TSAB NTAWV CAI

Lub membrane ntawm lub cuab yeej kuaj tau coated nrog microalbumin antigen ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG antibody ntawm thaj chaw tswj. Lub hau npog tau coated los ntawm fluorescence labeled microalbumin thiab luav IgG ua ntej. Yog tias tsis muaj albumin hauv cov zis, cov colloidal kub-labeled anti-Alb-labeled monoclonal antibody ntawm daim ntawv kub colloidal yuav khiav ntawm daim membrane nrog cov zis mus rau kab kuaj pom, thiab ua ke nrog Alb-coated antigen nrog kab pom. Thiab cov xim kab tsaus dua li cov xim kab hauv thaj chaw tswj (C), qhov no yog qhov tshwm sim tsis zoo. Yog tias cov zis muaj albumin, lawv yuav sib tw nrog Alb-coated antigen ntawm daim membrane kom khi rau cov chaw antibody txwv ntawm colloidal kub-labeled anti-Alb-labeled monoclonal antibody. Raws li qhov ntau ntawm albumin hauv cov zis nce ntxiv, kev kuaj

Cov xim ntawm kab yuav dhau los ua xim sib dua thiab sib dua. Cov ntsiab lus ntawm albumin hauv cov zis tuaj yeem pom tau ib nrab los ntawm kev sib piv thaj chaw kuaj pom (T) nrog thaj chaw tswj (C). Thaj chaw tswj xyuas zoo (C) thiab thaj chaw siv (R) ntawm cov khoom siv yuav ib txwm tshwm sim thaum lub sijhawm kuaj, thiab tsis muaj dab tsi ua rau muaj cov albumin hauv cov zis. Thaj chaw tswj (C) thiab thaj chaw siv (R) kab tuaj yeem siv ua cov ntsuas kev tswj xyuas zoo sab hauv rau cov khoom siv.

Cov Txheej Txheem Xeem:

Thov nyeem phau ntawv qhia siv cov cuab yeej thiab cov ntawv sau rau hauv pob ua ntej kuaj. Yaj cov qauv mus rau qhov kub hauv chav ua ntej siv.

1. Tshem daim ntawv xeem tawm ntawm lub hnab ntawv ci. Muab tso rau ntawm qhov chaw tiaj tus thiab cim.

2. Siv lub raj mis pov tseg los ntawm kev siv cov zis coj mus kuaj, pov tseg ob tee dej zis thawj zaug. Ntxiv 3 tee dej (kwv yees li 100uL) ntawm cov zis uas tsis muaj npuas rau hauv nruab nrab ntawm lub qhov coj mus kuaj ntawm daim npav kuaj ntsug thiab pib lub sijhawm.

3. Nyeem cov txiaj ntsig hauv 10-15 feeb. Tsis siv tau yog tias ntau tshaj 15 feeb.