TSAB NTAWV CAI

Lub membrane ntawm lub cuab yeej kuaj yog coated nrog Rotavirus Group A antigen rau ntawm thaj chaw kuaj thiab tshis tiv thaiv luav IgG antibody rau ntawm thaj chaw tswj. Lub hau npog yog coated los ntawm fluorescence labeled anti Rotavirus Group A thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, RV hauv cov qauv sib xyaw nrog fluorescence labeled anti Rotavirus Group A, thiab tsim cov tshuaj tiv thaiv kab mob. Nyob rau hauv qhov kev ua ntawm immunochromatography, cov dej ntws hauv qhov kev taw qhia ntawm daim ntawv nqus dej. Thaum complex dhau thaj chaw kuaj, nws ua ke nrog antibody tiv thaiv Rotavirus Group A txheej, tsim cov complex tshiab. Yog tias nws tsis zoo, tsis muaj Rotavirus Group A antigen hauv cov qauv, yog li ntawd cov tshuaj tiv thaiv kab mob tsis tuaj yeem tsim, yuav tsis muaj kab liab hauv thaj chaw kuaj pom (T). Txawm hais tias pawg A rotavirus puas muaj nyob hauv cov qauv, tus nas IgG uas muaj latex yog chromatographed rau thaj chaw tswj xyuas zoo (C) thiab ntes los ntawm tshis tiv thaiv nas IgG antibody. Ib kab liab yuav tshwm sim hauv thaj chaw tswj xyuas zoo (C). Kab liab yog tus qauv tshwm sim hauv thaj chaw tswj xyuas zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas yog ib txwm muaj. Nws kuj tseem siv ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov Txheej Txheem Xeem:

1. Yuav tsum tau sau cov neeg mob uas muaj tsos mob. Raws li cov ntawv tshaj tawm, cov kab mob rotavirus uas tawm hauv cov quav ntawm cov neeg mob uas muaj mob plab hnyuv feem ntau tshwm sim 3-5 hnub tom qab pib muaj tus kab mob thiab 3-13 hnub tom qab pib muaj cov tsos mob. Yog tias cov qauv raug sau ntev tom qab raws plab, cov antigens yuav tsis txaus kom tshwm sim qhov tshuaj tiv thaiv zoo.

2. Cov qauv yuav tsum tau sau rau hauv lub thawv huv, qhuav, thiab tsis muaj dej uas tsis muaj cov tshuaj ntxuav thiab cov tshuaj tiv thaiv.

3. Rau cov neeg mob uas tsis raws plab, cov qauv quav uas tau sau yuav tsum tsis pub tsawg dua 1-2 grams. Rau cov neeg mob uas raws plab, yog tias cov quav yog kua, thov sau tsawg kawg 1-2 ml ntawm cov kua quav. Yog tias cov quav muaj ntshav thiab hnoos qeev ntau, thov sau cov qauv dua.

4. Nws raug pom zoo kom kuaj cov qauv tam sim ntawd tom qab sau, txwv tsis pub lawv yuav tsum xa mus rau chav kuaj mob hauv 6 teev thiab khaws cia ntawm 2-8 ° C. Yog tias cov qauv tsis tau kuaj hauv 72 teev, lawv yuav tsum khaws cia ntawm qhov kub hauv qab -15 ° C.

5. Siv cov quav tshiab los kuaj, thiab cov qauv quav sib xyaw nrog cov dej diluent lossis dej distilled.