaazụ a bụ naanị ntinye aka nke usoro a, nsonaazụ ya adịghị atụnyere usoro ndị ọzọ.
.

Nchekwa na nkwụsi ike
.The Kit bụ ọnwa 18 ọnwa site na ụbọchị imepụta. Chekwaa Kits a na-ejighị na 2-30 Celsius C. Emela ihe. Ejila ụbọchị mmebi.
.De emepeghị obere akpa ahụ ruo mgbe ị dịla njikere ịme ule, a na-atụ aro ka a na-eme nyocha dị n'okpuru ebe a chọrọ (ọnọdụ okpomọkụ 40-90%) na nkeji iri isii enwere ike.
A na-eji nwayọ nwayọ mgbe a mepere ya.

Warningsdọ aka na ntị na akpachapụ anya
.The Kit ga-akara ma chekwaa ya pụọ ​​na mmiri.
.
.
.Da anaghị eji reserd resergent.
.Dlie abụghị ngwa ahịa n'etiti Kits na adịghị iche iche ..
.Na na-ejikwa kaadị ule na ngwa ọ bụla.
.Miche, oke nlele ma ọ bụ obere ihe nlele nwere ike ibute nsonaazụ.

LNnomi
.As na ihe ndị ọ bụla na-eme ihe na-eme ihe na-eme ihe na-arụ ọrụ, o nwere ike ime ihe mgbochi site na mgbochi mgbochi nke ụmụ mmadụ (HAMA) na ụdị. Specisens sitere n'aka ndị ọrịa natara nkwadebe nke nje mgbochi monoconosis ma ọ bụ ọgwụgwọ nwere ike ịnwe Hama. Ụdị ndị dị otú ahụ nwere ike ibute nsonaazụ ụgha ma ọ bụ ụgha.
.Ndị nnwale ahụ bụ naanị maka ntụle ụlọ ọgwụ, ekwesịghị ịbụ naanị ihe kpatara ya, nyocha ahụike, nzaghachi, mmeghachi omume, EpideMology na ozi ndị ọzọ .
.HIGHIGHIRSASIRSA BITERRURU EGO NA-EGO NA MARA EGO. Ọ nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe nlele ndị ọzọ dị ka mmiri na mmamịrị na wdg.

Njirimara arụmọrụ

REnerise

1.Hassan JH, et Ntinye na ọgwụ ọjọọ nke sitere na immunody nke sitere na immunody [j].
2.levinson ss.The ọdịdị nke antibodic na intermomilic na intermomiasy miner (j] .j nke immunoasy, 1992: 108-114.

Igodo iji gosiputa ihe:

	Na vitronic Victostic ngwaọrụ
	Mmewe
	Chekwaa na 2-30 ℃
	Ụbọchị mmebi
	Etula ya
	Ikpachara anya
	Pụọ ntuziaka maka iji

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