Lub ntsiab lus

Daim nyias nyias ntawm cov cuab yeej ntsuas yog coated nrog anti EV71 tshuaj tiv thaiv ntawm thaj tsam xeem thiab tshis cov tshuaj tiv thaiv ntawm cheeb tsam tswj. Lable Pad yog coated los ntawm fluorescence sau los tiv thaiv ERI71 ovribody thiab luav igg ua ntej. Thaum kuaj cov qauv zoo, AIR71 antigen hauv cov qauv ua ke nrog fluorescence los tiv thaiv AV71 tshuaj tiv thaiv kab mob tiv thaiv, thiab kev sib xyaw tiv thaiv kab mob tiv thaiv kab mob, thiab tshuaj tiv thaiv kab mob tiv thaiv kab mob, thiab kev sib xyaw ua paug. Raws li kev txiav txim ntawm cov chromatography, cov nyom ntws ntawm cov ntawv ntsuas, thaum ua ke nrog anti ev71 txheej tshuaj tiv thaiv antibody, cov ntaub ntawv tshiab.

Yog tias nws tsis zoo, cov qauv tsis muaj ENTERIIRUS 71 IGM tshuaj tiv thaiv, yog li cov tshuaj tiv thaiv kab mob tsis tuaj yeem tsim tau. Yuav tsis muaj txoj kab liab nyob hauv thaj chaw kuaj pom (t). Txawm hais tias itovirus 71 orgon antibdys muaj nyob rau hauv cov hnoos qeev uas tsis yog, tus tshis anti-a anti-mion anti-mion anti-anti-awg anti-awg antibody coated nyob rau hauv cov chaw tswj zoo (c) khi. Tom qab ntawd lub agglutinates tsim cov xim hauv qhov chaw tswj tau zoo, thiab txoj kab liab yuav tshwm sim hauv (c). Cov kab liab yog tus qauv tshwm nyob hauv thaj chaw tswj tau zoo (c) rau kev txiav txim seb puas muaj cov txheej txheem chromatogy yog qhov qub. Nws kuj tseem siv los ua tus qauv sab hauv rau cov reagents.

Ntsuam xyuas cov txheej txheem:

1.Qhov kev kuaj sim tuaj yeem kuaj xyuas tag nrho cov ntshav, suav nrog cov ntshav ntshav lossis ntshav ntshav. Tag nrho cov ntshav tsis tuaj yeem khaws cia tom qab sau. Kuv yuav tsum tau siv sai sai tom qab sau.

2.Serum kuaj tau sau tshuaj aspectically raws li cov qauv txheej txheem. Cov cua sov-inactivated tsis tuaj yeem siv tau. Nws tsis pom zoo kom siv cov lipemic, turbid lossis ntshav paug. Qhov teeb meem hauv ntshav ntshav. Thiab nag lossis daus yuav cuam tshuam cov txiaj ntsig kev sim, cov qauv no yuav tsum yog Centrifuged lossis lim ua ntej siv.

3.Qhov kev kuaj sim tuaj yeem ua tau Heprium, sodium citrate lossis Edta anticoagulant ntshav.

4.According rau cov qauv txheej txheem sau cov qauv. Ntshav dej lossis ntshav ntshav lossis cov qauv ntshav lossis cov qauv ntshav tuaj yeem khaws cia hauv tub yees ntawm 2-8 ℃ rau 3 hnub thiab cryopreservation hauv qab -15 ° C rau 3 lub hlis.

5.Oll qauv zam kev zam kom khov-thaw mus.