TSEEM CEEB

Vitamin D yog cov vitamin thiab tseem yog cov tshuaj steroid, feem ntau suav nrog VD2 thiab VD3, nws txoj kev qhia zoo sib xws. Vitamin D3 thiab D2 hloov mus rau 25 hydroxyl vitamin D (xws li 25-dihydroxyl vitamin D3 thiab D2). 25-(OH) VD nyob rau hauv tib neeg lub cev, ruaj khov kev qhia, siab concentration. 25-(OH) VD qhia txog tag nrho cov vitamin D, thiab lub peev xwm hloov pauv ntawm cov vitamin D, yog li 25-(OH)VD raug suav hais tias yog qhov qhia tau zoo tshaj plaws rau kev ntsuas qib ntawm cov vitamin D.Cov khoom siv kuaj mob yog raws li. immunochromatography thiab tuaj yeem muab qhov tshwm sim hauv 15 feeb.

TSEV KAWM NTAWV NO

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog lub conjugate ntawm BSA thiab 25-(OH)VD ntawm thaj tsam xeem thiab tshis anti luav IgG antibody ntawm thaj tsam tswj. Marker ncoo yog coated los ntawm fluorescence mark anti 25-(OH)VD antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv, 25-(OH)VD hauv cov qauv ua ke nrog fluorescence cim antibody 25-(OH)VD, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev txiav txim ntawm lub immunochromatography, cov complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv, thaum complex dhau lub xeem cheeb tsam, lub dawb fluorescent marker yuav ua ke nrog 25-(OH)VD ntawm daim nyias nyias.Qhov concentration ntawm 25-(OH) VD yog qhov tsis zoo rau kev sib raug zoo rau cov teeb liab fluorescence, thiab qhov concentration ntawm 25-(OH)VD hauv cov qauv tuaj yeem kuaj pom los ntawm fluorescence immunoassay assay.