Ụkpụrụ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na conjugate nke BSA na 25- (OH) VD na mpaghara ule na ewu mgbochi oke bekee IgG na mpaghara nchịkwa. A na-ekpuchi pad ihe nrịbama site na akara fluorescence anti 25-(OH) VD mgbochi na oke bekee IgG tupu oge eruo. Mgbe a na-anwale sample, 25- (OH) VD na sample jikọtara na fluorescence akara mgbochi 25- (OH) VD mgbochi, na-etolite dịghịzi ngwakọta. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, The free fluorescent marker ga-ejikọta ya na 25- (OH) VD na membrane. The ịta nke 25- (OH) VD bụ adịghị mma mmekọrịta maka fluorescence mgbaàmà, na ịta nke 25- (OH) VD na sample nwere ike chọpụta site flu.

Usoro nnwale:

Biko gụọ akwụkwọ ntuziaka ọrụ akụrụngwa na ntinye ngwugwu tupu ịnwale.

1. Debe ihe niile reagents na sample na ụlọ okpomọkụ.
2. Mepee Portable Immune Analyzer (WIZ-A101), tinye nbanye paswọọdụ akaụntụ dị ka usoro ọrụ nke ngwá ọrụ si dị, ma tinye interface nchọpụta ahụ.
3. Nyochaa koodu nyocha iji kwado ihe nnwale ahụ.
4. Wepụ kaadị ule na akpa foil.
5. Tinye kaadị ule n'ime oghere kaadị, nyochaa koodu QR, ma chọpụta ihe nlele ahụ.
6. Tinye 15μL serum ma ọ bụ plasma sample n'ime ngwọta, na mix ọma
7. Tinye ngwakọta 80μL na nlele nke ọma nke kaadị ahụ.
8. Pịa bọtịnụ "ọkọlọtọ ule", mgbe nkeji iri na ise gachara, ngwa ahụ ga-achọpụta kaadị ule na-akpaghị aka, ọ nwere ike gụọ nsonaazụ sitere na ihuenyo ngosipụta nke ngwaọrụ ahụ, wee dekọọ / bipụta nsonaazụ ule.
9. Rụtụ aka na ntuziaka nke Portable Immune Analyzer(WIZ-A101).