Ngwa nyocha(LATEX)maka Rotavirus Group A
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji
Kit diagnostic (LATEX) maka Rotavirus Group A dabara adaba maka nchọpụta qualitative nke Rotavirus Group A antigen n'ime nlele fecal mmadụ. Ezubere ule a maka naanị ndị ọkachamara ahụike. Ka ọ dị ugbu a, a na-eji ule a maka nyocha ụlọ ọgwụ nke afọ ọsịsa ụmụaka na ndị ọrịa nwere ọrịa Rotavirus Group A.

Nkwakọ ngwaahịa
1 ngwa / igbe, 10 ngwa / igbe, 25 ngwa, / igbe, 50 ngwa / igbe.

Nchịkọta
A na-ekewa Rotavirus dị ka arotavirusgenus nke nje exenteral, nke nwere udi okirikiri nke nwere dayameta nke ihe dịka 70nm. Rotavirus nwere akụkụ iri na otu nke RNA nwere eriri abụọ. Rotavirus nwere ike ịbụ otu asaa (ag) dabere na ọdịiche antigenic na njirimara mkpụrụ ndụ ihe nketa. A kọwo ọrịa nje nke otu A, B na C otu rotavirus. Rotavirus Group A bụ ihe dị mkpa na-ebute ọrịa gastroenteritis siri ike na ụmụaka n'ụwa nile^[1-2].

Usoro ASsay
1.Wepụ osisi sampling, tinye n'ime ihe nlele nke nsị, wee tinye osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, megharịa ihe ahụ ugboro 3. Ma ọ bụ na-eji osisi sampling were ihe dị ka 50mg faeces sample, na-etinye n'ime a feces sample tube nwere sample dilution, na ịghasa tightly.
2.Use disposable pipette sampling were the thinner feeces sample si onye ọrịa afọ ọsịsa, wee tinye 3 tụlee (ihe dị ka 100uL) na fecal sampling tube ma maa jijiji nke ọma, wepụ ya.
3.Wepụ kaadị ule ahụ site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.
4.Wepụ okpu site na tube sample na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.
5.N'ihi kwesịrị ịgụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.