TSAB NTAWV CAI

Lub membrane ntawm lub cuab yeej kuaj tau coated nrog antibody EV71 rau ntawm thaj chaw kuaj thiab tshis antibody IgG antibody rau ntawm thaj chaw tswj. Lub pad label tau coated los ntawm fluorescence labeled antibody EV71 thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, EV71 antigen hauv cov qauv sib xyaw nrog fluorescence labeled antibody EV71, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev ua ntawm chromatography, cov complex ntws hauv kev coj ntawm daim ntawv nqus, thaum complex dhau thaj chaw kuaj, nws ua ke nrog antibody EV71 txheej, tsim cov complex tshiab.

Yog tias nws tsis muaj, cov qauv kuaj tsis muaj enterovirus 71 IgM antibody, yog li ntawd cov tshuaj tiv thaiv kab mob tsis tuaj yeem tsim tau. Yuav tsis muaj kab liab hauv thaj chaw kuaj pom (T). Txawm hais tias Enterovirus 71 IgM antibody muaj nyob hauv cov qauv kuaj lossis tsis muaj, cov tshuaj tiv thaiv nas IgM monoclonal antibody uas muaj daim ntawv lo kub thiab cov tshuaj tiv thaiv tshis IgG antibody uas tau coated hauv thaj chaw tswj xyuas zoo (C) yuav khi. Tom qab ntawd cov agglutinates tsim xim hauv thaj chaw tswj xyuas zoo, thiab kab liab yuav tshwm sim hauv (C). Kab liab yog tus qauv tshwm sim hauv thaj chaw tswj xyuas zoo (C) rau kev txiav txim siab seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas yog ib txwm muaj. Nws kuj tseem siv ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov Txheej Txheem Xeem:

1. Cov qauv kuaj no yuav yog cov ntshav tag nrho, suav nrog cov ntshav venous lossis cov ntshav Peripheral. Cov ntshav tag nrho tsis tuaj yeem khaws cia tom qab sau tau. Kuv yuav tsum siv sai sai tom qab sau tau.

2. Cov qauv serum raug sau ua aseptic raws li cov txheej txheem txheem. Cov serum uas tsis ua haujlwm thaum kub tsis tuaj yeem siv. Nws tsis pom zoo kom siv cov serum uas muaj lipemic, turbid lossis muaj kuab paug. Cov khoom me me hauv serum. Thiab cov nag yuav cuam tshuam rau cov txiaj ntsig ntawm kev xeem, cov qauv no yuav tsum tau centrifuged lossis lim ua ntej siv.

3. Cov qauv kuaj tuaj yeem yog heparin, Sodium citrate lossis EDTA anticoagulant plasma.

4. Raws li cov txheej txheem txheem, sau cov qauv. Cov qauv ntshav lossis cov ntshav tuaj yeem khaws cia rau hauv lub tub yees ntawm 2-8 ℃ rau 3 hnub thiab cryopreservation hauv qab -15 ° C rau 3 lub hlis.

5. Tag nrho cov qauv tsis txhob khov thiab yaj.