Ngwa nyocha (Colloidal Gold) maka Transferrin
Ngwa nyocha(Colloidal Gold)maka Transferrin
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.
Ezubere iji
Kit diagnostic (Colloidal Gold) maka Transferrin (Tf) bụ nyocha ọla edo colloidal immunochromatographic maka nyocha qualitative nke Tf sitere na nsị mmadụ, ọ na-arụ ọrụ dị ka onye na-achọpụta ihe na-achọpụta ọbara ọgbụgba na eriri afọ. Teat bụ reagent nyocha, ihe nlele niile dị mma ga-ekwenyerịrị site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike. Ka ọ dị ugbu a, a na-eji ule a eme ihe maka IVD, ọ dịghị mkpa ọzọ ngwá ọrụ.
Nkwakọ ngwaahịa
1 ngwa / igbe, 10 ngwa / igbe, 25 ngwa, / igbe, 50 ngwa / igbe
Nchịkọta
Tf kachasị dị na plasma, nkezi ọdịnaya bụ ihe dịka 1.20 ~ 3.25g / L. N'ime afọ ndị ahụ siri ike, ọ fọrọ nke nta ka ọ bụrụ enweghị ọnụnọ. Mgbe tract digestive na-agba ọbara, Tf na serum na-abanye n'ime eriri afọ nke eriri afọ wee wepụ ya na nsị, ọ na-ejupụta na nsị nke ndị ọrịa na-agba ọbara nke eriri afọ. Ya mere, fecal Tf na-arụ ọrụ dị mkpa na nke dị mkpa maka nchọpụta ọbara ọgbụgba nke eriri afọ. Ngwa ahụ bụ ule dị mfe, nke na-ahụ anya nke na-achọpụta Tf na nsị mmadụ, ọ nwere mmetụta nchọpụta dị elu na nkọwa siri ike. Nnwale ahụ dabere na ụkpụrụ mmeghachi omume Sanwichi okpukpu abụọ dị elu yana teknụzụ nyocha nyocha nke immunochromatographic ọla edo, ọ nwere ike inye nsonaazụ n'ime nkeji iri na ise.
Usoro ASsay
1.Wepụ osisi sampling, tinye n'ime ihe nlele nke nsị, wee tinye osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, megharịa ihe ahụ ugboro 3. Ma ọ bụ na-eji osisi sampling were ihe dị ka 50mg faeces sample, na-etinye n'ime a feces sample tube nwere sample dilution, na ịghasa tightly.
2.Use disposable pipette sampling were the thinner feeces sample si onye ọrịa afọ ọsịsa, wee tinye 3 tụlee (ihe dị ka 100uL) na fecal sampling tube ma maa jijiji nke ọma, wepụ ya.
3.Wepụ kaadị ule ahụ site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.
4.Wepụ okpu site na tube sample na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.
5.N'ihi kwesịrị ịgụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.