PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog anti EV71 antibody nyob rau hauv lub xeem cheeb tsam thiab tshis anti luav IgG antibody nyob rau hauv lub cheeb tsam tswj. Lable ncoo yog coated los ntawm fluorescence sau los tiv thaiv EV71 antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, EV71 antigen hauv cov qauv ua ke nrog cov tshuaj fluorescence sau tias antibody EV71, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev txiav txim ntawm cov chromatography, cov complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv, thaum complex dhau lub xeem cheeb tsam, nws ua ke nrog anti EV71 txheej antibody, cov ntaub ntawv tshiab complex.

Yog tias nws tsis zoo, tus qauv tsis muaj cov kab mob enterovirus 71 IgM antibody, kom lub cev tiv thaiv tsis tuaj yeem tsim. Yuav tsis muaj kab liab nyob rau hauv qhov chaw kuaj pom (T). Txawm hais tias Enterovirus 71 IgM cov tshuaj tiv thaiv muaj nyob rau hauv cov qauv los yog tsis, qhov seem ntawm cov tshuaj tiv thaiv kab mob colloidal kub-labeled nas anti-tib neeg IgM monoclonal antibody thiab tshis anti-nas IgG antibody coated nyob rau hauv qhov chaw tswj zoo (C) khi. Tom qab ntawd cov agglutinates tsim cov xim hauv cheeb tsam tswj kom zoo, thiab cov kab liab yuav tshwm hauv (C). Cov kab liab yog tus qauv tshwm nyob rau hauv cheeb tsam tswj qhov zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas zoo li qub. Nws kuj tseem siv los ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov txheej txheem xeem:

1.Cov qauv kuaj tuaj yeem yog cov ntshav tag nrho, suav nrog cov ntshav venous lossis ntshav Peripheral. Tag nrho cov ntshav tsis tuaj yeem khaws cia tom qab sau. Kuv yuav tsum tau siv sai tom qab sau.

2.Serum kuaj tau sau aseptically raws li cov txheej txheem txheej txheem. Heat-inactivated serum siv tsis tau. Nws tsis pom zoo kom siv lipemic, turbid los yog cov kab mob sib kis. Particulate teeb meem nyob rau hauv serum. Thiab nag lossis daus yuav cuam tshuam rau qhov kev sim, cov qauv no yuav tsum tau centrifuged lossis lim ua ntej siv.

3.Cov qauv kuaj tuaj yeem yog heparin, Sodium citrate lossis EDTA anticoagulant plasma.

4.Raws li cov txheej txheem txheej txheem sau cov qauv. Cov ntshav los yog cov ntshav ntshav tuaj yeem khaws cia hauv tub yees ntawm 2-8 ℃ rau 3 hnub thiab khaws cia hauv qab -15 ° C rau 3 lub hlis.

5.Txhua tus qauv tsis txhob khov-thaw mus.