Kev kuaj mob cov khoom siv rau progesterone(fluorescence imtimochromatogromographic ensay)
Rau hauv vitro kuaj xyuas siv nkaus xwb
Thov nyeem cov pob ntawv no kom zoo ua ntej siv thiab nruj me ntsis ua raws cov lus qhia. Kev ntseeg tau ntawm Assay cov txiaj ntsig tsis tuaj yeem lav yog tias muaj kev sib txawv los ntawm cov lus qhia hauv cov pob ntawv no.

Siv
Kev kuaj mob cov khoom siv rau progesterone (fluorescence imtochromatographic assay) yog qhov qauv zoo uas yuav tsum tau lees paub los ntawm lwm cov txheej txheem Cov. Qhov kev ntsuas no yog npaj rau kev siv kev noj qab haus huv siv xwb.

Lub ntsiab
Progesterone yog ib qho tseem ceeb uas ua lub luag haujlwm tseem ceeb uas ua lub luag haujlwm tseem ceeb hauv kev tswj hwm ntawm kev coj khaub ncaws thiab yog qhov tseem ceeb hauv kev tswj kom muaj kev xeeb tub. Cov progesterone confentration hauv cov ntshav dej nce sai sai tom qab ovulation. Nws yog qhov qhia tau zoo ntawm lub ntuj ovulation los yog induction ntawm ovulation.

Lub hauv paus ntsiab lus ntawm cov txheej txheem
Daim nyias nyias ntawm cov cuab yeej ntsuas yog coated nrog conjugate nrog bsa thiab prog ntawm thaj chaw xeem thiab tshis los tiv thaiv thaj tsam ntawm cheeb tsam tswj. Cim ntawv ncoo yog coated los ntawm fluorescence Cim los tiv thaiv Prog antibody thiab luav IGG ua ntej. Thaum kuaj cov qauv, prog hauv cov qauv ua ke nrog fluorescence anti prog antibody, thiab kev sib xyaw ua paug. Nyob rau hauv qhov kev txiav txim ntawm immunochnumaphyography, cov complex ntws ntawm cov ntawv xeem, thaum muaj kev nkag siab fluor ntawm lub prog yog qhov tsis zoo rau fluorescence teeb liab, thiab cov concentration ntawm prog nyob rau hauv cov qauv tuaj yeem pom tau los ntawm fluorescence imbunoassay assay.

Reagents thiab khoom siv
25T Pob Cheebtsam:
Kev Xeem Daim Npav Mus Rau Qhov Nyiaj Folded nrog Desiccant 25T
Qauv Diluents 25T
Pob ntxig 1

Cov ntaub ntawv yuav tsum tau tab sis tsis muab
Qauv sau ntim rau, timer

Qauv Sau thiab Cia
1.Qhov kev kuaj mob tuaj yeem yog ntshav, heparin anticoagulant ntshav lossis edta anticoagulant ntshav.
2.Txoj kev sib txuam cov qauv txheej txheem sau cov qauv. Ntshav dej lossis cov qauv ntshav lossis cov qauv ntshav lossis cov qauv ua tub rog tuaj yeem khaws cia hauv tub yees ntawm 2-8 ℃ rau 7days thiab cryoprimerve hauv qab -15 ° C rau 6 lub hlis.
3.Txhua kev coj tsis txhob ua kom tsis txhob khov-thaw cycles.

Txheej txheem assay
Thov nyeem cov lus qhia ua haujlwm thiab cov ntim ntim ua ntej xeem.
1.Txhua txhua yam tshuaj tiv thaiv thiab cov hnoos qeev mus rau hnub sov.
2Qhia lub portable portable portable artalyzer (Wiz-A101), nkag mus rau tus lej nyiaj nkag mus raws qhov ntsuas, thiab nkag mus nrhiav qhov kev tshawb nrhiav interface.
3.Can Cov kws tu hniav rau cov kws kho hniav kom paub meej tias cov khoom xeem.
4.Txhua daim npav xeem los ntawm lub hnab nyiaj.
5.insert daim npav xeem rau hauv daim npav qhov, scan tus QR code, thiab txiav txim siab cov khoom xeem.
6.add 20μl Ntshav lossis Plasma piv txwv rau cov qauv rau DILUENT, thiab sib tov zoo ..
7.Add 80μK Qauv daws teeb meem rau cov qauv zoo ntawm daim npav.
8.Clish "tus qauv xeem", tomqab 10 feeb, qhov ntsuas yuav pom tau cov txiaj ntsig los ntawm cov khoom siv tshuaj ntsuam, thiab sau cov ntawv siv / luam tawm cov ntawv xeem.
9.Refer rau kev qhia ntawm lub portable ageler (wiz-a101).

