PRINCIPLE

Lub daim nyias nyias ntawm cov cuab yeej kuaj yog coated nrog FOB tshuaj tiv thaiv nyob rau hauv cheeb tsam xeem thiab tshis anti luav IgG tshuaj tiv thaiv ntawm cheeb tsam tswj. Lable ncoo yog coated los ntawm fluorescence sau los tiv thaiv FOB antibody thiab luav IgG ua ntej. Thaum kuaj cov qauv zoo, FOB antigen hauv cov qauv ua ke nrog cov tshuaj fluorescence sau los tiv thaiv FOB antibody, thiab tsim cov tshuaj tiv thaiv kab mob sib xyaw. Nyob rau hauv qhov kev txiav txim ntawm immunochromatography, complex ntws nyob rau hauv cov kev taw qhia ntawm absorbent daim ntawv. Thaum complex dhau qhov kev xeem cheeb tsam, nws ua ke nrog anti FOB txheej antibody, tsim tshiab complex. Yog tias nws tsis zoo, cov qauv tsis muaj tib neeg hemoglobin lossis cov ntsiab lus tsawg, cov kab mob tsis txaus tiv thaiv tsis tuaj yeem tsim. Yuav tsis muaj kab liab nyob rau hauv qhov chaw kuaj pom (T). Cov kab liab yog tus qauv tshwm nyob rau hauv cheeb tsam tswj qhov zoo (C) rau kev txiav txim seb puas muaj cov qauv txaus thiab seb cov txheej txheem chromatography puas zoo li qub. Nws kuj tseem siv los ua tus qauv tswj sab hauv rau cov tshuaj reagents.

Cov txheej txheem xeem:

Thov nyeem cov pob ntxig ua ntej kuaj.

1. Tshem tawm daim npav xeem los ntawm lub hnab ntawv ci, muab tso rau ntawm lub rooj theem thiab kos nws.

2. Tshem lub hau los ntawm cov qauv raj thiab muab pov tseg thawj ob tee diluted qauv, ntxiv 3 tee (li 100uL) tsis muaj npuas diluted qauv vertically thiab maj mam mus rau hauv cov qauv zoo ntawm daim npav nrog muab dispette. Ces pib lub timer.

3.Qhov tshwm sim yuav tsum tau nyeem nyob rau hauv 10-15 feeb, thiab nws yog invalid tom qab 15 feeb.