Ngwa nyocha (LATEX) maka Antigen ka Helicobacter Pylori
Ngwa nyocha(LATEX)maka Antigen ka Helicobacter Pylori
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.
Ezubere iji
Ngwa nyocha (LATEX) maka Antigen ka Helicobacter Pylori dabara adaba maka ọnụnọ nke antigen H. Pylori n'ime nlele fecal mmadụ. Ezubere ule a maka naanị ndị ọkachamara ahụike. Ka ọ dị ugbu a, a na-eji ule a maka nyocha ụlọ ọgwụ nke afọ ọsịsa ụmụaka na ndị ọrịa nwere ọrịa HP.
Nkwakọ ngwaahịa
1 ngwa / igbe, 10 ngwa / igbe, 25 ngwa, / igbe, 50 ngwa / igbe.
Nchịkọta
Ọrịa H.pylori na gastritis na-adịghị ala ala, ọnya afọ, gastric adenocarcinoma, gastric mucosa jikọtara lymphoma nwere njikọ chiri anya, na gastritis, ọnya afọ, ọnyá afọ duodenal na ọrịa cancer gastric na ndị ọrịa nwere ọrịa H.pylori dị ihe dịka 90%. Òtù ahụike ụwa depụtara HP dị ka ụdị ọrịa carcinogen nke mbụ na nke doro anya na ọ bụ ihe ize ndụ maka ọrịa kansa afọ. Nchọpụta HP bụ ụzọ dị mkpa e si achọpụta ọrịa HP[1]. Ngwa ahụ bụ nchọpụta qualitative dị mfe na nke nwere ọgụgụ isi, na-achọpụta helicobacter pylori na nsị mmadụ, nke nwere nghọta dị elu na nkọwa siri ike. Enwere ike nweta nsonaazụ ya n'ime nkeji iri na ise dabere na nkọwapụta dị elu nke ụkpụrụ mmeghachi omume Sanwichi mgbochi mmadụ na usoro nyocha emulsion immunochromatography.
Usoro ASsay
1.Wepụ osisi sampling, tinye n'ime ihe nlele nke nsị, wee tinye osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, megharịa ihe ahụ ugboro 3. Ma ọ bụ na-eji osisi sampling were ihe dị ka 50mg faeces sample, na-etinye n'ime a feces sample tube nwere sample dilution, na ịghasa tightly.
2.Jiri ihe atụfu pipette sampling were ihe dị gịrịgịrị nsị n'aka onye ọrịa afọ ọsịsa, wee tinye dobe 3 (ihe dịka 100µL) na tube sampling fecal wee maa jijiji nke ọma, wepụ ya.
3.Wepụ kaadị ule ahụ site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.
4.Wepụ okpu site na tube sample na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.
5.N'ihi kwesịrị ịgụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.