PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj tau coated nrog Pab Pawg A thiab adenovirus antigen ntawm thaj chaw kuaj thiab tshis anti luav IgG antibody ntawm thaj tsam tswj. Lable pad yog coated los ntawm fluorescence sau los tiv thaiv pawg A thiab adenovirus thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo rau Pawg A thiab adenovirus, Pab Pawg A thiab adenovirus hauv cov qauv ua ke nrog cov tshuaj fluorescence sau npe tiv thaiv Rotavirus Pawg A thiab adenovirus, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev txiav txim ntawm immunochromatography, complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv. Thaum txoj kev dhau los ntawm cheeb tsam xeem, nws ua ke nrog cov tshuaj tiv thaiv Rotavirus Group A thiab adenovirus txheej antibody, tsim cov complex tshiab. Yog tias nws tsis zoo, tsis muaj Rotavirus Group A thiab adenovirus antigen hauv cov qauv, yog li tsis tuaj yeem tsim cov tshuaj tiv thaiv kab mob, yuav tsis muaj kab liab hauv cheeb tsam kuaj pom (T). Txawm hais tias pawg A rotavirus thiab adenovirus muaj nyob rau hauv cov qauv, cov latex-labeled nas IgG yog chromatographed rau thaj tsam tswj zoo (C) thiab ntes los ntawm tshis anti-nas IgG antibody. Ib kab liab yuav tshwm sim hauv cheeb tsam tswj kom zoo (C). Cov kab liab yog tus qauv tshwm nyob rau hauv cheeb tsam tswj qhov zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas zoo li qub. Nws kuj tseem siv los ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov txheej txheem xeem:

1. Qhib lub hau ntawm lub raj coj mus kuaj. Tsis txhob nchuav cov tshuaj rau hauv lub raj mis.
2. Tshem tawm cov sampling stick, muab tso rau hauv lub faeces qauv (los yog siv ib tug sampling stick los khaws li 50mg ntawm quav), ces muab cov sampling stick rov qab, ntsia hlau nruj thiab co zoo, rov ua 3 zaug. Siv ib feem sib txawv ntawm tus qauv faeces txhua zaus. Tom qab sampling, muab tus sampling pas nrig rau hauv lub faeces collection tube uas muaj cov qauv diluent, thiab ntsia hlau dropper kom nruj. Yog tias cov quav hniav ntawm tus neeg mob raws plab yog thinner, siv cov straw yas pov tseg tuaj yeem siv los kuaj. Siv cov khoom siv pov tseg pov tseg coj cov qauv faeces thinner los ntawm tus neeg mob raws plab, tom qab ntawd ntxiv 3 tee (kwv yees li 100uL) rau hauv lub raj fecal sampling.
3. Co cov qauv zoo thiab tshem tawm lub hau ntawm lub taub hau thiab muab tso rau ib sab.
4. Thaum khaws cia ntawm qhov kub thiab txias, cov khoom siv yuav tsum rov qab los rau hauv chav sov ua ntej siv. Tshem tawm daim npav xeem los ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj theem thiab kos nws.
5. Tshem lub hau los ntawm cov qauv raj thiab muab pov tseg thawj ob tee diluted qauv, ntxiv 3 tee (li 100uL) tsis muaj npuas diluted qauv vertically thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette, pib lub sijhawm.
6. Cov txiaj ntsig yuav tsum tau nyeem tsis pub dhau 10-15 feeb, thiab nws tsis raug cai tom qab 15 feeb.