LUS QHIA

Vitamin D yog ib qho vitamin thiab kuj yog ib qho tshuaj hormones steroid, feem ntau suav nrog VD2 thiab VD3, uas nws cov qauv zoo sib xws heev. Vitamin D3 thiab D2 raug hloov mus ua 25 hydroxyl vitamin D (suav nrog 25-dihydroxyl vitamin D3 thiab D2). 25-(OH) VD hauv tib neeg lub cev, kev tsim kho ruaj khov, muaj kev sib xyaw siab. 25-(OH) VD qhia txog tag nrho cov vitamin D, thiab lub peev xwm hloov pauv ntawm vitamin D, yog li 25-(OH) VD raug suav hais tias yog qhov qhia zoo tshaj plaws rau kev ntsuam xyuas qib ntawm vitamin D. Lub Khoom Siv Kuaj Mob yog raws li immunochromatography thiab tuaj yeem muab cov txiaj ntsig hauv 15 feeb.

TSAB NTAWV QHIA TXOG TXHEEJ TXHEEM

Lub membrane ntawm lub cuab yeej kuaj tau coated nrog conjugate ntawm BSA thiab 25-(OH)VD ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG antibody ntawm thaj chaw tswj. Lub cim ncoo tau coated los ntawm fluorescence cim tiv thaiv 25-(OH)VD antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv, 25-(OH)VD hauv cov qauv ua ke nrog fluorescence cim tiv thaiv 25-(OH)VD antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov dej ntws hauv qhov kev taw qhia ntawm daim ntawv nqus, thaum complex dhau thaj chaw kuaj, Lub cim fluorescent dawb yuav raug ua ke nrog 25-(OH)VD ntawm daim nyias nyias. Qhov concentration ntawm 25-(OH)VD yog qhov tsis zoo rau lub teeb liab fluorescence, thiab qhov concentration ntawm 25-(OH)VD hauv cov qauv tuaj yeem pom los ntawm fluorescence immunoassay assay.