Ụkpụrụ

A na-ekpuchi akpụkpọ ahụ nke ngwaọrụ ule na Tf antibody na mpaghara ule yana ewu mgbochi oke bekee IgG na mpaghara njikwa. A na-ekpuchi pad lable site na fluorescence akpọrọ mgbochi Tf antibody na oke bekee IgG tupu oge eruo. Mgbe a na-anwale nlele dị mma, Tf antigen dị na sample na-ejikọta ya na fluorescence akpọrọ anti Tf antibody, wee mepụta ngwakọta mgbochi. N'okpuru omume nke immunochromatography, mgbagwoju eruba na ntụziaka nke absorbent akwụkwọ. Mgbe mgbagwoju gafere mpaghara ule, ọ jikọtara ya na mgbochi mgbochi Tf mkpuchi, na-etolite mgbagwoju ọhụrụ.

Ọ bụrụ na ọ dị njọ, ihe nlele ahụ enweghị transferrin ma ọ bụ ọdịnaya dị ala, nke mere na enweghi ike ịmepụta mgbagwoju anya. Agaghị enwe ahịrị uhie na mpaghara nchọpụta (T). N'agbanyeghị ma Tf antibody dị na ụdịdị ahụ ma ọ bụ na ọ nọghị, ihe mgbochi colloidal ọla edo nke fọdụrụ na-emegide mmadụ IgM monoclonal antibody na ewu mgbochi òké IgG mgbochi mkpuchi na mpaghara njikwa mma (C). Mgbe ahụ agglutinates na-etolite agba na mpaghara njikwa mma, na ahịrị uhie ga-apụta na (C). Ahịrị uhie bụ ọkọlọtọ na-egosi na mpaghara njikwa mma (C) maka ikpebi ma enwere ihe nlele zuru oke yana ma usoro chromatography ọ bụ nkịtị. A na-ejikwa ya dị ka ụkpụrụ njikwa ime maka reagents.

Usoro ule

Biko gụọ ihe ntinye ngwugwu tupu ịnwale.

1.Wepụ kaadị ule site na akpa foil, tinye ya na tebụl larịị ma kaa ya akara.

2.Wepụ okpu si sample tube na-atụfu akpa abụọ tụlee diluted sample, tinye 3 tụlee (banyere 100uL) ọ dịghị afụ diluted sample kwụ ọtọ na nwayọọ nwayọọ n'ime sample ọma nke kaadị na nyere dispette. Mgbe ahụ malite ngụ oge.