Ngwa nyocha(Colloidal Gold)maka Calprotectin
N'ihi na in vitro diagnostic ojiji naanị
Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji
Ngwa nyocha maka Calprotectin (cal) bụ nyocha immunochromatographic ọla edo nke colloidal maka mkpebi siri ike nke cal sitere na nsị mmadụ, nke nwere uru nrịbama ngwa dị mkpa maka ọrịa afọ ọzịza. Nnwale a bụ reagent nyocha. A ga-akwadorịrị nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike. Ka ọ dị ugbu a, a na-eji ule a eme ihe maka IVD, ọ dịghị mkpa ọzọ ngwá ọrụ.

Nchịkọta
Cal bụ heterodimer, nke mejupụtara MRP 8 na MRP 14. Ọ dị na cytoplasm neutrophils ma gosipụta ya na membranes cell mononuclear. Cal bụ protein na-agba agba, ọ nwere usoro kwụsiri ike nke ọma ihe dị ka otu izu n'ime afọ mmadụ, e kpebisiri ike na ọ ga-abụ akara nrịanrịa na-afụ ụfụ. Ngwa ahụ bụ ule dị mfe, nke na-ahụ maka ọkara nke na-achọpụta cal n'ime afọ mmadụ, ọ nwere mmetụta nchọpụta dị elu yana nkọwa siri ike. Nnwale ahụ dabere na ụkpụrụ mmeghachi omume Sanwichi okpukpu abụọ dị elu yana teknụzụ nyocha nyocha nke immunochromatographic ọla edo, ọ nwere ike inye nsonaazụ n'ime nkeji iri na ise.

Ụkpụrụ nke usoro
The warara nwere mgbochi cal mkpuchi McAb na ule mpaghara na ewu mgbochi oke bekee IgG mgbochi na mpaghara njikwa, nke na-agbakwunyere na akpụkpọ ahụ chromatography tupu oge eruo. Ejiri ọla edo colloidal nke akpọrọ anti cal McAb na kolloidal ọla edo akpọrọ oke bekee IgG mgbochi tupu oge eruo. Mgbe a na-anwale nlele dị mma, cal dị na sample na-abịa na ọla edo colloidal nke akpọrọ anti cal McAb, wee mepụta mgbagwoju anya na-alụso ọrịa ọgụ, ebe a na-ahapụ ya ka ọ kwaga n'akụkụ ebe nnwale ahụ, ihe mgbochi cal conjugate na-ejide McAb na akpụkpọ ahụ na ụdị. Mgbakwụnye "mgbochi cal McAb-cal-colloidal gold nke akpọrọ anti cal McAb" mgbagwoju, eriri ule nwere agba pụtara na mpaghara ule. Agba agba na-ejikọta nke ọma na ọdịnaya cal. Ihe nlele na-adịghị mma anaghị ewepụta eriri ule n'ihi enweghị mgbagwoju anya colloidal gold conjugate cal. N'agbanyeghị cal dị na sample ma ọ bụ na ọ bụghị, enwere eriri uhie na-apụta na mpaghara ntụaka na mpaghara njikwa mma, nke a na-ahụta dị ka ụkpụrụ ụlọ ọrụ dị mma.

Reagent na ihe ndị e nyere
Ngwa ngwugwu 25T:
.Kaadị ule n'otu n'otu n'otu n'otu foil nke e ji ihe eji akwọcha akpa juru
.Sample diluents: Efrata bụ 20mM pH7.4PBS
.Dispete
.Ngwunye ntinye

AKWỤKWỌ KWESỊRỊ MA Ọ BỤGHỊ

Nlereanya mkpokọta akpa, ngụ oge

NKWUKWU NA NKWUKWU
Jiri akpa dị ọcha nke a na-atụfu ihe ga-anakọta ihe nlele nsị ọhụrụ, wee nwalee ozugbo. Ọ bụrụ na enweghị ike ịnwale ozugbo, biko chekwaa na 2-8°C maka awa 12 ma ọ bụ ala -15°C maka ọnwa anọ.

Usoro ASsay
1.Wepụ osisi sampling, tinye n'ime ihe nlele nke nsị, wee tinye osisi ahụ azụ azụ, kpochie ya ma maa jijiji nke ọma, megharịa ihe ahụ ugboro 3. Ma ọ bụ na-eji ihe nlele ahụ etinyere ihe dị ka 50mg faeces sample, ma tinye n'ime tube nke nwere nsị sampị nke nwere ihe nzacha sampị, ma kpochie ya nke ọma.
2.Use disposable pipette sampling were the thinner feeces sample si onye ọrịa afọ ọsịsa, wee tinye 3 tụlee (ihe dị ka 100uL) na fecal sampling tube ma maa jijiji nke ọma, wepụ ya.
3.Wepụ kaadị ule ahụ site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.
4.Wepụ okpu site na tube sample na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample verticaly na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette, malite oge.
5.N'ihi kwesịrị ịgụ n'ime 10-15 nkeji, na ọ bụ adịghị irè mgbe 15 nkeji.

Nsonaazụ ule na ntụgharị asụsụ

Nchekwa na Nkwụsi ike
Ngwa ahụ bụ ndụ ọnwa 24 site na ụbọchị arụpụtara ya. Chekwaa ngwa ndị a na-ejighị ya na 2-30 Celsius. Emepela obere akpa ahụ emechiri emechi ruo mgbe ị dị njikere ime ule.
Ịdọ aka ná ntị na ịkpachara anya
1.The kit kwesịrị akara na-echebe megide mmiri mmiri¹.
2.Una eji sample nke a na-etinye ogologo oge ma ọ bụ ugboro ugboro kefriza na thawing iji nwalee
3.Fecal samples bụ oke ma ọ bụ ọkpụrụkpụ nwere ike ime ka diluted samples rụrụ arụ ule kaadị, biko centrifuge diluted sample na-ewere supernatant maka ule.
4.Misoperation, oke ma ọ bụ obere sample nwere ike iduga n'ihi ọdịiche.

MAKA
1.Nke a ule N'ihi bụ naanị maka ụlọ ọgwụ kwuru, ekwesịghị ije ozi dị ka nanị ihe ndabere maka clinical nchoputa na ọgwụgwọ, ndị ọrịa clinical management kwesịrị keukwu echiche jikọtara ya mgbaàmà, ọgwụ akụkọ ihe mere eme, ndị ọzọ laabu nnyocha, ọgwụgwọ nzaghachi, ọrịa na ndị ọzọ. ozi².
2.This reagent na-eji naanị maka fecal ule. O nwere ike ọ gaghị enweta nsonaazụ ziri ezi mgbe ejiri ya mee ihe maka nlele ndị ọzọ dị ka mmiri mmiri na mmamịrị na wdg.