TSAB NTAWV CAI

Lub membrane ntawm lub cuab yeej kuaj tau coated nrog Hp-Ab antibody rau ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG antibody rau ntawm thaj chaw tswj. Lub hau npog tau coated los ntawm fluorescence labeled anti Hp-Ag thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, Hp-Ab hauv cov qauv sib xyaw nrog fluorescence labeled anti Hp-Ag, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov complex ntws mus rau hauv qhov kev taw qhia ntawm daim ntawv nqus dej. Thaum complex dhau thaj chaw kuaj, nws ua ke nrog antibody txheej anti Hp-Ag, tsim cov complex tshiab. Yog tias nws tsis zoo, tsis muaj HP antibody hauv cov qauv, yog li ntawd cov tshuaj tiv thaiv kab mob tsis tuaj yeem tsim, yuav tsis muaj kab liab hauv thaj chaw kuaj pom (T). Kab liab yog tus qauv tshwm sim hauv thaj chaw tswj xyuas zoo (C) rau kev txiav txim siab seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas yog ib txwm muaj. Nws kuj tseem siv ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov Txheej Txheem Xeem:

Thov nyeem daim ntawv qhia ua ntej sim.

1. Tshem daim ntawv xeem tawm ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj sib dhos thiab cim nws.

2. Ntxiv 2 tee ntawm cov qauv serum lossis plasma (lossis 3 tee ntawm cov ntshav tag nrho / cov ntshav ntawm tus ntiv tes) rau hauv qhov dej ntawm daim npav nrog lub raj mis uas muab, tom qab ntawd ntxiv 1 tee ntawm cov qauv diluent, pib lub sijhawm.

3. Tos tsawg kawg yog 10-15 feeb thiab nyeem qhov tshwm sim hauv 10-15 feeb. Qhov tshwm sim yuav tsis siv tau tom qab 15 feeb.