Ngwa nyocha maka Procalcitonin

(immunochromatographic nyocha nke fluorescence)

N'ihi na in vitro diagnostic ojiji naanị

Biko gụọ ihe ngwungwu a nke ọma tupu ejiri ya wee soro ntuziaka ahụ. Enweghị ike ịkwenye ntụkwasị obi nke nsonaazụ nyocha ma ọ bụrụ na ọ dị iche na ntuziaka dị na ntinye ngwugwu a.

Ezubere iji

Ngwa nyocha maka Procalcitonin (fluorescence immunochromatographic assay) bụ fluorescence immunochromatographic assay maka nchọpụta quantitative nke Procalcitonin (PCT) na ọbara mmadụ ma ọ bụ plasma, a na-eji ya maka nyocha inyeaka nke ọrịa nje na sepsis. A ga-akwadorịrị nlele niile dị mma site na usoro ndị ọzọ. Ezubere ule a maka naanị ndị ọkachamara ahụike.

Nchịkọta

Procalcitonin nwere amino acid 116 na ịdị arọ ya bụ 12.7KD. A na-egosipụta PCT site na mkpụrụ ndụ neuroendocrine wee gbarie site na enzymes n'ime (akabeghị aka) calcitonin, peptide carboxy-terminating, na amino terminating peptide. Ndị nwere ahụike nwere naanị obere PCT n'ọbara ha, nke nwere ike ịba ụba nke ukwuu mgbe ọrịa nje gasịrị. Mgbe sepsis na-eme n'ime ahụ, ọtụtụ anụ ahụ nwere ike igosipụta PCT, yabụ enwere ike iji PCT mee ihe dị ka ihe ngosipụta nke sepsis. Maka ụfọdụ ndị ọrịa nwere ọrịa mbufụt, PCT nwere ike iji dị ka ihe na-egosi nhọrọ ọgwụ nje na mkpebi dị mma.

Ụkpụrụ nke usoro

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na mgbochi PCT na mpaghara ule yana ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi PCT antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, PCT antigen dị na sample jikọtara ya na fluorescence akpọrọ mgbochi PCT, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ, mgbe mgbagwoju gafere ule mpaghara, ọ jikọtara ya na mgbochi PCT mkpuchi mgbochi, na-etolite ọhụrụ mgbagwoju. A na-ejikọta ọkwa PCT nke ọma na mgbama fluorescence, na ntinye nke PCT na nlele nwere ike chọpụta site na fluorescence immunoassay assay.