PRINCIPLE

Cov sawb muaj cov tshuaj tiv thaiv cal txheej McAb ntawm thaj chaw kuaj thiab tshis los tiv thaiv luav IgG tshuaj tiv thaiv ntawm cheeb tsam tswj, uas yog fastened rau daim nyias nyias chromatography ua ntej. Lable pad yog coated los ntawm colloidal kub sau los tiv thaiv cal McAb thiab colloidal kub sau luav IgG antibody ua ntej. Thaum kuaj cov qauv zoo, cov cal nyob rau hauv cov qauv tuaj nrog colloidal kub sau los tiv thaiv cal McAb, thiab daim ntawv tiv thaiv kab mob, raws li nws tau tso cai rau tsiv teb tsaws raws li qhov kev xeem strip, cal conjugate complex yog ntes los ntawm anti cal txheej McAb ntawm daim nyias nyias thiab daim ntawv. "anti cal coating McAb-cal-colloidal gold labeled anti cal McAb" complex, ib pawg kuaj xim xim tau tshwm sim hauv cheeb tsam xeem. Cov xim siv tau zoo sib xws nrog cov ntsiab lus cal. Ib qho piv txwv tsis zoo tsis tsim ib qho kev sim qhab vim tsis muaj colloidal kub conjugate cal complex. Tsis muaj teeb meem cal yog tam sim no nyob rau hauv cov qauv los yog tsis, muaj ib tug liab kab txaij tshwm sim nyob rau hauv reference cheeb tsam thiab zoo tswj cheeb tsam, uas yog suav hais tias raws li zoo nyob rau hauv lub enterprise qauv.

Cov txheej txheem xeem:

1. Tshem tawm cov sampling stick, muab tso rau hauv cov qauv faeces, ces muab cov sampling stick rov qab, ntsia hlau nruj thiab co zoo, rov ua dua 3 zaug. Los yog siv cov sampling stick khaws txog 50mg faeces qauv, thiab muab tso rau hauv ib lub faeces qauv raj uas muaj cov qauv dilution, thiab ntswj kom nruj.

2. Siv cov khoom siv pov tseg pov tseg coj cov qauv faeces thinner los ntawm tus neeg mob raws plab, tom qab ntawd ntxiv 3 tee (kwv yees li 100uL) rau hauv lub raj fecal sampling tube thiab co zoo, muab tso tseg.

3. Tshem tawm daim ntawv xeem los ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj theem thiab kos nws.

4. Tshem lub hau ntawm cov qauv raj thiab muab pov tseg thawj ob tee diluted qauv, ntxiv 4.drops (txog 100uL) tsis muaj npuas diluted qauv verticaly thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette, pib lub sijhawm.

5.Qhov tshwm sim yuav tsum tau nyeem nyob rau hauv 10-15 feeb, thiab nws yog invalid tom qab 15 feeb