TSAB NTAWV CAI

Daim kab xev muaj MP-Ag txheej antigen ntawm thaj chaw kuaj thiab tshis tiv thaiv nas IgG antibody ntawm thaj chaw tswj, uas tau khi rau daim nyias nyias chromatography ua ntej. Daim ntawv lo yog coated los ntawm colloidal kub labeled nas-anti tib neeg IgM McAb ua ntej. Thaum kuaj cov qauv zoo, MP-IgM hauv cov qauv sib xyaw nrog colloidal kub labeled nas-anti tib neeg IgM McAb, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov tshuaj thiab cov qauv sab hauv ntawm daim nyias nyias nitrocellulose ntws mus rau hauv kev coj ntawm daim ntawv nqus dej, thaum cov tshuaj dhau thaj chaw kuaj, nws sib xyaw nrog MP-Ag txheej antigen, tsim "MP-Ag txheej antigen-MP-IgM-colloidal kub labeled nas-anti tib neeg IgM McAb" complex, ib daim kab xev kuaj xim tshwm sim ntawm thaj chaw kuaj. Cov qauv tsis zoo tsis tsim cov kab xev kuaj vim tsis muaj zog tiv thaiv kab mob. Txawm hais tias MP-IgM muaj nyob hauv cov qauv lossis tsis muaj, muaj ib daim kab xev liab tshwm sim ntawm thaj chaw tswj xyuas zoo, uas suav tias yog cov qauv zoo hauv tuam txhab.

Cov Txheej Txheem Xeem:

Cov txheej txheem xeem WIZ-A101 saib cov lus qhia ntawm lub tshuab ntsuas kev tiv thaiv kab mob portable. Cov txheej txheem kuaj pom yog raws li nram no:

1. Muab tag nrho cov tshuaj reagents thiab cov qauv tso rau qhov kub thiab txias hauv chav.
2. Tshem daim ntawv xeem tawm ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj sib dhos thiab cim nws.
3. Ntxiv 10μL serum los yog plasma qauv los yog 20μL tag nrho cov ntshav qauv rau qauv zoo ntawm daim npav nrog muab dispette, ces ntxiv 100μL (kwv yees li 2-3 tee) qauv diluent, pib lub sijhawm.
4. Tos tsawg kawg yog 10-15 feeb thiab nyeem qhov tshwm sim, qhov tshwm sim yuav tsis siv tau tom qab 15 feeb.