Kuaj cov qhabnias thiab kev txhais lus

.Qhov cov ntaub ntawv saum toj no yog qhov tshwm sim ntawm kev sim siab, thiab nws tau qhia tias txhua lub chaw kuaj xyuas yuav tsim nyog rau cov pejxeem hauv cheeb tsam no. Cov txiaj ntsig saum toj no yog rau siv xwb.
.Qhov cov txiaj ntsig ntawm cov qauv no tsuas yog siv rau cov kev siv tau tsim nyob rau hauv hom no, thiab tsis muaj qhov sib piv ncaj qha nrog lwm txoj hauv kev.
Cov. Ua rau cov khoom siv tsis pom kev ua kom pom cov txiaj ntsig tau pom, suav nrog cov laj thawj, kev ua haujlwm thiab lwm yam qauv.

Cia thiab Ruaj Khwv
1.Qhov khoom siv yog 18 lub hlis txee-lub neej txij li hnub tsim khoom. Khaws cov khoom siv tsis tau ntawm 2-30 ° C. Tsis khov. Tsis txhob siv dhau hnub tas sij hawm.
2.Tsis txhob qhib lub hnab ntim khoom mus txog thaum koj tau npaj los ua ib qho kev kuaj mob (kub 2-35 ℃, av noo 40-90%) tsis pub dhau 60 mins sai li sai tau li sai tau.
3Sample diluent yog siv tam sim ntawd tom qab qhib.

Ceeb Toom thiab Ceev Faj
Cov .Qhov khoom siv yuav tsum tau muab khi thiab tiv thaiv tiv thaiv noo noo.
.Txhua cov khoom zoo yuav tsum raug muab los ntawm lwm cov txheej txheem.
.All cov txheej txheem yuav raug kho kom zoo li muaj kuab paug.
.Do tsis siv reagent reagent.
.Do tsis tau sib pauv reagents ntawm cov khoom siv nrog sib txawv ntau tsis tau ..
.Do tsis siv daim npav xeem thiab cov khoom siv pov tseg.
.Misoperation, ntau dhau los yog me me qauv tuaj yeem ua rau muaj kev cuam tshuam.

LKev qog
.As nrog ib qho zoo li cov tshuaj tua kab mob nas, qhov ua tau tshwm sim los ntawm tib neeg kev tawm tsam nas tua kab mob (HAMA) hauv qhov quav. Cov quav dev los ntawm cov neeg mob uas tau txais kev npaj ntawm monoclonhal tshuaj tiv thaiv rau kev kuaj mob lossis kev kho mob tuaj yeem muaj Hama. Cov hnoos qeev no yuav ua rau cov txiaj ntsig zoo lossis tsis tseeb.
.Qhov kev xeem ua tsuas yog rau kev kuaj mob, yuav tsum tsis txhob txiav txim siab ua ke nrog nws cov tsos mob hauv kev kho mob, kev kuaj mob, Epidemiology thiab lwm yam ntaub ntawv Cov.
.This reagent tsuas yog siv rau ntshav thiab ntshav cov kev ntsuam xyuas. Tej zaum nws yuav tsis tau txais qhov tshwm sim raug thaum siv rau lwm qhov qauv xws li cov qaub ncaug thiab zis thiab lwm yam.

Cov yam ntxwv ua tau zoo

REFLERENY
1.Txhua kev cuam tshuam nrog Al.Hama cuam tshuam nrog Murine Monoclonal Antibodys [J].
2.Levinson s.Tus ntawm heterophilic antibodies thiab lub luag haujlwm hauv kev cuam tshuam ntawm immunoassay, 1992,15: 108-114.

Qhov tseem ceeb rau cov cim siv:

	Hauv cov vitro kuaj kho mob kho mob
	Tus tsim
	Khw ntawm 2-30 ℃
	Hnub tag sijhawm
	Tsis txhob rov siv dua
	Ceev faj
	Tham cov lus qhia kev siv

Xiamen Wiz Biotech CO., Ltd
Chaw Nyob: 3-4 pem teb, no.16 lub tsev, Rio-Medhoo Rhiav, 2030 Wengjiao West Txoj Kev, Lub Tsev Kawm Ntawv Hengeviao, 361026, Xiamen, Suav
Tel: + 86-592-6808278
Fax: + 86-592-6808279